HPLC determination of digital fingerprint and reliability of Zhusha Anshen Pills

Abstract

In this paper, we aimed to establish Zhusha Anshen Pills chromatogram fingerprint and evaluate it comprehensively with the of traditional Chinese medicine fingerprint evaluation system. The total information index TQ of chromatographic fingerprint was used to optimize the high‐performance liquid chromatography test conditions, and berberine was used as the reference peak to establish the high‐performance liquid chromatography digital fingerprint of Zhusha Anshen Pills. The latent information characteristics of Zhusha Anshen Pills high‐performance liquid chromatography fingerprint were mined by software, and the quality of samples was controlled within the limit of ±15% of the technical parameters of reference fingerprint. The systematic quantified fingerprint method, the normalized chromatographic fingerprint and the reliability evaluation were also used as multiple controls for Zhusha Anshen Pills. Whether the control of latent information characteristic parameters, systematic quantified fingerprint method or reliability control, they all showed that S1 was significantly different from other batches on the whole, but different from others on the details. Compared with other methods, systematic quantified fingerprint method classification is more clearly, accurate, simple to operate and reliability. It is a worthy quality evaluation method for traditional Chinese medicine.

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“…The TQ value is shown in eqn (9), where A i , t R i and N i represent the area, retention time and theoretical plates in each peak, respectively, 27 R i was the separation between each peak and the first peak, and N r was the theoretical tower plate number of the reference fingerprint peak. 28 The TQ value represented the signal size, signal homogenization and information content, and in general, the larger the TQ value, the better the optimization effect.…”

Evaluating the quality consistency of antiviral oral liquid by high-performance liquid chromatography five-wavelength matched average fusion fingerprint combined with electrochemical fingerprint and ultraviolet spectral quantum fingerprint

“…(7) ) was the objective optimization function in selecting experimental settings. TQ was a measure of the magnitude of the fingerprint signal, the degree of signal homogeneity, and the total information content [32] . A higher TQ value often denoted better chromatographic conditions.…”