HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts

Abstract

A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.

Ultrasound-Assisted Extraction of Verbascoside from Clerodendrum glandulosum Leaves for Analysis of Antioxidant and Antidiabetic Activities

“…Qualitative and quantitative analyses of polyphenols were conducted in a Waters Breeze QS manual injector HPLC system (Waters Corporation, Milford, Massachusetts, USA) equipped with Waters 1525 binary HPLC pumps connected to a Waters 2998 photodiode-array detector (PDA) and the Breeze software. Chromatographic analyses were carried out using a C18 column (80 Å, 5 μm, 4.6 × 250 mm) at 25 °C using 1% acetic acid in water (A) and methanol (B) as a mobile phase . The flow rate was maintained at 1 mL/min, and 20 μL of the sample was injected.…”

“…Chromatographic analyses were carried out using a C18 column (80 Å, 5 μm, 4.6 × 250 mm) at 25 °C using 1% acetic acid in water (A) and methanol (B) as a mobile phase. 21 The flow rate was maintained at 1 mL/min, and 20 μL of the sample was injected. Extracts were investigated for VER at 335 nm wavelength.…”

“…A total of 162.11 mg/g (i.e., 16.211%) of VER contents were reported from ethanolic extracts of Abeliophyllum distichum. 21 The retention time, UV spectra, and co-chromatography of the peak were all consistent with the VER standard. The chemical identity of VER was further confirmed by HPTLC, LC−MS, and NMR.…”

Effects of Different Drying Methods on the Contents of Nine Components and Immunomodulatory Activities of Four Components in Osmamthus fragrans Flowers

“…7 Phenylpropanoids have antioxidant, anti-melanogenesis, antihypoxia, anti-inflammatory, anti-aging, and other biological activities. [8][9][10][11][12] Six flavonoids were isolated from the ethanol extract of O. fragrans flowers, namely, quercetin, rutin, genistin, kaempferol, isorhamnetin, and naringin, which have different anti-inflammatory, DPPH-free radical scavenging, and anti-proliferation activities. 13 A secoiridoid glycoside (8E-ligstroside) and a coumaric acid analog with a monoterpene moiety (floraosmanol A) inhibit the activity of beta-secretase and nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 macrophages, respectively.…”