HPLC determination and NMR structural elucidation of sesquiterpene lactones in Inula helenium

Yan, Hao; H, Shi; Li, Weiwei; Wang, Mengyue; Li, Xiaobo

doi:10.1016/j.jpba.2009.09.032

Cited by 42 publications

(22 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is a flower of the Compositae herbaceous family, whose roots have historically been used as a medicine ( 10 ). This plant has been investigated since the 1970s, and certain studies have reported that it contains >40 different compounds, with the terpenoids being the main components ( 11 - 13 ). Alantolactone is a sesquiterpene lactone extracted from Inula Helenium L .…”

Section: Introductionmentioning

confidence: 99%

Alantolactone induces apoptosis and suppresses migration in MCF‑7 human breast cancer cells via the p38�MAPK, NF‑κB and Nrf2 signaling pathways

Liu

Wang

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

Human breast cancer is a malignant type of cancer with high prevalence. In the present study, the anticancer effects of alantolactone, a sesquiterpene lactone, on the human breast cancer cell line MF-7 were investigated in vitro. The MCF-7 cell morphology changed from diamond to round subsequent to treatment with alantolactone, and the cell viability reduced significantly compared with that of the control cells. Alantolactone induced apoptosis of MCF-7 cells by regulating the protein expression levels of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein, p53, caspase-3 and caspase-12, which are associated with the apoptotic pathway, and suppressed colony formation and migration by regulating the protein expression of matrix metalloproteinase (MMP)-2, MMP-7 and MMP-9. Cell signaling pathway analysis confirmed that alantolactone increased the phosphorylation of p38, and decreased the nuclear expression levels of p65 and nuclear factor erythroid 2-related factor 2 (Nrf2), suggesting that the apoptosis-promoting and migration-suppressing effect of alantolactone may partially depend on regulating the p38 MAPK, NF-κB and Nrf2 pathways. These results also suggested that alantolactone may become a potential therapeutic strategy for treating breast cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Alantolactone induces apoptosis and suppresses migration in MCF‑7 human breast cancer cells via the p38�MAPK, NF‑κB and Nrf2 signaling pathways

Liu

Wang

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…The purity of them was determined to be over 98%. Fraction 4 was found to the mixture of dugesialactone and alloantolactone (Huo et al, ). The samples were diluted in dimethyl sulfoxide and added to the plates serially in culturing medium with a final concentration of 0.05%.…”

Section: Methodsmentioning

confidence: 99%

“…I. helenium is a rich source of sesquiterpene lactones, which have attracted attention owing to their wide range of biological activities. Its root contains sesquiterpene lactones including alantolactone, isoalantolactone, igalan, dugesialactone, and alloantolactone (Huo, Shi, Li, Wang, & Li, ). In the previous study, we reported that alantolactone exhibits anticancer activity by suppressing signal transducers and activators of transcription 3 (STAT3) activation (Chun, Li, Cheng, & Kim, ).…”

Section: Introductionmentioning

confidence: 99%

Sesquiterpene lactones‐enriched fraction of Inula helenium L. induces apoptosis through inhibition of signal transducers and activators of transcription 3 signaling pathway in MDA‐MB‐231 breast cancer cells

Chun

Song

Kim

2018

Phytotherapy Research

View full text Add to dashboard Cite

Inula helenium L., commonly known as Elecampane, has been extensively used for many countries in the folk medicine. Its root is a rich source of sesquiterpene lactones, which possess various pharmacological activities. To develop the phytomedicine including sesquiterpene lactones, we prepared hexane fraction from I. helenium (HFIH) and examined the inhibitory effect of HFIH on signal transducers and activators of transcription 3 (STAT3) activation in human breast cancer MDA-MB-231 cells. Additionally, detailed chemical investigation was done to pinpoint the most active sesquiterpene lactones responsible for its anticancer activity.HFIH selectively suppressed STAT3 phosphorylation at tyrosine 705, not affecting its upstream kinases. HFIH downregulated the expression of STAT3 target genes including cyclin D 1 , c-myc, and bcl-2 and induced caspase-mediated apoptosis.Moreover, sesquiterpene lactones of HFIH clearly suppressed STAT3 activation.The in vivo results further supported that HFIH inhibits the growth of human breast xenograft tumors. Our results suggest that HFIH possesses potential anticancer activity, which is mainly mediated through STAT3 signaling pathway. These findings provide the potential of HFIH as a promising phytomedicine for the treatment and prevention of triple-negative breast cancer.

show abstract

“…Additionally, the roots of I. helenium L. are known to improve stomach and liver functions through induction of detoxification enzyme . Previously, it was revealed that the main constituents of its roots are sesquiterpene lactones, including alantolactone and isoalantolactone . These factors have various biological effects such as anti‐inflammatory, antibacterial, and antitumor activities .…”

Section: Introductionmentioning

confidence: 99%

Igalan induces detoxifying enzymes mediated by the Nrf2 pathway in HepG2 cells

Lee

Shin

Chun

et al. 2019

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Igalan is one of the sesquiterpene lactones found in Inula helenium L., which is used as the traditional medicine to treat inflammatory diseases. However, the pharmacological effects of igalan have not been characterized. In this study, we isolated igalan from I. helenium L. and evaluated the effects of igalan on signaling pathways and expression of target genes in HepG2 cells. Igalan activated the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway by increasing the inactive form of GSK3β, the phosphorylated form of AKT, and the nuclear accumulation of Nrf2. Thus, target genes of Nrf2 such as HO-1 and NQO1 increased in HepG2 cells. Moreover, igalan inhibited the tumor necrosis factor-α (TNF-α)-induced nuclear factor-κB activation and suppressed the expression of its target genes, including TNF-α, interleukin (IL)-6, and IL-8 in HepG2 cells. Our results indicate the potential of igalan as an activator of cellular defense mechanisms and a detoxifying agent. K E Y W O R D S detoxification, igalan, liver, nuclear factor-κB, Nrf2 J Biochem Mol Toxicol. 2019;33:e22297.wileyonlinelibrary.com/journal/jbt

show abstract

HPLC determination and NMR structural elucidation of sesquiterpene lactones in Inula helenium

Cited by 42 publications

References 16 publications

Alantolactone induces apoptosis and suppresses migration in MCF‑7 human breast cancer cells via the p38�MAPK, NF‑κB and Nrf2 signaling pathways

Alantolactone induces apoptosis and suppresses migration in MCF‑7 human breast cancer cells via the p38�MAPK, NF‑κB and Nrf2 signaling pathways

Sesquiterpene lactones‐enriched fraction of Inula helenium L. induces apoptosis through inhibition of signal transducers and activators of transcription 3 signaling pathway in MDA‐MB‐231 breast cancer cells

Igalan induces detoxifying enzymes mediated by the Nrf2 pathway in HepG2 cells

Contact Info

Product

Resources

About