HPLC analysis of bioactive flavonoids from the rhizome of Alpinia officinarum

Lin, Tao; Wang, Z.-T.; Zhu, Enyuan; Lü, Yanhua; Wei, Dongzhi

doi:10.1016/j.sajb.2005.06.007

Cited by 39 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nowadays, it is cultivated in several places around the world due to the attractive beauty of its inflorescences and its therapeutic potential (Soares de Moura et al, 2005;Victório, 2008). In addition, these plants are important sources of raw material for many useful products: foods, spices, medicines, perfumes, dyes and fiber paper (Tomlinson, 1969).…”

Section: Introductionmentioning

confidence: 99%

Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography

Victório

Kuster

Lage

2009

Rev. bras. plantas med.

View full text Add to dashboard Cite

ABSTRACT:The species Alpinia purpurata is scarcely cited as to ethnopharmacology and phytochemistry. This study aimed to analyze bioactive compounds through high-performance liquid chromatography (HPLC). Hydroalcoholic crude extract was obtained from A. purpurata dried leaves. Folin-Ciocalteau method was used to quantify total phenols, using gallic acid as standard. The obtained result was 15.6 mg GAE g -1 . The crude extract was partitioned with the solvents ethyl acetate and butanol, followed by thin-layer chromatography (TLC) and HPLC. The flavonoids kaempferol-3-O-glucuronide and rutin were detected at a higher concentration in ethyl acetate and butanolic extracts. The butanolic extract contains the highest flavonoid percentage (94.3%). A. purpurata presents important flavonoids of therapeutic use, already verified for A. zerumbet. This is the first study verifying the presence of flavonoids in A. purpurata extracts.Key words: TLC, polyphenols, medicinal plants, rutin, Zingiberaceae RESUMO: Detecção de flavonóides em folhas de Alpinia purpurata (Vieill.) K. Schum. por cromatografia líquida de alta eficiência. A espécie Alpinia purpurata apresenta poucas citações referentes a etnofarmacologia e fitoquímica. Este estudo propõe a análise de substâncias bioativas através da técnica de cromatografia líquida de alta eficiência (CLAE). O extrato bruto hidroalcóolico foi obtido a partir de folhas secas de A. purpurata. A quantificação de fenóis totais foi realizada pelo método de Folin-Ciocalteau, usando ácido gálico como padrão. Como resultado, foi verificado 15,6 mg EAG g -1 . O extrato bruto foi particionado com os solventes acetato de etila e butanol e depois analisado por cromatografia em camada delgada e CLAE. Nos extratos acetato de etila e butanólico foi detectada a presença dos flavonóides kaempferol-3-O-glicuronídeo e rutina, em maior concentração. O extrato butanólico contém a maior porcentagem de flavonóides (94,3%). Esta espécie possui flavonóides importantes no uso terapêutico, já antes verificados para a espécie A. zerumbet. Este é o primeiro trabalho que verifica a presença de flavonóides em extratos de A. purpurata.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography

Victório

Kuster

Lage

2009

Rev. bras. plantas med.

View full text Add to dashboard Cite

show abstract

“…Reversed-phase HPLC is one of the most employed techniques for the analysis of flavonoids [17]. Some results using HPLC were reported for Alpinia officinarum and A. purpurata species [1,19]. The described HPLC procedures can be useful for the qualitative and quantitative analysis of flavonoids in crude extracts, especially from Zingiberaceae family that has a pronounced presence of flavonoids in its species verified through chemotaxonomic studies [20].…”

Section: Resultsmentioning

confidence: 99%

Flavonoids extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different procedures

2010

View full text Add to dashboard Cite

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversed-phase HPLC techniques the rutin and kaempferol-3-O-glucuronide were detected. Ethanol 70% was more efficient for flavonoids extraction than water. No significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). Relative concentration of rutin and kaempferol-3-Oglucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g-1 dried leaves) and by microwave (1.0 and 6.64 mg g-1 dried leaves) methods using 70% ethanol. Rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.

show abstract

“…This mean 95.4% antiradical activities of Zingiberaceae family extracts were donated by phenolic compounds and as many as 4.6% activities were donated by other compounds. Phenolic compound contained in Zingiberaceae family are gingerol, shogaol, gingeron (Ravindran, et al, 2005), bisdescurcuminoid (Wulandari, 1996), kaempferol (Usia, et al, 2004), flavonoid and non polar constituents such as curcuminoid, kavapiron and gingerol (Sirat, et al, 1996), galangin, kaempferid and pinobanksin flavononol (Hisashi, et al, 2005), 3-O-metil galangin (Tao, et al, 2006).…”

Section: Correlation Between Radical Scavenging Activity and Total Phmentioning

confidence: 99%

Potency of Radical Scavenging Activity and Determination of Total Phenolic Content of Five Ethanolic Extract of Rhizome Zingiberaceae Family

Da'i

Setiawan

Melannisa

2013

Indones J Cancer Chemoprevent

View full text Add to dashboard Cite

Several studies show that some plants and fruits could protect human body from free radical danger exposure. Zingiberaceae family has some chemical substances that have antiradical activity, such as phenolic compound and flavonoid. The aim of this study is to determine the correlation of total phenolic compound towards its antiradical activity. Antiradical activity assay was determined by DPPH method (1,1 difenil-2-pikrilhidrazil). The total phenolic compound is determined by spectrofotometry using Folin-Ciocalteu reagent and counted as GAE (Gallic Acid Equivalent). Based on this study, the total phenolic concentration of ethanolic extract of jahe (Zingiber officinale (L.) Rosc), kencur (Kaemferia galanga L.), lengkuas (Languas galanga), lempuyang gajah (Zingiber littorale) and lempuyang pahit (Zingiber americana) is 73.99 mg/g; 23.848 mg/g; 37.841 mg/g; 49.725 mg/g and 40.802 mg/g respectively. The radical scavenging activity (IC50) of jahe (27.38 µg/mL), lengkuas (93,27 µg/mL), lempuyang pahit (105,08 µg/mL), meanwhile lempuyang gajah and kencur show low activity of radical scavenging.

show abstract

HPLC analysis of bioactive flavonoids from the rhizome of Alpinia officinarum

Cited by 39 publications

References 10 publications

Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography

Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography

Flavonoids extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different procedures

Potency of Radical Scavenging Activity and Determination of Total Phenolic Content of Five Ethanolic Extract of Rhizome Zingiberaceae Family

Contact Info

Product

Resources

About