HPLC Assay of Tomato Carotenoids: Validation of a Rapid Microextraction Technique

Sérino, Sylvie; Gomez, Laurent; Costagliola, Guy; Gautier, Hélène

doi:10.1021/jf902113n

Cited by 88 publications

(51 citation statements)

References 33 publications

(44 reference statements)

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“…Carotenoid extraction was modified from Sérino et al (2009). Aliquots of 200 mg fresh weight were extracted sequentially with a solution of saturated NaCl, dichloromethane, and hexane:diethyl ether (1:1).…”

Section: Tocopherol and Pigment Quantificationmentioning

confidence: 99%

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

et al. 2017

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Senescence is the process that marks the end of a leaf's lifespan. As it progresses, the massive macromolecular catabolism dismantles the chloroplasts and, consequently, decreases the photosynthetic capacity of these organs. Thus, senescence manipulation is a strategy to improve plant yield by extending the leaf's photosynthetically active window of time. However, it remains to be addressed if this approach can improve fleshy fruit production and nutritional quality. One way to delay senescence initiation is by regulating key transcription factors (TFs) involved in triggering this process, such as the NAC TF ORESARA1 (ORE1). Here, three senescence-related NAC TFs from tomato (Solanum lycopersicum) were identified, namely SlORE1S02, SlORE1S03, and SlORE1S06. All three genes were shown to be responsive to senescence-inducing stimuli and posttranscriptionally regulated by the microRNA miR164. Moreover, the encoded proteins interacted physically with the chloroplast maintenance-related TF SlGLKs. This characterization led to the selection of a putative tomato ORE1 as target gene for RNA interference knockdown. Transgenic lines showed delayed senescence and enhanced carbon assimilation that, ultimately, increased the number of fruits and their total soluble solid content. Additionally, the fruit nutraceutical composition was enhanced. In conclusion, these data provide robust evidence that the manipulation of leaf senescence is an effective strategy for yield improvement in fleshy fruit-bearing species.

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Section: Tocopherol and Pigment Quantificationmentioning

confidence: 99%

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

et al. 2017

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“…Caballo et al (2012) proposed a microextraction of carotenoids using a supramolecular solvent consisting of decanoic acid assemblies to simplify carotenoid determination in farmed salmonids, but this is a specific method for ketocarotenoids. Sérino et al (2009) suggested a microextraction of tomato carotenoids (lutein, lycopene, b-carotene, and phytoene) using sodium chloride, n-hexane, dichloromethane, and ethyl acetate.…”

Section: Introductionmentioning

confidence: 99%

Microscale extraction method for HPLC carotenoid analysis in vegetable matrices

Pacheco

Peixoto

Borguini

et al. 2014

Sci. agric. (Piracicaba, Braz.)

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In order to generate simple, efficient analytical methods that are also fast, clean, and economical, and are capable of producing reliable results for a large number of samples, a micro scale extraction method for analysis of carotenoids in vegetable matrices was developed. The efficiency of this adapted method was checked by comparing the results obtained from vegetable matrices, based on extraction equivalence, time required and reagents. Six matrices were used:

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“…β-carotene content was determined by a spectrophotometric method with samples being subjected to saponification with potassium hydroxide water -alcohol solution, vitamin extraction with diethyl ether, vitamin separation by chromatography on an aluminum oxide column, and quantitative determination of vitamins by photometric method (Sérino et al, 2009).…”

Section: Nutritional Compositionmentioning

confidence: 99%

Technology development of protein rich concentrates for nutrition in extreme conditions using soybean and meat by-products

Каленик

Costa

Motkina

et al. 2017

Acta Sci Pol Technol Aliment

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Background. There is a need to develop new foods for participants of expeditions in extreme conditions, which must be self-sufficient. These foods should be light to carry, with a long shelf life, tasty and with high nutrient density. Currently, protein sources are limited mainly to dried and canned meat. In this work, a protein-rich dried concentrate suitable for extreme expeditions was developed using soya, tomato, milk whey and meat by-products. Materials and methods. Protein concentrates were developed using minced beef liver and heart, dehydrated and mixed with a soya protein-lycopene coagulate (SPLC) obtained from a solution prepared with germinated soybeans and mixed with tomato paste in milk whey, and finally dried. The technological parameters of pressing SPLC and of drying the protein concentrate were optimized using response surface methodology. Results. The optimized technological parameters to prepare the protein concentrates were obtained, with 70:30 being the ideal ratio of minced meat to SPLC. The developed protein concentrates are characterized by a high calorific value of 376 kcal/100 g of dry product, with a water content of 98 g·kg -1 , and 641-644 g·kg

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HPLC Assay of Tomato Carotenoids: Validation of a Rapid Microextraction Technique

Cited by 88 publications

References 33 publications

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

Microscale extraction method for HPLC carotenoid analysis in vegetable matrices

Technology development of protein rich concentrates for nutrition in extreme conditions using soybean and meat by-products

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