HPLC Determination of Aloenin in Leaves and Preparations of Aloe arborescens Mill.

Куркин, В. А.; Ryazanova, T. K.; Shmygareva, A.A.; Glushchenko, Svetlana N.

doi:10.1007/s11094-021-02442-9

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…The biological properties of A. vera are contributed by various components. Aloenin A, aloin, and emodin are some of the components responsible for the beneficial effects of A. vera and numerous methods have been developed for their identification 28,29 . In the present study aloin, aloe‐emodin and aloenin A were chosen for inhibitory activity against porcine pancreatic lipase (PPL).…”

Section: Introductionmentioning

confidence: 99%

“…Aloenin A, aloin, and emodin are some of the components responsible for the beneficial effects of A. vera and numerous methods have been developed for their identification. 28,29 In the present study aloin, aloe-emodin and aloenin A were chosen for inhibitory activity against porcine pancreatic lipase (PPL). Surprisingly, aloin and aloe emodin did not inhibit PPL (data not shown), and the inhibitory performance of aloenin A is discussed further.…”

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confidence: 99%

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Lipase activity inhibited by aloenin A: Glycoside from Aloe vera (L.) Burm. f.—In vitro and molecular docking studies

Deora

Venkatraman

2022

J of Molecular Recognition

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Obesity is taking over many parts of the world and has been identified as the second leading cause of preventable death, with a dramatic increase in prevalence over the last two decades. Pancreatic lipase is a lipid‐digesting enzyme that plays an important role in fat metabolism. Inhibiting pancreatic lipase is an attractive target for obesity treatment. Phytochemicals or bioactive compounds/extracts isolated from medicinal plants offer a promising platform for the development of pancreatic lipase inhibitors. This study aims to characterize and investigate the effect of aloenin A, glycoside found in Aloe vera, as a possible inhibitor of pancreatic lipase in vitro and in silico. A. vera extract had an IC50 value of 0.5472 μg/ml, whereas aloenin A had an IC50 value of 14.95 μg/mL and was found to inhibit in a competitive manner. These findings were supported by molecular docking studies, which revealed that aloenin A binds to the substrate binding site with a binding energy of − 7.16 kcal/mol, and this binding site is stabilized by three hydrogen bonds contributed by Phe77 and Asp79. Our findings suggest that the anti‐hyperlipidemic effects of A. vera on pancreatic lipase can be attributed in part to the presence of aloenin A.

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Section: Introductionmentioning

confidence: 99%

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Lipase activity inhibited by aloenin A: Glycoside from Aloe vera (L.) Burm. f.—In vitro and molecular docking studies

Deora

Venkatraman

2022

J of Molecular Recognition

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Enhanced binding interaction and antibacterial inhibition for nanometal oxide particles activated with Aloe Vulgarize through one-pot ultrasonication techniques

Dzulkharnien,

Rohani,

Tan Kofli

et al. 2024

Bioorganic Chemistry

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The Use of Reference Materials In the Analysis of Medicinal Plant Raw Materials and Herbal Medicinal Products

Ryazanova

Куркин

2023

Stand. obraz.

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The need to improve pharmacopoeial approaches to the standardization of medicinal plant raw materials (MPRMs) and herbal medicinal products (HMPs), and the need to ensure the implementation of the principle of «cross-cutting» standardization in the series «medicinal plant raw material – phyto-substance – herbal medicinal product» determine the relevance of developing new, more rational approaches to the analysis of objects of plant origin.The purpose of the research was the theoretical and experimental substantiation of the use of reference materials in the development of assay procedures on the example of certain types of plant raw materials and products based on it from the standpoint of the chemical composition, stability and physicochemical properties of the biologically active compounds contained in them.Assay of active substances was carried out by high performance liquid chromatography (HPLC) using a Milichrome-6 chromatograph with a spectrophotometric detector in the ultraviolet (UV-) region. The UV spectra were recorded using a Specord 40 spectrophotometer. The measurement results were processed using the WinASPECT and Microsoft Excel 2016 programs.As a result of research, procedures for assay of syringin in the bark of Syringa vulgaris, and in the rhizomes and roots of Eleutherococcus senticosus, rosavin and salidroside in the rhizomes and roots of Rhodiola rosea, arbutin in the leaves of Arctostaphylos uva-ursi and Vaccinium vitis-idaea, isosalipurposide in the flowers of Helichrysum arenarium, the amount of anthracene derivatives in fresh leaves of Aloe arborescens were developed and validated. Spectrophotometric methods for determining the amount of biologically active phenylpropanoids in terms of eleutheroside B (syringin) in MPRMs and HMPs of Eleutherococcus senticosus and the amount of aralosides in the roots of Aralia Manchurian were introduced. Based on the data obtained, the scientific rationale for the use of reference materials of syringin (Syringa vulgaris bark, Eleutherococcus senticosus rhizomes and roots), rosavin and salidroside (Rhodiola rosea rhizomes and roots), the sum of ammonium salts of aralosides (Manchurian aralia root), arbutin (Arctostaphylos uva-ursi and Vaccinium vitis-idaea leaves), mixtures of aloins A and B (Aloe arborescens fresh leaves) in the analysis procedures was provided. The concept of a systematic approach to the analysis of medicinal plant raw materials and products based on it was formulated.

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HPLC Determination of Aloenin in Leaves and Preparations of Aloe arborescens Mill.

Cited by 5 publications

References 10 publications

Lipase activity inhibited by aloenin A: Glycoside from Aloe vera (L.) Burm. f.—In vitro and molecular docking studies

Lipase activity inhibited by aloenin A: Glycoside from Aloe vera (L.) Burm. f.—In vitro and molecular docking studies

Enhanced binding interaction and antibacterial inhibition for nanometal oxide particles activated with Aloe Vulgarize through one-pot ultrasonication techniques

The Use of Reference Materials In the Analysis of Medicinal Plant Raw Materials and Herbal Medicinal Products

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