HPLC-DAD profiling and inhibitory potentials of ethylacetate and aqueous extracts of <i>Talinum triangulare</i> on key enzymes linked to type-2 diabetes (α-amylase and α-glucosidase) and oxidative stress (monoamine oxidase)

Afolabi, Olakunle Bamikole; Oloyede, Omotade Ibidun; Agunbiade, Oludare S.; Obafemi, Tajudeen Olabisi; Aline, B.; Obajuluwa, Adejoke Olukayode; Jaiyesimi, Kikelomo Folake; Anadozie, Scholastica Onyebuchi

doi:10.1080/2314808x.2019.1670425

Cited by 4 publications

(2 citation statements)

References 35 publications

(36 reference statements)

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“…The plant is commonly utilized in the preparation of soups and other culinary delicacies [24]. Research studies have revealed that the plant is rich in natural compounds like flavonoids and polyphenols [25,26]. The plant has exhibited a wide range of biological and pharmaceutical properties, including anti-inflammatory, anti-fungal, neuroprotective, and anti-bacterial activities [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…The plant has exhibited a wide range of biological and pharmaceutical properties, including anti-inflammatory, anti-fungal, neuroprotective, and anti-bacterial activities [27][28][29]. Additionally, in our previous studies, we reported the antioxidant properties [30], and presence of a number of bioactive compounds identified from the aqueous extract of Tt [25]. However, there is a paucity of information on computational interactions of these compounds with AChE and BChE activities in the prevention/management of neurodegeneration diseases such as AD.…”

Section: Introductionmentioning

confidence: 99%

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Insight into antioxidant-like activity and computational exploration of identified bioactive compounds in Talinum triangulare (Jacq.) aqueous extract as potential cholinesterase inhibitors

Afolabi,

Olasehinde,

Owolabi

et al. 2024

BMC Complement Med Ther

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Background Recent reports have highlighted the significance of plant bioactive components in drug development targeting neurodegenerative disorders such as Alzheimer’s disease (AD). Thus, the current study assessed antioxidant activity and enzyme inhibitory activity of the aqueous extract of Talinum triangulare leave (AETt) as well as molecular docking/simulation of the identified phytonutrients against human cholinesterase activities. Methods In vitro assays were carried out to assess the 2,2- azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) cation radicals and cholinesterase inhibitory activities of AETt using standard protocols. High performance liquid chromatography coupled with diode-array detection (HPLC–DAD) was employed to identify compounds in AETt. Also, for computational analysis, identified bioactive compounds from AETt were docked using Schrodinger's GLIDE against human cholinesterase obtained from the protein data bank (https://www.rcsb.org/). Results The results revealed that AETt exhibited a significant concentration-dependent inhibition against ABTS cation radicals (IC50 = 308.26 ± 4.36 µg/ml) with butylated hydroxytoluene (BHT) as the reference. Similarly, AETt demonstrated a significant inhibition against acetylcholinesterase (AChE, IC50 = 326.49 ± 2.01 µg/ml) and butyrylcholinesterase (BChE, IC50 = 219.86 ± 4.13 µg/ml) activities with galanthamine as the control. Molecular docking and simulation analyses revealed rutin and quercetin as potential hits from AETt, having showed strong binding energies for both the AChE and BChE. In addition, these findings were substantiated by analyses, including radius of gyration, root mean square fluctuation, root mean square deviation, as well as mode similarity and principal component analyses. Conclusion Overall, this study offers valuable insights into the interactions and dynamics of protein–ligand complexes, offering a basis for further drug development targeting these proteins in AD.

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