HPLC Analysis of Allicin and Other Thiosulfinates in Garlic Clove Homogenates

Lawson, Larry D.; Wood, Steve; Hughes, Bronwyn G.

doi:10.1055/s-2006-960087

Cited by 180 publications

(171 citation statements)

References 12 publications

(17 reference statements)

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“…(C. albicans, C. glabrata, C. parapsilosis, C. tropicalis and C. crusei. 7 In spite of geographic variation, 'garlic extract liquid' for our isolates are consistent with those of Sivam, 8 but are relatively lower than values obtained in the literature. 9 This antimicrobial potency disparity of garlic has been attributed to the different concentrations of individually and synergistically active biosubstances in garlic preparations coupled with their interactions with sulfhydryl agents in culture media.…”

Section: Pointsupporting

confidence: 83%

Comparative Clinical Study of the Effectiveness of MEKRITEN in Patients with Chronic Suppurative Otitis

Mukhitdinov

Olimov

Olimova

2018

tjps

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Objectives: Chronic suppurative otitis media (CSOM) is a serious disease with the presence of bacterial infection in the middle ear. Worldwide, 1% to 46% of the population living in developed and developing countries have CSOM, about 65-330 million people, 60% of whom have significant hearing loss. The study of the wound healing efficacy and tolerability of the drug MEKRITEN (garlic extract liquid) was performed to identify the possibility of issuing recommendations for the drug for clinical use in the Republic of Uzbekistan. Materials and Methods:The main group of patients, who received the study drug, consisted of 30 patients; the group of patients who received the comparison drug comprised 20 patients. Patients of the main group (30 persons) were given 'garlic extract liquid,' which was developed by the Tashkent Pharmaceutical Institute, given as 2 drops twice per day for 10 days in the external auditory canal. Patients in the comparison group (20 people) took other drugs (0.25% solution of levomycetin) in a similar way. Patients were examined and observed carefully to determine the severity of symptoms such as pain, secretion (in points), and clinical analyses were performed including general blood analysis, and urine. In addition, the researchers performed biochemical (ALT, AST, bilirubin), instrumental (ultrasound of the liver), and special (biopsy of fibrous tissue of echinococcal capsules) analyses. Results: The average rating of the tolerability and effectiveness in the points for MEKRITEN made portability of 4.97 points, and the effectiveness was 4.77 points, and for 0.25% levomycetin solution, they were 4.8 and 3.35 points, respectively. While receiving MEKRITEN, no adverse effects and allergic reactions were observed. No criteria for treatment cessation were observed (adverse effects, ineffective treatment, poor adherence to protocol, and refusal to continue) thus the study was not stopped in any patients. Conclusion: Local therapy with the drug MEKRITEN in patients with CSOM is more effective than local application of 0.25% levomycetin solution. Local therapy with MEKRITEN in the treatment of CSOM leads to faster termination of otorrhoea and is accompanied by fewer adverse drug reactions. Key words: MEKRITEN, levomycetin, chronic suppurative otitis media, garlic extract liquid Amaç: Kronik süpüratif otitis media (KSOM), orta kulakta bakteri enfeksiyonu bulunan ciddi bir hastalıktır. Dünya çapında, gelişmiş ve gelişmekte olan ülkelerde yaşayan nüfusun %1-46'sı, yaklaşık 65-330 milyon insan, KSOM'dan muzdarip olup, bunların %60'ında ise önemli işitme kaybı vardır. Özbekistan Cumhuriyeti'nde klinik kullanım için ilaç önerileri verme olasılığını belirlemek için MEKRITEN (sarımsak özü sıvısı) ilacın yara iyileşme etkinliği ve tolere edilebilirliği araştırılmıştır. Gereç ve Yöntemler: Çalışma ilacını alan ana grup 30 hastadan, karşılaştırma ilacı alan hasta grubu 20 hastadan oluşmaktadır. Ana grubun (30 kişilik) hastalarına, Taşkent İlaç Enstitüsü tarafından geliştirilen sarımsak özü sıvısı 10 gün...

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Section: Pointsupporting

confidence: 83%

Comparative Clinical Study of the Effectiveness of MEKRITEN in Patients with Chronic Suppurative Otitis

Mukhitdinov

Olimov

Olimova

2018

tjps

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“…(2-propenyl E-1-propenethiosulfinate; 2P1PTS) and 2-propene-1-sulfinothioic acid S-(E,Z)-1-propenyl ester ((E,Z)-1-propenyl 2-propenethiosulfinate; 1P2PTS) by comparison of the obtained NMR and MS data with that previously reported (Lawson et al, 1991;Block et al, 1996;Calvey et al, 1997;Yoshida et al, 1999). Both compounds are known to be present in garlic homogenates (Lawson et al, 1991;Block et al, 1992).…”

Section: Resultsmentioning

confidence: 74%

“…Reportedly, in unheated garlic puree the concentrations of 2P1PTS and 1P2PTS are almost equal (Lawson et al, 1991). The reason for the synergistic effect observed in color developing activity, (0.5-2P1PTS + 0.5-1P2PTS) > 2P1PTS > 1P2PTS, has not yet been elucidated.…”

Section: Resultsmentioning

confidence: 97%

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Isolation and Identification of Causal Constituents of Green Discoloration in Garlic Puree

Yamazaki

Takada

Okuno

2012

FSTR

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The green discoloration of garlic (Allium sativum L.) puree, a serious problem in garlic processing, was investigated. Two thiosulfinates, 2-propenyl E-1-propenethiosulfinate (2P1PTS) and (E/Z)-1-propenyl 2-propenethiosulfinate (1P2PTS), were isolated from unheated garlic puree (homogenate), and were confirmed to be the direct causal constituents ('color developer') of green discoloration based on a color developing assay. 2P1PTS and/or 1P2PTS were reacted with glycine to develop a blue-green color similar to the discoloration of garlic purees. 2P1PTS and 1P2PTS showed synergy for greening; the color developing activities of three solutions, 2P1PTS, 1P2PTS and an equimolar mixture of them (0.5-2P1PTS + 0.5-1P2PTS; similar to the natural abundance ratio) were in the rank order: (0.5-2P1PTS + 0.5-1P2PTS) > 2P1PTS > 1P2PTS.

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“…Allicin was isolated from garlic extract using preparative silica gel thin layer chromatography using toluene:ethyl acetate (100:30) as developing solvent then identified by spectroscopic data. The resulting spectra were compared with reported data (Cruz-Villalon, 2001;Lawson et al, 1991;Jansen et al, 1987). Pure compound was used to prepare calibration curve.…”

Section: Determination Of Allicin Contentmentioning

confidence: 99%

Studies on garlic production in Egypt using conventional and organic agricultural conditions

Raslan¹,

AbouZid²,

Abdallah³

et al. 2015

Afr. J. Agric. Res.

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Balady and Chinese-Sids40 are the main garlic cultivars grown in Egypt. Effect of conventional vs. organic agricultural practices on premature and mature garlic bulb yield was studied for these cultivars. Organic agriculture increased yield of garlic bulbs and was more effective in Balady than in ChineseSids40 cultivar. Effect of cultivar type, maturity stage and agricultural methods on allicin content in garlic bulbs was studied using high performance liquid chromatography. Cultivar type and agricultural methods showed more difference in allicin content in cured mature bulb stage than in fresh premature bulb stage. In case of cured mature bulbs, Chinese-Sids40 had higher allicin content (3.02 mg/g FW) than the Balady (2.59 mg/g FW). Effect of storage temperature on allicin content in conventionally-and organically-grown garlic bulbs was studied. Allicin content was higher in organically-grown than conventionally-grown garlic stored at room temperature after six months. At 0°C, allicin content was higher in conventionally-grown than organically-grown garlic. The decrease in allicin content can be explained by the prolonged dormancy period and hindered sprouting that suppressed the metabolic activity.

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HPLC Analysis of Allicin and Other Thiosulfinates in Garlic Clove Homogenates

Cited by 180 publications

References 12 publications

Comparative Clinical Study of the Effectiveness of MEKRITEN in Patients with Chronic Suppurative Otitis

Comparative Clinical Study of the Effectiveness of MEKRITEN in Patients with Chronic Suppurative Otitis

Isolation and Identification of Causal Constituents of Green Discoloration in Garlic Puree

Studies on garlic production in Egypt using conventional and organic agricultural conditions

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