HPLC Analysis of Phenolic Acids and Antioxidant Activity of Some Classical Ayurvedic Guggulu Formulations

Hazra, Alok Kumar; Sur, Tapas Kumar; Chakraborty, Banti; Seal, Tapan

doi:10.7897/2277-4343.09122

Cited by 6 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These are sub-classified as benzoic acid and cinnamic acid backbone structure containing seven (C6-C1) and nine (C6-C3) carbon atoms, respectively. Hydroxybenzoic acid derivatives such as gallic acid, syringic acid and cinnamic acid derivatives like caffeic acid, chlorogenic acid, sinapic acid, p-coumaric acid are widely available in plants 33 , 34 , 59 , 60 . Masek et al ., concluded that caffeic acid is the predominantly and widely available phenolic acid, and it exhibits antioxidant and iron-chelating abilities which are better than p coumaric acid 61 .…”

Section: Discussionmentioning

confidence: 99%

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

et al. 2020

View full text Add to dashboard Cite

Background: Medicinal plants are a source of phytochemicals and they are used for the treatment of several oxidative stress-related or other diseases for their effectiveness, low toxicity and easy availability. Five traditionally used and less characterized herbaceous weeds of West Bengal, India, namely, Heliotropium indicum, Tridax procumbens, Cleome rutidosperma, Commelina benghalensis and Euphorbia hirta, were investigated for the current research study. Methods: Aqueous and 70% ethanolic extracts of the leaves were analyzed for estimation of essential phytochemicals and to evaluate their in vitro antioxidant status, medicinal properties and cytotoxic effects. To the best of our knowledge, several assays and comparative evaluations using these herbs are reported for the first time. For quantitative study, UV-vis spectrophotometry and high-performance liquid chromatography with diode array detector HPLC-DAD techniques were used. Antibacterial properties were investigated using the Kirby-Bauer disc diffusion method. For in vitro anti-lithiatic study, a titration method was used. The cell viability assay was done using peripheral blood mononuclear cells. Results: The aqueous extract exhibits higher content of polyphenols, flavonoids, tannins and inhibition percentage values for free radical scavenging assays, whereas the 70% ethanolic extract exhibits higher content of alkaloids and cardiac glycosides. HPLC-DAD analysis of 70% ethanolic extracts led us to identify 10 predominant phenolic constituents. Euphorbia hirta extracts showed minimum cytotoxicity (cell death ~2.5% and 4% in water and 70% ethanolic extract, respectively), whereas Cleome rutidosperma and Tridax procumbens’ 70% ethanolic extracts showed higher cell death (~13% and 28%, respectively), compared with the control (cell death ~10-12%). Conclusions: The study concluded that of all the medicinal weeds selected for the current study, Euphorbia hirta possesses the highest amount of bioactive compounds and hence exhibits the highest in vitro antioxidant activity and promising in vitro medicinal properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Among the different phenolic acids and flavonols, OC contains a higher amount of Gallic acid and Kaempferol with respect to OS, as evident from chromatograms. Like other polyphenols, Gallic acid and Kaempferol can help to cure oxidative stressrelated diseases like diabetes and microbial pathogenesis [2]. They have anticancer activity.…”

Section: Fig 16 Overall Work Flow Of the Current Research Workmentioning

confidence: 99%

“…They contain a diverse range of chemical compounds of high therapeutic value. These phytochemicals such as polyphenols, flavonoids, tannins, alkaloids, and other organic components belongs to secondary metabolites of medicinal plants [1][2][3][4][5][6]. These compounds have a vital function in protecting us against infections and other diseases.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profiling and Pharmacognostic Evaluation of Oldenlandia corymbosa and Ocimum sanctum Leaves Hydroalcoholic Extracts: Comparative Study

Das

Ghosh²,

Ghosh

et al. 2022

JPRI

View full text Add to dashboard Cite

Herbs are an important source of bioactive substances. These are widely used to treat several disorders for better function in the human body, minimum toxic effects, and widespread availability. A total of two medicinal herbs from West Bengal, India, such as Oldenlandia corymbosa (Diamond flower) and Ocimum sanctum (Holy basil), are being considered for inclusion in the current study. Hydroalcoholic extracts (70% ethanolic) of the two plants' leaves were analyzed to detect and quantify important phytochemical substances and investigate in vitro antioxidant and pharmacological effects. Spectrophotometric and HPLC-DAD techniques were used for the quantitative estimation of different phytochemicals. In addition, in vitro antimicrobial properties were studied using the Kirby-Bauer paper disc diffusion method. Several assays have been performed on the medicinal plant Oldenlandia corymbosa (OC). The results have been compared to those obtained from a traditional medical plant, Ocimum sanctum (OS) for the first time to our knowledge. Results showed that OS contains a higher quantity of polyphenols, flavonoids, and has higher antioxidant potential with respect to OC. Similar trends were observed for polysaccharides contents. In contrast, OC contains a higher quantity of tannins, alkaloids, and protein and higher in vitro antibacterial and anti-diabetic properties. HPLC-DAD-based profiling of eight important phenolic constituents viz. Gallic acid, catechin hydrate, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, sinapic acid, coumarin, quercetin, and kaempferol, were performed. The current study concluded that Oldenlandia corymbosa has many bioactive phenolics in considerable amounts compared to the highly established medicinal herb OS leaves extracts. The current study demonstrates the pharmacological significance of Oldenlandia corymbosa that may generate enthusiasm among researchers and those working in the pharmaceutical industry.

show abstract

“…Kwas elagowy występował w produktach na poziomie około 5 mg/g, a kwas syringowy 2,5 mg/g. Pozostałe związki stwierdzono w znacznie niższym stężeniu (poniżej 1 mg/g produktu) [15].…”

Section: Kwasy Fenoloweunclassified

Guggul- a herbal panacea from India

Wojciechowska¹,

Grzegorczyk-Karolak²,

Staniewska³

2020

Farm Pol.

View full text Add to dashboard Cite

Guggul-a herbal panacea from India • Guggul is an oleo-gum resin obtained from plants belonging to the genera Commiphora and Boswellia, within the Burseraceae family. Its source are mainly two species: Commiphora wightii (Arn.) Bhand. (syn. Commiphora mukul (Hook. ex Stocks) Engl. and Boswellia serrata (Roxb. ex Colebr.), which are native to India. In this area, they have been known from centuries and used in the traditional Indian medicine-Ayurveda for the treatment of variety of disorders such as: rheumatism, inflammation, liver dysfunction, gout, obesity, diabetes and disorders of lipids metabolism. The oleo-gum occurs in the resin canals located in the larger veins of the leaf and in the soft base of the stem. It is obtained through incision of the tree bark and outflow of the viscous, golden substance with characteristic myrrh-like fragrance. Guggul derived from C. wightii is a mixture of various chemical compounds, namely: an essential oil consisting of monoterpenes and sesquiterpenes; as well as diterpenes, triterpenes, steroids, phenolic acids, lignans, guggultetrols, carbohydrates and amino acids. Whereas, the raw material obtained from B. serrata consists of resin, which is mostly made up of tetraand pentacyclic triterpene acids, and moreover a hydrophilic fraction and an essential oil being a mixture of terpenes. It has been proven that guggul exerts various biological activities and influences life functions through effects on lipids and glucose metabolism, endogenous nuclear receptors, inflammatory mediators or thyroid hormone homeostasis. It also has valuable antineoplastic, antimicrobial, hepato-, neuro-and cardioprotective properties. Nowadays, when the majority of the society takes notice to the healthy lifestyle and condition of natural environment, guggul and its components are increasingly used as dietary supplements. The following review summarizes the knowledge on the sources, phytochemistry, traditional and modern use, pharmacological properties and toxicological data of the raw material derived from C. wightii and B. serrata.

show abstract

HPLC Analysis of Phenolic Acids and Antioxidant Activity of Some Classical Ayurvedic Guggulu Formulations

Cited by 6 publications

References 12 publications

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

Phytochemical Profiling and Pharmacognostic Evaluation of Oldenlandia corymbosa and Ocimum sanctum Leaves Hydroalcoholic Extracts: Comparative Study

Guggul- a herbal panacea from India

Contact Info

Product

Resources

About