Hoxb8 Is Required for Normal Grooming Behavior in Mice

Greer, Joy M.; Capecchi, Mario R.

doi:10.1016/s0896-6273(01)00564-5

Cited by 344 publications

(234 citation statements)

References 42 publications

(5 reference statements)

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“…Although automation in and of itself is not a panacea for neurobehavioural experiments 2 , it allows for addressing an entirely new set of questions about mouse behaviour and to conduct experiments on time scales that are orders of magnitude larger than those traditionally assayed. For example, reported tests of grooming behaviour span time scales of minutes 3,4 , whereas an automated analysis will allow for analysis of this behaviour over hours or even days and weeks.…”

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confidence: 99%

Automated home-cage behavioural phenotyping of mice

et al. 2010

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Neurobehavioural analysis of mouse phenotypes requires the monitoring of mouse behaviour over long periods of time. In this study, we describe a trainable computer vision system enabling the automated analysis of complex mouse behaviours. We provide software and an extensive manually annotated video database used for training and testing the system. Our system performs on par with human scoring, as measured from ground-truth manual annotations of thousands of clips of freely behaving mice. As a validation of the system, we characterized the home-cage behaviours of two standard inbred and two non-standard mouse strains. From these data, we were able to predict in a blind test the strain identity of individual animals with high accuracy. Our video-based software will complement existing sensor-based automated approaches and enable an adaptable, comprehensive, high-throughput, fi ne-grained, automated analysis of mouse behaviour. A utomated quantitative analysis of mouse behaviour will have a signifi cant role in comprehensive phenotypic analyses -both on the small scale of detailed characterization of individual gene mutants and on the large scale of assigning gene function across the entire mouse genome 1 . One key benefi t of automating behavioural analysis arises from inherent limitations of human assessment, namely, cost, time and reproducibility. Although automation in and of itself is not a panacea for neurobehavioural experiments 2 , it allows for addressing an entirely new set of questions about mouse behaviour and to conduct experiments on time scales that are orders of magnitude larger than those traditionally assayed. For example, reported tests of grooming behaviour span time scales of minutes 3,4 , whereas an automated analysis will allow for analysis of this behaviour over hours or even days and weeks.Indeed, the signifi cance of alterations in home-cage behaviour has recently gained attention as an eff ective means of detecting perturbations in neural circuit function -both in the context of disease detection and more generally to measure food consumption and activity parameters 5 -10 . Previous automated systems (see refs 8, 9, 11, 12 and Supplementary Note ) rely mostly on the use of simple detectors such as infrared beams to monitor behaviour. Th ese sensor-based approaches tend to be limited in the complexity of the behaviour that they can measure, even in the case of costly commercial systems using transponder technologies 13 . Although such systems can be used eff ectively to monitor locomotor activity and perform operant conditioning, they cannot be used to study homecage behaviours such as grooming, hanging, jumping and smaller movements (termed ' micromovements ' below). Visual analysis is a potentially powerful complement to these sensor-based approaches for the recognition of such fi ne animal behaviours.Advances in computer vision and machine learning over the last decade have yielded robust computer vision systems for the recognition of objects 14,15 and human actions (see Moeslund et ...

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confidence: 99%

Automated home-cage behavioural phenotyping of mice

et al. 2010

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“…In animals, there are also miRNAs which directly degrade their targeted mRNAs. For example, miR-196 directly cleaves the mRNA of HOXB8 (Mansfield et al, 2004;Yekta et al, 2004;Hornstein et al, 2005), which play important role in animal development (van den Akker et al, 1999;Greer and Capecchi, 2002;Reilly, 2002;El-Mounayri et al, 2005).…”

Section: Microrna Biogenesismentioning

confidence: 99%

MicroRNAs and their regulatory roles in animals and plants

Zhang

Wang

Pan

2006

Journal Cellular Physiology

488

307

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microRNAs (miRNAs) are an abundant class of newly identified endogenous non-protein-coding small RNAs. They exist in animals, plants, and viruses, and play an important role in gene silencing. Translational repression, mRNA cleavage, and mRNA decay initiated by miRNA-directed deadenylation of targeted mRNAs are three mechanisms of miRNA-guided gene regulation at the posttranscriptional levels. Many miRNAs are highly conserved in animals and plants, suggesting that they play an essential function in plants and animals. Lots of investigations indicate that miRNAs are involved in multiple biological processes, including stem cell differentiation, organ development, phase change, signaling, disease, cancer, and response to biotic and abiotic environmental stresses. This review provides a general background and current advance on the discovery, history, biogenesis, genomics, mechanisms, and functions of miRNAs.

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“…Compulsive grooming leading to hair removal and self-inflicted wounds has been identified as a major behavioral phenotype of the Hoxb8 homozygous mutant mouse [21]. These mice spent almost twice the time self-grooming as wild-type mice and excessively groomed or barbered control cagemates.…”

Section: Cns Insult and Repetitive Behavior In Animals-mentioning

confidence: 99%

Animal models of restricted repetitive behavior in autism

Lewis

Tanimura

Lee

et al. 2007

Behavioural Brain Research

229

227

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Restricted, repetitive behavior, along with deficits in social reciprocity and communication, is diagnostic of autism. Animal models relevant to this domain generally fall into three classes: repetitive behavior associated with targeted insults to the CNS; repetitive behavior induced by pharmacological agents; and repetitive behavior associated with restricted environments and experience. The extant literature provides potential models of the repetitive behavioral phenotype in autism rather than attempts to model the etiology or pathophysiology of restricted, repetitive behavior, as these are poorly understood. This review focuses on our work with deer mice which exhibit repetitive behaviors associated with environmental restriction. Repetitive behaviors are the most common category of abnormal behavior observed in confined animals and larger, more complex environments substantially reduce the development and expression of such behavior. Studies with this model, including environmental enrichment effects, suggest alterations in cortical-basal ganglia circuitry in the development and expression of repetitive behavior. Considerably more work needs to be done in this area, particularly in modeling the development of aberrant repetitive behavior. As mutant mouse models continue to proliferate, there should be a number of promising genetic models to pursue.

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Hoxb8 Is Required for Normal Grooming Behavior in Mice

Cited by 344 publications

References 42 publications

Automated home-cage behavioural phenotyping of mice

Automated home-cage behavioural phenotyping of mice

MicroRNAs and their regulatory roles in animals and plants

Animal models of restricted repetitive behavior in autism

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