HPLC-Analytik von ethanolischen Eichenholzextrakten mit UV-, Fluorescenz- und amperometrischer Detektion

Liquid Chromatography with On-Line Electrochemical Derivatization and Fluorescence Detection for the Determination of Phenols

“…Phenolic compounds are important analytes in environmental and food chemistry. Several publications deal with the determination of phenols in highly diverse matrixes including water, [1][2][3][4][5][6][7][8] biological fluids, [9][10][11] beverages, [12][13][14] wood extracts, 15 and aviation jet fuel. 16 Most authors rely on high-performance liquid chromatography (HPLC) with UV/visible, fluorescence, and electrochemical detection or combinations thereof.…”

“…16 Most authors rely on high-performance liquid chromatography (HPLC) with UV/visible, fluorescence, and electrochemical detection or combinations thereof. 1,3,4,[9][10][11][14][15][16] More recently, high-performance liquid chromatography with mass spectrometric detection (HPLC/MS) has also been used for phenol determination. 8 Capillary electrophoresis (CE) with fluorescence, 2 amperometric, 5 UV/visible, 13 or MS 6 detection has been described frequently for this purpose as well.…”

“…UV/visible detection even when a diode array detector (DAD) and multiwavelength detection is used can provide neither the selectivity nor the sensitivity required for direct real sample measurement; thus, cumbersome sample preparation has to be carried out. 10,11,13,15 Direct fluorescence detection (FL) is restricted to those phenols that show native fluorescence and the detection limits are dependent on their unfavorable fluorescence properties. 1,10,14,15 Electrochemical detection (ED) is highly sensitive but is adversely influenced by oxidizable matrix components, which are oxidized at the relatively high detection potentials.…”

“…10,11,13,15 Direct fluorescence detection (FL) is restricted to those phenols that show native fluorescence and the detection limits are dependent on their unfavorable fluorescence properties. 1,10,14,15 Electrochemical detection (ED) is highly sensitive but is adversely influenced by oxidizable matrix components, which are oxidized at the relatively high detection potentials. Additionally, when LC separation is desired, gradient elution is not compatible with ED, reducing the separation capacity and lengthening analysis times in LC.…”

“…Several publications deal with the determination of phenols in highly diverse matrixes including water, − biological fluids, − beverages, − wood extracts, and aviation jet fuel . Most authors rely on high-performance liquid chromatography (HPLC) with UV/visible, fluorescence, and electrochemical detection or combinations thereof. ,,,− ,− More recently, high-performance liquid chromatography with mass spectrometric detection (HPLC/MS) has also been used for phenol determination . Capillary electrophoresis (CE) with fluorescence, amperometric, UV/visible, or MS 6 detection has been described frequently for this purpose as well.…”