HPLC and in vivo Spectrophotometric Detection of Porphyrins in Plant Tissues Treated with Porphyrinogenic Herbicides

Abstract

yellow cucum ber cotyledon discs approxim ated the ab so rp tio n spectra o f p ro to p o rp h y rin IX (P ro to IX). T here was also an enhanced peak n ear 503 nm . T reatm ent o f cotyledon discs w ith A L A alone generated a differ ence spectrum o f protochlorophyllide (PChlide) in co m b in atio n with M g -P roto IX or M g-P roto IX m onom ethyl ester (M g-P roto IX M E). W ith A L A a n d A F in co m b in atio n , the PC hlide and M g-P roto IX portions o f the difference spectrum were reduced an d the P roto IX peak an d peak n ear 503 nm were increased. D Y treatm en t yielded a difference spectrum w ith peaks approxim ating those o f Proto IX an d M g-Proto IX M E , along w ith a peak n ear 503 nm . T he presence o f all porp h y rin s detected by in vivo sp ectro p h o to m etry except fo r the 503 nm p eak w as confirm ed w ith H PL C . Proto IX m onom ethyl ester was found by H P L C to be espe cially elevated in treatm ents with AF. The in vivo 503 nm peak a n d in vitro studies w ith Protoxco n tain in g barley etioplast p reparations suggest th a t p ro to te tra h y d ro p o rp h y rin IX (an oxid a tion state interm ediate between proto p o rp h y rin o g en IX an d P roto IX ) m ay accum ulate un d er som e conditions. These d ata dem onstrate th at rapid in vivo spectro p h o to m etric studies can provide m uch o f the qualitative results o f H P L C studies and can confirm th a t in vitro results co rresp o n d with the in vivo situation.

Effects of Phytotoxic Nonenolides, Stagonolide A and Herbarumin I, on Physiological and Biochemical Processes in Leaves and Roots of Sensitive Plants

“…These data suggest that possible differences between MOA of STA and of HBI are influenced by both plant species and toxin structure. Further quantification of isoprenoids and porphyrins in Arabidopsis leaves will allow to conclude whether the effects of these nonenolides are related with pigment biosynthesis [51,52].…”

Chlorophyll Analysis by New High Performance Liquid Chromatography Methods

“…By comparison, most of the methods achieving the separation of free and esterified pigments occurring in algae or etiolated tissues from higher plants show several multicomponent peaks corresponding to the coelution of the [MV]-and [DV]-analogues of a particular Chl derivative (Barry et al, 1991;Wright et al, 1991;Van Heukelem et al, 1992;Duke et al, 1993;Schoefs et al, 1995). Silica-based polymeric stationary phases exhibit very high selectivity towards very similar, even isomeric, compounds with rigid molecular structures, the so-called ' shape selectivity' (Sander et al, 1999).…”