HPLC-based activity profiling for antiplasmodial compounds in the traditional Indonesian medicinal plant Carica papaya L

Julianti, Tasqiah; Mieri, Maria De; Zimmermann, Stefanie; Ebrahimi, Samad Nejad; Kaiser, Marcel; Neuburger, Markus; Raith, Melanie; Brun, Reto; Hamburger, Matthias

doi:10.1016/j.jep.2014.05.050

Cited by 50 publications

(27 citation statements)

References 33 publications

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“…Carpaine was identified in the active fraction and dichloromethane leaf extract using LC-MS (data not shown). The antimalarial activity of carpaine is consistent with that reported by Julianti et al who tested carpaine against P. falciparum K1 (resistant to chloroquine and pyrimethamine), although the compound did not prolong survival times in P. berghei-infected mice [10]. It should also be noted that there are reports showing differing susceptibilities between P. falciparum and P. berghei to some compounds, such as the natural products cryptolepine and berberine.…”

Section: Antimalarial Activity Of C Papaya Leaf Extracts Fractions supporting

confidence: 88%

In vitro Antimalarial Evaluations and Cytotoxicity Investigations of Carica papaya Leaves and Carpaine

Teng

Chan

Suwanarusk

et al. 2019

Natural Product Communications

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The objective of this study is to evaluate the antimalarial property of Carica papaya L. leaf extracts and the cytotoxicity of active samples. C. papaya leaves were extracted and screened against Plasmodium falciparum 3D7 and Dd2 strains. Bioassay-guided fractionation was carried out. The dichloromethane extract of C. papaya leaves showed significant antiplasmodial activity against P. falciparum 3D7 and Dd2. Successful bioassay-guided fractionation afforded a fraction three to seven times more active than the dichloromethane extract. Carpaine was isolated from the most active alkaloidal extract and identified in the active fraction and dichloromethane leaf extract. The cytotoxicity of active samples was evaluated against NL20 cells. A haemolysis assay was performed on carpaine. Carpaine exhibited good activity against both strains of P. falciparum with IC 50 of 2.01 ± 0.18 μg/mL (4.21 µM) and 2.19 ± 0.60 μg/mL (4.57 µM) against 3D7 and Dd2 strains respectively. It exhibited high selectivity for the parasite and was non-toxic to healthy uninfected human red blood cells. This is the first study investigating the haemolysis potential of carpaine. The results provide a scientific basis for the traditional use of C. papaya leaves for malaria treatment. More work is required to evaluate the efficacy and safety of carpaine for further development into potential new antimalarial drugs.

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Section: Antimalarial Activity Of C Papaya Leaf Extracts Fractions supporting

confidence: 88%

In vitro Antimalarial Evaluations and Cytotoxicity Investigations of Carica papaya Leaves and Carpaine

Teng

Chan

Suwanarusk

et al. 2019

Natural Product Communications

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“…No obvious dependence on geographic origin and leaf maturity was observed. Quantification of the Antiplasmodial Alkaloid Carpaine in Papaya (Carica papaya) Leaves were able to corroborate this finding by in vitro testing of papaya extracts, and with the aid of HPLC-based activity profiling [12], we identified the alkaloid carpaine as the major antiplasmodial compound [13]. Carpaine possessed significant antiplasmodial activity in vitro (IC 50 of 0.2 µM) and high selectivity towards the parasite.…”

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confidence: 63%

“…UPLC/MS grade ACN, MeOH, formic acid, TFA, acetone, and IPA were from BioSolve. Carpaine was previously isolated in our laboratory from papaya leaves [13]. A purity of > 96 % was determined by HPLC-MS and NMR.…”

Section: Chemicals and Reference Compoundsmentioning

confidence: 99%

Quantification of the Antiplasmodial Alkaloid Carpaine in Papaya (Carica papaya) Leaves

2014

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Daily consumption of papaya (Carica papaya) leaves as greens and an herbal infusion is common in some parts of Indonesia as a means for preventing malaria. Antiplasmodial activity of the leaf extracts and of the main alkaloid carpaine were recently confirmed. A quantitative assay for determination of carpaine in papaya leaves was developed and validated. The assay involved pressurized liquid extraction and quantification with the aid of ultrahigh-performance liquid chromatography-tandem mass spectroscopy. Extraction conditions were optimized with respect to solvent, temperature, and number of extraction cycles. The ultrahigh-performance liquid chromatography-tandem mass spectroscopy assay was validated over a range of 20-5000 ng/mL (R(2) of 0.9908). A total of 29 papaya leaf samples were analyzed, and carpaine concentration in dry leaves was found to range from 0.02 to 0.31%. No obvious dependence on geographic origin and leaf maturity was observed.

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“…There are significant differences between both extracts in the concentrations of some particular polyphenols, like rutin and kaempferol, being higher in the HO P , which is the more efficient one. For this reason, we could infer a potential action of these specific polyphenols which have already been described as antimalarial agent in different studies (Julianti et al 2014;Ganesh et al 2012;Silveira et al 2009;Somsak et al 2018).…”

Section: Parasitesmentioning

confidence: 98%

Exploring the powerful phytoarsenal of white grape marc against bacteria and parasites causing significant diseases

Rama

Mallo

Biddau

et al. 2020

Environ Sci Pollut Res

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Natural extracts containing high polyphenolic concentration possess antibacterial, anti-parasitic and fungicidal activities. The present research characterises two extracts based on white grape marc, a winemaking by-product, describing their physicochemical features and antimicrobial capacities. The main components of these extracts are phenolic acids, flavan-3-ols and their gallates and flavonols and their glycosides. As a result of this complex composition, the extracts showed pronounced bioactivities with potential uses in agricultural, pharmaceutical and cosmetic industries. Polyphenol compounds were extracted by using hydro-organic solvent mixtures from the by-product of Albariño white wines (Galicia, NW Spain) production. The in vitro antimicrobial activity of these extracts was evaluated on Grampositive and Gram-negative bacteria and Apicomplexan and Oomycota parasites. Microbial species investigated are causing agents of several human and animal diseases, such as foodborne illnesses (Bacillus cereus, Escherichia coli, Salmonella enterica, and Toxoplasma gondii), skin infections and/or mastitis (Staphylococcus aureus and Streptococcus uberis), malaria (Plasmodium falciparum) and plant infections as "chestnut ink" or "root rot" (Phytophthora cinnamomi). Both extracts showed activity against all the tested species, being nontoxic for the host. So, they could be used for the development of biocides to control a wide range of pathogenic agents and contribute to the enhancement of winemaking industry by-products.

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HPLC-based activity profiling for antiplasmodial compounds in the traditional Indonesian medicinal plant Carica papaya L

Cited by 50 publications

References 33 publications

In vitro Antimalarial Evaluations and Cytotoxicity Investigations of Carica papaya Leaves and Carpaine

In vitro Antimalarial Evaluations and Cytotoxicity Investigations of Carica papaya Leaves and Carpaine

Quantification of the Antiplasmodial Alkaloid Carpaine in Papaya (Carica papaya) Leaves

Exploring the powerful phytoarsenal of white grape marc against bacteria and parasites causing significant diseases

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