HPLC Analysis of 6-Mercaptopurine and Metabolites in Extracellular Body Fluids

Rudy, J L; Argyle, J. Craig; Winick, Naomi J.; Dreal, P Van

doi:10.1177/000456328802500505

Cited by 17 publications

(6 citation statements)

References 12 publications

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“…A few methods for determination of both or one or the other compounds in human urine by HPLC with UV detection were described [106][107][108][109]. Almost all methods [106][107][108] make use of natural structural properties of thiopurines, which possess a well-defined absorption maximum in the ultraviolet spectrum. Recently, Zakrzewski [110] reported a post-column reaction detection method for HPLC determination of thioguanine, based on the sensitizing induction of thioguanine on iodine-azide reaction.…”

Section: Thiopurinesmentioning

confidence: 99%

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

Kuśmierek

Chwatko

Głowacki

et al. 2009

Journal of Chromatography B

124

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Section: Thiopurinesmentioning

confidence: 99%

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

Kuśmierek

Chwatko

Głowacki

et al. 2009

Journal of Chromatography B

124

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“…The ideal method should allow the assessment of patient compliance with the treatment, together with measurement of the absorption, transport and accumulation of the cytotoxic metabolites (31). Studies concerning the quantification of 6-MP and its metabolites in the urine of patients showed that the main disadvantage of this type of sample is that it can detect failures in adherence to the treatment only when the analysis is performed shortly after the missed event (6).…”

Section: Discussionmentioning

confidence: 99%

An improved HPLC method for the quantitation of 6-mercaptopurine and its metabolites in red blood cells

Oliveira

Romanha

Alves

et al. 2004

Braz J Med Biol Res

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A procedure is described for the rapid determination of the intraerythrocyte concentration of 6-mercaptopurine (6-MP) and its metabolites, 6-thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine (6-MMP). Erythrocytes (8 x 10 8 cells) in 350 µl Hanks solution containing 7.5 mg dithiothreitol were treated with 50 µl 70% perchloric acid. The precipitate was removed by centrifugation (13,000 g) and the supernatant hydrolyzed at 100°C for 45 min. After cooling, 100 µl was analyzed directly by HPLC using a Radialpack Resolve C18 column eluted with methanol-water (7.5:92.5, v/v) containing 100 mM triethylamine. 6-TG, 6-MP and the hydrolysis product of 6-MMP, 4-amino-5-(methylthio)carbonyl imidazole, were monitored at 342, 322 and 303 nm using a Shimadzu SPD-M10A diode array UV detector. The analytes eluted at 5.3, 6.0 and 10.2 min, respectively. The calibration curves were linear (r 2 > 0.998), and the analytical recoveries were 73.2% for 6-TG, 119.1% for 6-MP and 97.4% for 6-MMP. The intra-and inter-assay variations were highest for 6-MP (9

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“…Since 6-MP is widely used in chemotherapy, it is important to develop and validate analytical methods for its determination in pharmaceutical dosage forms. Review of literature has revealed that all methods reported for the estimation of 6-MP are useful in estimating 6-MP in biological samples (Sorouraddin et al, 2011;Hawwa et al, 2009;Umrethia et al, 2006;Oliveira et al, 2004;Su et al, 1999;Mawatari et al, 1998;Sahnoun et al, 1990;Erdmann, Chan, Canafax, 1988;Rudy et al, 1988;Migulla, Prumke, Huller, 1987). So far no systematic HPLC method has been reported for determination of 6-MP in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Optimization and validation of an RP-HPLC method for the estimation of 6-mercaptopurine in bulk and pharmaceutical formulations

Somasekhar¹

2014

Braz. J. Pharm. Sci.

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A reverse phase HPLC method is described for the determination of 6-mercaptopurine in bulk and tablets. Chromatography was carried on a C 18 column using a mixture of acetonitrile and 0.05 mol/L sodium acetate buffer (10:90 v/v) as the mobile phase at a flow rate of 1 mL/min -1 with detection at 324 nm. The retention time of the drug was 3.25 min. The detector response was linear in the concentration of 0.01-5 μg/mL. The limit of detection and limit of quantification were 17 and 52 ng/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of mercaptopurine in bulk and tablets.Uniterms: 6-Mercaptopurine/determination. High perfomance liquid chromatography/reverse phase/ quantitative analysis. Pharmaceutical formulations/quality control Descreve-se método de CLAE em fase reversa para a determinação de mercaptopurina a granel e em comprimidos. A cromatografia foi realizada em coluna C18, utilizando mistura de acetonitrila em tampão acetato de sódio 0,05 mol/L (10:90 v/v) como fase móvel, com fluxo de 1 mL/min e detecção a 324 nm. O tempo de retenção do fármaco foi de 3,25 min. A resposta do detector foi linear na concentração de 0,01-5 μg/mL. O limite de detecção e o limite de quantificação foram de 17e 52 ng/mL, respectivamente. O método foi validado pela determinação de sua sensibilidade, linearidade, acurácia e precisão. O método proposto é simples, econômico, rápido, acurado e preciso e, então, pode ser aplicado para controle de qualidade de rotina da mercaptopurina em batelada e em comprimidos.Unitermos: Mercaptopurina/determinação. Cromatografia líquida de alta eficiência/fase reversa/análise quantitativa. Formulações farmacêuticas/controle de qualidade.

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HPLC Analysis of 6-Mercaptopurine and Metabolites in Extracellular Body Fluids

Cited by 17 publications

References 12 publications

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

An improved HPLC method for the quantitation of 6-mercaptopurine and its metabolites in red blood cells

Optimization and validation of an RP-HPLC method for the estimation of 6-mercaptopurine in bulk and pharmaceutical formulations

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