HPLC analysis of human erythrocytic glutathione forms using OPA and N-acetyl-cysteine ethyl ester: Evidence for nitrite-induced GSH oxidation to GSSG

Optimized Method for Determining FreeL-Cysteine in Rat Plasma by High-Performance Liquid Chromatography with the 4-Aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole Conversion Reagent

“…7) Among these, methods using HPLC with fluorescence detection are the most popular because of their high sensitivity and reasonable simplicity. The thiols in a sample are detected by converting with such fluorescence reagents as ortho-phthalaldehyde (OPA), 8) monobromobimane (mBBr), 9) N-(1-pyrenyl)-maleimide (NPM), 10) and benzofurazan reagents including 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) 11) and 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F).…”

Microbeam-coupled capillary electrophoresis

“…(14) Nitric oxide (NO † ) DAF-FM 495 515 (13) Hydroxyl (OH † ) TA 315 425 (12) 'General' ROS DHR123, DHE, MitoSOX 505 500 525 600 (9 -11) Glutathione (GSH, GSSG) NDA, OPA 430 338 530 458 (18,19) Figure 1. Fluorescence spectra measured using the RARAF breadboard LIF system of DHR-123 before (grey) and after (black) addition of KO 2 , a superoxide generator.…”

A Fluorescent-Based HPLC Assay Using 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole as Derivatization Agent for the Determination of Iron Bioavailability to Red Tide Phytoplankton

“…These fluorescent reagents were reported previously to be useful for the fluorometric detection of proteins, peptides, amino-and imino-acid [24][25][26]. A 10 µL extract sample of each treatment was added to 100 µL of 0.266 M NaHCO 3 and 80 µL of 4.75 mg mL -1 NBD-Cl/MeOH.…”

“…Fluorescent-based HPLC assay is a quick, easy, efficient and powerful method for the quantitative measurement of cellular macromolecules in organisms [22][23][24][25][26][27]. This method has been used for the determination of cysteine and cysteamine adducts in Escherichia coli-derived proteins [26], thiol compounds in biological samples [22], human erythrocytic glutathione [25], domoic acid (DA) in mussels (Mytilus edulis) [24], peptides in tissue and plasma samples [28] and amines and proteins [29] using different fluorescent reagents as the derivatizer.…”

“…This method has been used for the determination of cysteine and cysteamine adducts in Escherichia coli-derived proteins [26], thiol compounds in biological samples [22], human erythrocytic glutathione [25], domoic acid (DA) in mussels (Mytilus edulis) [24], peptides in tissue and plasma samples [28] and amines and proteins [29] using different fluorescent reagents as the derivatizer. The HPLC method has also been used for the determination of pigments in phytoplankton in order to measure marine productivity in relation of Fe status in seawater [30,31].…”