HPLC and tandem detection to monitor conformational properties of biopharmaceuticals

Luykx, Dion M.A.M.; Goerdayal, Soenita S.; Dingemanse, Pieter J.; Jiskoot, Wim; Jongen, Peter M.J.M.

doi:10.1016/j.jchromb.2005.04.005

Cited by 26 publications

(11 citation statements)

References 19 publications

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“…However, elution could be affected by the shape of the compound. In the case of IEC, compounds are separated on the basis of charge; low-charged compounds elute faster than highly charged ones (Luykx et al 2005(Luykx et al , 2008. HPLC is a form of liquid chromatography to separate, analyse, and quantify compounds that are dissolved in solution.…”

Section: Chromatographymentioning

confidence: 99%

Nanoemulsions for Food Applications: Development and Characterization

Silva

Cerqueira

2011

Food Bioprocess Technol

534

216

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The application of nanotechnology to food, medical and pharmaceutical industries has received great attention from the scientific community. Driven by the increasing consumers' demand for healthier and safer food products and the need for edible systems able to encapsulate, protect, and release functional compounds, researchers are currently focusing their efforts in nanotechnology to address issues relevant to food and nutrition. Nanoemulsion technology is particularly suited for the fabrication of encapsulating systems for functional compounds as it prevents their degradation and improves their bioavailability. This review focuses on nanoemulsions and provides an overview of the production methods, materials used (solvents, emulsifiers, and functional ingredients) and of the current analytical techniques that can be used for the identification and characterization of nanoemulsions. Finally, nanotechnological applications in foods currently marketed are reported.

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Section: Chromatographymentioning

confidence: 99%

Nanoemulsions for Food Applications: Development and Characterization

Silva

Cerqueira

2011

Food Bioprocess Technol

534

216

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“…It has been reported to provide some stabilization of ␤-sheet structure in polypeptides. Additionally, in the presence of 60% acetonitrile, the protein interferon alpha-2b has been shown to lose its alpha-helical structure while still retaining its anti-parallel beta-sheets [188][189][190].…”

Section: Organic Modifiermentioning

confidence: 99%

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

Fekete

Veuthey

Guillarme

2012

Journal of Pharmaceutical and Biomedical Analysis

128

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“…These conformational changes seemed to be correlated to the appearance of a peak at 1.5 min for urocortin solutions containing at least 40% (v/v) acetonitrile. In addition, there have been reports showing that organic solvents in a polypeptide solution cause the conformational change in polypeptides (Lin and Karger, 1990;Oroszlan et al, 1992;Wicar et al, 1994;Luykx et al, 2005). In particular, Wicar et al (1994) reported that a change in the 1-propanol content in a recombinant human growth hormone solution induced phase transition of the conformation, and that such solvent-induced conformational change is correlated with the retention changes observed in reversed-phase chromatography.…”

Section: Spectral Analysis Of Urocortin Solutionsmentioning

confidence: 97%

Highly sensitive and quantitative analysis of polyeptides using a new gradient system based on an abrupt change in adsorption of polypeptide to the reversed‐phase column packing

Goda

Sudo

2007

Biomedical Chromatography

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During a study of 100 microL aliquots of urocortin containing various acetonitrile contents, we hypothesized that a change in the acetonitrile content in the solution across a specific content of acetonitrile (critical threshold) causes an abrupt change in adsorption capacity to the column packing. Circular dichroism measurements suggest that the conformational change induced by acetonitrile in the solution causes the abrupt change in adsorption capacity, and this solvent-induced conformational change is reversible across the critical threshold. This hypothesis can apply to various polypeptides with molecular weights range from 1007 to 6789 and to other organic solvents. A new gradient system utilizing the instant recovery of the adsorption capacity across the critical threshold was designed, and applied to the analysis of a 100 microL aliquot of various polypeptide solutions. The results suggest that use of a solution containing organic solvents more than the critical threshold allows successful dilution of polypeptides up to picomolar concentration range without any loss due to its adsorption during handling procedures and loading onto the LC system, and that a new gradient system enables quantitative analysis of polypeptides at picomolar concentrations in such solutions.

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HPLC and tandem detection to monitor conformational properties of biopharmaceuticals

Cited by 26 publications

References 19 publications

Nanoemulsions for Food Applications: Development and Characterization

Nanoemulsions for Food Applications: Development and Characterization

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

Highly sensitive and quantitative analysis of polyeptides using a new gradient system based on an abrupt change in adsorption of polypeptide to the reversed‐phase column packing

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