HPCPC Separation of Proteins using Polyethylene Glycol-Potassium Phosphate Aqueous Two-Phase

Chen, J.; X., G.; Li, D. Q.

doi:10.1080/10826069908544935

Cited by 24 publications

(14 citation statements)

References 9 publications

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“…3,6,7 Because of the inherent aqueous environment, ABSs have great potential for the separation and purification of a number of biomolecules such as cells, proteins, and nucleic acids, through their partitioning between two aqueous liquid phases. 1,3,4 Recent studies in ABSs have suggested ILs, a new class of electrolytes that, unlike the common salts are liquid below 373.15 K, could be good candidates for extraction and separation purposes. 8−12 The special ambient-friendly physicochemical properties of ILs, such as negligible vapor pressure, nonflammability under ambient conditions, large liquid temperature range, high thermal and chemical stability, strong solubility power, and a number of possible variations in cation and anion features that allow the fine-tuning of their properties, make them suitable candidates as alternative replacements for the volatile and hazardous organic solvents presently used in a wide range of industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Capability of Ionic Liquid–Amino Acid Aqueous Systems for the Formation of Aqueous Biphasic Systems and Their Applications in Extraction

Noshadi

Sadeghi

2017

J. Phys. Chem. B

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To obtain further experimental evidence for a better understanding of the molecular mechanisms responsible for the soluting-out effect phenomena in ternary systems composed of an ionic liquid (IL), amino acid, and water, systematic studies on the vapor-liquid, liquid-liquid, and solid-liquid equilibrium behavior of aqueous solutions of several ILs were carried out in the presence of a range of amino acids. Water activities for binary and ternary aqueous solutions containing 1-butyl-3-methylimidazolium chloride, [Cmim]Cl, 1-hexyl-3-methylimidazolium chloride, [Cmim]Cl, 1-butyl-3-methyl-imidazolium trifluoromethanesulfonate, [Cmim][CFSO], l-Ser, Gly, Ala, and l-Pro were measured using both vapor pressure osmometry and isopiestic methods. All of the ternary IL + amino acid + water systems show negative deviations from semi-ideal behavior and therefore soluting-out effects have been observed in these systems. In the case of the [Cmim][CFSO] + amino acids aqueous systems, the IL is soluted-out by the amino acids and the soluting-out effect appears by aqueous biphasic system formation. For these systems, the phase diagram and partition coefficient of caffeine were measured at 298.15 K. However, for the [Cmim]Cl and [Cmim]Cl containing systems, the amino acids are soluted-out by the ILs and the soluting-out effect appears by precipitation of the amino acids from the solution, and the solubilities of the amino acids in the aqueous solutions decrease in the presence of [Cmim]Cl and [Cmim]Cl.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Capability of Ionic Liquid–Amino Acid Aqueous Systems for the Formation of Aqueous Biphasic Systems and Their Applications in Extraction

Noshadi

Sadeghi

2017

J. Phys. Chem. B

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“…Separation of biological molecules and particles in polymer‐based ABS was initiated more than half a century by Albertsson,1 and later proved to be of immense utility in chemical as well as in biochemical and cell biological research 1, 3–11. Poly(alkylene glycol)s such as polyethylene glycols (PEGs) are widely used polymers in aqueous biphasic partitioning studies both in basic and applied research, due in part to their biodegradability, low toxicity, low cost, relatively low melting points, and very low volatility 12–14.…”

Section: Introductionmentioning

confidence: 99%

A new aqueous biphasic system containing polypropylene glycol and a water‐miscible ionic liquid

Zafarani-Moattar

Hamzehzadeh

Nasiri

2011

Biotechnology Progress

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In this work, we proposed a novel aqueous biphasic system (ABS) composed of polypropylene glycol P400 (PPG P400) and hydrophilic ionic liquids (IL), 1-alkyl-3-methylimidazolium bromide (alkyl = ethyl or butyl), forming an upper polymer-rich phase and a lower IL-rich phase at ambient temperature. This new ABS can present interesting characteristics shared by ILs and polymers such as low volatility, good solvation ability, tunable physical properties, and high design capacity for achieving task-specific phase components to enhance the partitioning of target species. Ternary phase diagram of the novel ABS formed by PPG 400 and [C(2) mim]Br in water was measured at T = 298.15 K. Factors affecting the binodal curves such as the cation side alkyl chain length and the temperature were also evaluated. The results were successfully interpreted in terms of the kosmotropic/chaotropic nature of ILs. Furthermore, the phase behavior of the PPG-[C(2) mim]Br ABS is described by the NRTL model. Finally, the extraction potential of the proposed ABS was evaluated through its application to the extraction of the essential amino acids such as L-tryptophan and L-tyrosine. The partition coefficients here obtained demonstrated the fine potential of the proposed ABS for biomolecules separation.

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“…Du et al [209] have developed a system for lowspeed rotary countercurrent chromatography by utilizing a convoluted multilayer helical tubing to be used for industrial separation. High-performance centrifugal partition chromatography (HPCPC) is also a practical and suitable method for the separation of biomolecules such as proteins, enzymes, and so forth, particularly on the preparative scale [210].…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

Separation Strategies for Processing of Dilute Liquid Streams

Mandal

Kulkarni

2011

International Journal of Chemical Engineering

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Processing of dilute liquid streams in the industries like food, agro-, biotechnology, pharmaceuticals, environment, and so forth needs special strategy for the separation and purification of the desired product and for environment friendly disposal of the waste stream. The separation strategy adopted to achieve the goal is extremely important from economic as well as from environmental point of view. In the present paper we have reviewed the various aspects of some selected universal separation strategies such as adsorption, membrane separation, electrophoresis, chromatographic separation, and electroosmosis that are exercised for processing of dilute liquid streams.

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HPCPC Separation of Proteins using Polyethylene Glycol-Potassium Phosphate Aqueous Two-Phase

Cited by 24 publications

References 9 publications

Evaluation of the Capability of Ionic Liquid–Amino Acid Aqueous Systems for the Formation of Aqueous Biphasic Systems and Their Applications in Extraction

Evaluation of the Capability of Ionic Liquid–Amino Acid Aqueous Systems for the Formation of Aqueous Biphasic Systems and Their Applications in Extraction

A new aqueous biphasic system containing polypropylene glycol and a water‐miscible ionic liquid

Separation Strategies for Processing of Dilute Liquid Streams

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