HPLC-DAD-ESI-MS/MS characterization of bioactive secondary metabolites from Strelitzia nicolai leaf extracts and their antioxidant and anticancer activities In vitro

Ghareeb, Mosad A.; Saad, Amal M.; Ahmed, Wafaa Sabry; Refahy, Laila A.; Nasr, Sami Mohamed

doi:10.4103/pr.pr_89_18

Cited by 22 publications

(10 citation statements)

References 2 publications

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“…Over-production and the accumulation of free radicals inside the body leads to a phenomenon known as oxidative stress, which causes many serious diseases such as cancer, cardiovascular, and inflammation. Moreover, the harmful effects of this phenomenon can be reduced via using antioxidant compounds as free radical scavengers [ 36 , 37 , 38 , 39 ]. Therefore, free radical scavenging capabilities were determined for all compounds by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay using ascorbic acid (AA) as standard for comparison.…”

Section: Resultsmentioning

confidence: 99%

In Vitro and In Vivo Antitumor Activity of Indolo[2,3-b] Quinolines, Natural Product Analogs from Neocryptolepine Alkaloid

et al. 2021

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Neocryptolepine (5-methyl-5H-indolo[2,3-b] quinoline) analogs were synthesized and evaluated in vitro and in vivo for their effect versus Ehrlich ascites carcinoma (EAC). The analogs showed stronger cytotoxic activity against EAC cells than the reference drug. The in vivo evaluation of the target compounds against EAC-induced solid tumor in the female albino Swiss mice revealed a remarkable decrease in the tumor volume (TV) and hepatic lipid peroxidation. A noticeable increase of both superoxide dismutase (SOD) and catalase (CAT) levels was reported (p < 0.001), which set-forth proof of their antioxidant effect. In addition, the in vitro antioxidant activity of the neocryptolepine analogs was screened out using the DPPH method and showed promising activities activity. The histopathological investigations affirmed that the tested analogs have a remarkable curative effect on solid tumors with minimal side-effect on the liver. The study also includes illustrated mechanism of the antitumor activity at the cell level by flow cytometry. The cell cycle analysis showed that the neocryptolepine analogs extensively increase the aggregation of tumor cells in three phases of the cell cycle (G0/G1, S and G2/M) with the emergence of a hypo-diploid DNA content peak (sub-G1) in the cell cycle experiments, which is a clear-cut for the apoptotic cell population. Furthermore, the immunological study manifested a significant elevation in splenic lymphocyte count (p < 0.001) with the elevation of the responsiveness of lymphocytes to phytohemagglutinin (PHA). These results indicate that these naturally-based neocryptolepine alkaloids exhibit marked antitumor activity in vivo and represent an important lead in the development of natural-based anticancer drugs.

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Section: Resultsmentioning

confidence: 99%

In Vitro and In Vivo Antitumor Activity of Indolo[2,3-b] Quinolines, Natural Product Analogs from Neocryptolepine Alkaloid

et al. 2021

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“…* HPLC-DAD-ESI-MS-MS annotation of the polyphenolic compounds based on their retention time, fragmentation pattern, and via comparison of MS spectra with the reported data [34,35,36,37,38,39,40,41,42,43,44]. a Compounds isolated and identified via HPLC-DAD-ESI-MS/MS during this study.…”

Section: Figurementioning

confidence: 99%

Chemical Profiling of Polyphenolics in Eucalyptus globulus and Evaluation of Its Hepato–Renal Protective Potential Against Cyclophosphamide Induced Toxicity in Mice

et al. 2019

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Cyclophosphamide (CP) is a potent anti-neoplastic and immunosuppressive agent; however, it causes multi-organ toxicity. We elucidated the protective activities of Eucalyptus globulus (EG) leaf extract against CP-induced hepato–renal toxicity. Mice were treated with EG for 15 days plus CP on day 12 and 13 of the experiment. Using HPLC-DAD-ESI-MS/MS, 26 secondary metabolites were identified in EG leaf extract. Out of them, 4 polyphenolic compounds were isolated: (1) 4-(O-β-d-xylopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (2) 4-(O-α-l-rhamnopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (3) gallic acid, and (4) methyl gallate. Effects of EG extract on biochemical parameters, gene expression, and immune-histopathological changes were assessed in comparison to mesna positive control. Results showed that EG improved CP-increased serum ALT, AST, creatinine, and blood urea nitrogen levels. The hepatic and renal tissue levels of MDA, nitric oxide, protein carbonyl, TNF-α, IL-6, and immunohistochemical expression of nuclear factor kappa-B (NF-kB) and caspase-3 were reduced. Also, hepatic and renal GSH contents, and nuclear factor E2-related factor 2 (NRf2)/ hemoxygenase-1 (HO-1) signaling levels were increased. Histopathological findings supported our findings where hepatic and renal architecture were almost restored. Results revealed the protective effects of EG against CP-induced hepato–renal toxicity. These effects may be related to EG antioxidant, anti-inflammatory, and anti-apoptotic properties coupled with activation of Nrf2/HO-1 signaling.

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“…Numerous previous reports revealed the antioxidant activity of medicinal plants based on the presence of certain polyphenolic compounds including phenolic acids, flavonoids, tannins, and their derivatives. [28][29][30]…”

Section: Antioxidant Activity and Total Phenolic Contentmentioning

confidence: 99%

Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of <i>Artemisia campestris</i> from Algeria

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Gherib

Bronze

et al. 2019

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Amaç: Bu çalışmada, Cezayir'de yetişen Artemisia campestris'in sulu alkollü ekstresinin kimyasal profilinin ve antioksidan etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Cezayir'de yetişen A. campestris'in sulu alkollü ekstresinin fenolik bileşenleri yüksek performanslı sıvı kromatografisi (HPLC) diyot dizinli dedektör-(DAD)-elektrosprey iyonizasyonu (ESI)-mass spektrometresi (MS)/MS kullanılarak incelenmiştir. İn vitro antioksidan aktivite ve toplam fenolik içerik de sırasıyla oksijen radikal absorbans kapasitesi ve Folin-Ciocalteu analizleri ile değerlendirilmiştir. Bulgular: HPLC-DAD-ESI-MS/MS analizi, esas olarak saptanan ana bileşiklerin, kafeoilkuinik asit izomerleri, flavonoitler ve benzoik asit türevleri olduğunu ortaya koymuştur. Bununla birlikte, sulu alkollü ekstre 120.5±10.4 μmol Trolox eşdeğeri antioksidan kapasitesi/g kuru ağırlık (KA) değeri ile önemli derecede antioksidan aktivite göstermiş ve bu aktivite ile 102.09±1.65 mg/g gallik asit eş değeri KA olduğu belirlenen toplam fenolik içerik değeri arasında güçlü bir korelasyon saptanmıştır. Sonuç: Sonuç olarak, A. campestris'in sulu alkollü ekstresinin, doğal antioksidan ajanların üretimi için umut verici bir aday olduğu belirtilmiştir. Anahtar kelimeler: Artemisia campestris, polifenoller, flavonoitler, klorojenik asit, antioksidan Objectives: Our study aimed to investigate the chemical profile of hydroalcoholic extract of Algerian Artemisia campestris and its antioxidant activity. Materials and Methods: The hydroalcoholic extract of Algerian A. campestris was investigated for its phenolic constituents using high performance liquid chromatography (HPLC)-diode array detection (DAD)-electrospray ionization (ESI)-mass spectrometer (MS)/MS. The in vitro antioxidant activity and total phenolic content were also evaluated via oxygen radical absorbance capacity and Folin-Ciocalteu assays, respectively. Results: HPLC-DAD-ESI-MS/MS analysis revealed that the main tentatively identified compounds were caffeoylquinic acid isomers, flavonoids, and benzoic acid derivatives. Additionally, the hydroalcoholic extract exhibited a promising antioxidant activity value of 120.5±10.4 μmol Trolox equivalent antioxidant capacity/g dry weight (DW), and a strong correlation exists between this activity and the total phenolic content value of 102.09±1.65 mg/g gallic acid equivalents DW. Conclusion: The hydroalcoholic extract of A. campestris is a promising candidate for the production of naturally occurring antioxidant agents.

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HPLC-DAD-ESI-MS/MS characterization of bioactive secondary metabolites from Strelitzia nicolai leaf extracts and their antioxidant and anticancer activities In vitro

Cited by 22 publications

References 2 publications

In Vitro and In Vivo Antitumor Activity of Indolo[2,3-b] Quinolines, Natural Product Analogs from Neocryptolepine Alkaloid

In Vitro and In Vivo Antitumor Activity of Indolo[2,3-b] Quinolines, Natural Product Analogs from Neocryptolepine Alkaloid

Chemical Profiling of Polyphenolics in Eucalyptus globulus and Evaluation of Its Hepato–Renal Protective Potential Against Cyclophosphamide Induced Toxicity in Mice

Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of <i>Artemisia campestris</i> from Algeria

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