HPLC analysis of blood–brain barrier penetration of 4-fluorodeprenyl

Abstract

Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30 mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60 min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24 μg/g, showing maximum concentration in the brain and the liver after 5 min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15 min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.

Pharmacokinetics of Selegiline in a Rabbit Model

“…The flow rate of the mobile phase was 1 ml/min, the column was kept at 35 °C and the injection volume was 75 µl. Conditions of sample treatments and validation of HPLC determinations are detailed in our recent publications [30,34]. HPLC of rabbit iris samples is given in Fig.…”