HPLC Determination of Enantiomeric Purity of Protected Amino Acid Derivatives Used in Peptide Synthesis

Use of Marfey's reagent and analogs for chiral amino acid analysis: Assessment and applications to natural products and biological systems

“…Variants of MR as well as other CDRs were applied to the determination of the degree of epimerization (commonly referred to as 'racemization') of a large number of synthetic peptides containing coded and non-coded AAs [100,101]. Pre-column derivatization with MR was used for monitoring racemization in biologically active peptides, AAs, their N-and C-protected derivatives, branched polypeptides based on polylysine, and endothiopeptides, and to the detection of stereochemical consequences of side reactions and hydrolysis.…”