HPLC-DAD phenolics screening and in vitro investigation of haemostatic, antidiabetic, antioxidant and photoprotective properties of Centaurea tougourensis Boiss. & Reut.

Abstract

Summary Introduction Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine. Objective To investigate the phytochemical and pharmacological properties of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis. Methods The phytochemical evaluation was done based on HPLC-DAD approach. The antioxidant activity was determined by DPPH and cupric ion reducing antioxidant capacity (CUPRAC), while the hemostatic effect was performed using plasma recalcification time (PRT) method. The antidiabetic capacity was investigated by alpha-amylase inhibition assay and the photoprotective test was evaluated by the measurement of sun protection factor (SPF). Results 13 phenolic compounds were identified in both extracts of C. tougourensis. These extracts showed antioxidant, haemostatic, antidiabetic and photoprotective properties with a dose-dependent manner. Amounts of n-BuOH activities were found higher, with a respective IC50 value of 0.72±0.07 μg/ml in DPPH assay, an A0.50 value lower than 3.125 μg/ml in CUPRAC assay besides a shortening rate percentage of coagulation (86.71%) in haemostatic assay, a moderate inhibition effect on alpha amylase activity with an IC50 value of (711.5±0.03 μg/ml) and a maximum sun protection factor of (56.035). These results were mostly found highly significant (p<0.001) when compared to respective standards. Conclusion This study demonstrated some pharmacological effects of C. tougourensis which suggests that our plant could be a good candidate to treat some illnesses related to oxidative stress, bleeding or skin cancer.

Phytochemical Analysis Using cLC-DAD, Nutritional Importance and Assessment of Antioxidant, Antidiabetic and Anticholinesterase Activities of Ruta tuberculata Forssk Organic Extracts

“…Among the diver antioxidant mechanisms, the hydrogen or hydroxyl groups contained in the phenolic structures succeed easily in the transfer of the acidic protons to the free radicals, which leads to stabilizing them effectively. Corollary, the formation of a stable phenoxyl radical resulting from the delocalization of the unpaired electron, as documented par several data [2,30,45]. Furthermore, the significant antioxidant effect of the studied extracts against the free radical may be correlated to the flavonoid compounds previously detected in them.…”

“…Furthermore, these bioflavanones could enhance GLUT-2 and 4 transporters expression and attenuate the 6-P dehydrogenase, 6-PG dehydrogenase, hexokinase, and glucokinase function in pancreatic tissue. In addition, they may down regulate the insulin receptor (IR), PI3kinase, AMPKinase and IL-1β expression levels in diabetic rats which alters the IP/PI3K and AMPK signaling pathways and remedies insulin resistance [2,3,58].…”

“…In this metabolic disorder, the blood glucose level increases markedly as a result of insufficient insulin synthesis or secretion or the inefficiency in binding to its receptor (IR). Indeed, children, adults, and pregnant women were the frequent targets of types (1,2), and gestational diabetes [2,3]. Indeed, it has been documented that hyperglycemia but also fasting hypoglycemia may enhance the Alzheimer's incidence in T2DM cases [4].…”

In Silico Investigation of Some Compounds from the N-Butanol Extract of Centaurea tougourensis Boiss. & Reut.

“…These details are crucial because melanoma is the most dangerous type of skin cancer [62], and the ozone layer's depletion makes people more vulnerable to harmful UVB sun rays [63]. Note that the actual data reported in this study could partially explain the remarkable photoprotective effect of the n-BuOH extract of C. tougourensis reported in the previous study [64]. Compound 1 exhibited a modest cytotoxic effect against acute leukemic T-cells (Jurkat) cell line (Pa = 0.456), which may show promise in the treatment of diseases related to hematopoiesis since, in a leukemia situation, bone marrow is replaced with tumors, which prevents the regular producing of B or T lymphocytes and may cause long-term anemia [65,66].…”

HPLC-DAD phenolic screening and in vitro assessment of antimicrobial, antioxidant and anti-inflammatory activities of Tanteboucht dates

“…Detection was performed at three different wavelengths, 250 nm, 280 nm and 340 nm. 10 2.5. Tests of the biological activities of the extracts 2.5.1.…”