HPLC Analysis of Plasma Glipizide and its Application to Pharmacokinetic Study

Bae, Jung‐Woo; Kim, Nam-Tae; Choi, Chang‐Ik; Kim, Mi-Jeong; Jang, Choon‐Gon; Lee, Seok‐Yong

doi:10.1080/10826070903091712

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…The injection volume capacity was 10 μL. The separation of GLZ was executed using “Acquity UPLC BEH C18 column (2.1 × 50 mm 2 , 1.7 μm, Waters)” maintained at T = 303.2 ± 1 K. The proposed UPLC-UV technique was used to quantify GLZ with some modifications in the reported HPLC-UV methods. − In the proposed analytical methodology, the mobile phase (composed of 80:20, v/v methanol and 10 mM KH 2 PO 4 buffer of pH 4.2 adjusted with orthophosphoric acid) was flowed with an isocratic mode. The flow rate and injection volume were set at 0.08 mL min –1 and 5 μL, respectively.…”

Section: Methodsmentioning

confidence: 99%

Solubility Measurement and Various Solubility Parameters of Glipizide in Different Neat Solvents

et al. 2020

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Glipizide (GLZ) is an oral hypoglycemic agent, which is a weakly aqueous soluble drug. The solubility values of GLZ in various neat solvents are scarce in the literature. Hence, the solubility of GLZ in 12 different neat solvents, namely, "water, methanol, ethanol, isopropanol (IPA), 1-butanol, 2-butanol, ethylene glycol (EG), propylene glycol (PG), poly(ethylene glycol)-400 (PEG-400), ethyl acetate (EA), dimethyl sulfoxide (DMSO), and Transcutol-HP (THP)", at "T = 298.2−318.2 K" and "p = 0.1 MPa" was measured. The recorded solubilities of GLZ were correlated by "van't Hoff and Apelblat models" using rootmean-square deviation (RMSD). The overall RMSD was obtained as 1.21 and 1.40% for "Apelblat and van't Hoff models", respectively. Different solubility parameters of all studied materials including drug and solvent were calculated to find the best solvent for GLZ. The solubilities of GLZ (expressed in mole fraction) have been found highest in DMSO (2.81 × 10 −2 ), followed by THP, EA, 2-butanol, 1-butanol, IPA, PEG-400, ethanol, PG, methanol, EG, and water (1.98 × 10 −4 ) at "T = 318.2 K". All investigated solubility parameters of GLZ were recorded very close to the DMSO. "Apparent thermodynamic analysis" showed an "endothermic and entropy-driven dissolution" of GLZ in the 12 different neat solvents. The highest molecular interactions were recorded in GLZ−DMSO compared to other combinations. Overall, DMSO has been considered as the best solvent for the solubilization of GLZ.

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Section: Methodsmentioning

confidence: 99%

Solubility Measurement and Various Solubility Parameters of Glipizide in Different Neat Solvents

et al. 2020

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“…Extraction and analysis of the drug from blood samples 19 Five hundred microliters of plasma or calibration standards, 50 µL of internal standard solution (10 mcg/ml glibenclamide), and 850 ml of 0.05M HCl were added to a glass tube. After mixing using and MS2 Minishaker (IKA ® Works, INC., Wilmington, NC, USA), 5 ml of diethyl ether was added and the mixture was stirred for 30 seconds.…”

Section: Blood Samplingmentioning

confidence: 99%

Formulation and Evaluation of Buccoadhesive Sustained-Release Discs of Glipizide

Aboutaleb

Abdel‐Rahman

Khedr

et al. 2017

Bulletin of Pharmaceutical Sciences. Assiut

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Glipizide is an oral hypoglycemic agent used in the treatment of type II diabetes mellitus. It is characterized by its poor aqueous solubility and delayed absorption with concomitant food intake. The objective of the present study was to enhance the absorption rate of glipizide and avoid its side effects on stomach by formulating it into buccoadhesive sustained release disc formulations. The discs were prepared by direct compression method. Hydroxypropyl methylcellulose (HPMC 15000), was used as the main hydrophilic matrix forming polymer either alone or in combinations in two ratios (3:2 & 4:1) with various mucoadhesive polymers namely; Sodium alginate (NaAlg), Sodium carboxymethyl cellulose (SCMC), Hydroxyethyl cellulose (HEC), Hydroxypropyl cellulose (HPC) and Chitosan. The discs were evaluated for weight variation test, thickness, diameter, drug content, hardness, friability, swelling index, surface pH, in-vitro bioadhesion, in-vitro release studies and in-vivo bioavailability studies. Invitro release studies demonstrated that formulation F8 which contains HPMC / SCMC (40%: 10%) has sustained the drug release up to 8 hrs which was considered an optimum pattern of drug release. The kinetic studies revealed that all formulations follows zero order release kinetics except F3, F4 and F11 which fitted well in first order release model. Bioavailability parameters including C max , T max , and AUC 0-24 h of F8 and the commercial oral tablets of glipizide (Minidiab ® 5 mg) were compared. The selected formulation F8 produced higher C max and extended T max (P<0.05).

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“…Owing to its therapeutic importance different methods are available for determination of GPZ in body fluids and include, HPLC [3][4][5][6][7][8][9][10][11], LC-MS/MS [12][13][14][15][16], UPLC-MS/MS [17,18] and radioimmunoassay [19]. European Pharmacopeia [20] describes a titrimetric assay in which drug in dimethyl formamide is titrated with lithium methoxide using quinaldine red as indicator.…”

Section: Glipizidementioning

confidence: 99%

Rapid and Reliable Determination of Glipizide in Pharmaceutical Samples by HPLC and Its Degradation Study

Basavaiah¹

2017

Austin J Anal Pharm Chem

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A simple, specific and rapid high-performance liquid chromatographic (HPLC) method for the determination of glipizide (GPZ) in pharmaceutical sample is described. Reversed phase chromatography was performed on an Inertsil ODS 3V (150 × 4.6mm; 5µm particle size) column with an isocratic mobile phase consisting of 10mM potassium dihydrogen phosphate (pH 3.9) and methanol (60:40 v/v). The effluent was monitored on a uv detector at 220nm and the column temperature was maintained at 35ᴼC. Linear response (r=0.9999) was observed over the range, 1-450µgmL -1 ; and the limits of detection (LOD) and quantification (LOQ) were calculated to be 0.03 and 0.09 µgmL -1 , respectively. Both intra-day and inter-day precisions at three tested concentrations were excellent, with %RSD values of <1% and the accuracy was better than 1.5% (RE). The method was also validated for robustness, ruggedness and selectivity and the results were satisfactory. The method was applied to the determination of GPZ in tablets and the results agreed well with the label claim and those obtained by European Pharmacopeial method. Entire assay was complete in less than 10min. As part of degradation study, the drug was subjected to acid-, base-, peroxide-, heat-, and light-induced stress conditions, and the drug was found to be susceptible to degradation under oxidation, and inert to other conditions.

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HPLC Analysis of Plasma Glipizide and its Application to Pharmacokinetic Study

Cited by 11 publications

References 12 publications

Solubility Measurement and Various Solubility Parameters of Glipizide in Different Neat Solvents

Solubility Measurement and Various Solubility Parameters of Glipizide in Different Neat Solvents

Formulation and Evaluation of Buccoadhesive Sustained-Release Discs of Glipizide

Rapid and Reliable Determination of Glipizide in Pharmaceutical Samples by HPLC and Its Degradation Study

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