HPLC Analysis of Rice Bran Oil

Abstract

A simple isocratic reversed phase high‐performance liquid chromatographic method was developed to study lipase‐catalyzed esterification of high free fatty acid (FFA) rice bran oil. The method was used to separate and quantify FFAs, monoacylglycerols, diacylglycerols and triacylglycerols of rice bran oil as different lipid classes. Separation was obtained with a C18 column using acetone/acetonitrile/methanol (50:50:10, v/v/v) as the mobile phase at 30C with refractive index detection.

An indirect analytical approach based on ATR-FTIR spectroscopy for determining the FFA content in vegetable oils

“…[7][8][9][10] Based on chromatographic techniques, some robust methods for resolving FFA in rice bran oil, corn oil, sesame oil and soybean oil were successfully developed. 6,[11][12][13][14] Another high-speed colorimetry method was developed to determine FFA in oils with synthetic polyamidoamine dendrimers and citrate-capped gold nanoparticles. 3 A number of spectroscopic methods, such as UV-vis, H-1-nuclear magnetic resonance, and molecular spectroscopy, are used in the detection of FFA in vegetable oils.…”

A Comprehensive Study on Triacylglycerols in Tobacco Leaves Using Liquid Chromatography and Atmospheric- Pressure Chemical-Ionization Mass Spectrometry

“…The reason for the frequent use of this combination is that non-aqueous reverse-phase chromatography (NARPC), a separation technique for reverse-phase liquid chromatography without water, was expected to efficiently separate low polarity components (11). For this reason, the following detectors have been coupled with the separation system for triacylglycerols: refractive index detector (RID) (12), evaporative light scattering detector (ELSD) (13)(14)(15), atmospheric pressure chemical ionization mass spectrometer (APCI-MS) (10,(16)(17)(18)(19)(20)(21)(22) and atmospheric pressure photoionization mass spectrometer (APPI-MS) (23). Above all, it should be noted that APCI-MS is a highly effective instrument for the identification of triacylglycerols.…”

Conversão enzimática de triacilgliceróis em mono e diacilgliceróis de interesse industrial

“…Componentes separados podem ser recuperados a partir da fase móvel para posterior caracterização por índice de refração (RID), espectrometria de massa, ressonância magnética nuclear e por espectroscopia do infravermelho (IV). Além disso, vários solventes e materiais para empacotamento de coluna proporcionam um elevado grau de seletividade para uma análise específica(KADAM & BHOWMICK, 2006). Como uma especialização de HPLC, a cromatografia de permeação em gel, também chamada cromatografia de exclusão de tamanho de alto desempenho (HPSEC), é uma técnica apropriada aos estudos de grupos lipídicos, considerando sua simplicidade e sua alta confiabilidade.…”