How to quantify iron in an aqueous or biological matrix: a technical note

Boutry, S; Forge, Delphine; Burtéa, Carmen; Mahieu, Isabelle; Murariu, Oltea; Laurent, Sophie; Elst, Luce Vander; Müller, Robert N.

doi:10.1002/cmmi.291

Cited by 78 publications

(100 citation statements)

References 12 publications

(10 reference statements)

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“…SPION iron concentrations were quantified by using the Prussian Blue colorimetric assay. 60 Nanoparticles were dissolved in a 6 M HCl/0.01 M HNO 3 solution. Then, 5% w/v of freshly prepared potassium hexacyanoferrate(II) trihydrate was added, and the absorbance at 690 nm was read after 10 min on a VICTOR3 1420 Multilabel Counter (PerkinElmer).…”

Section: Experimental Methodsmentioning

confidence: 99%

Lock-In Thermography as an Analytical Tool for Magnetic Nanoparticles: Measuring Heating Power and Magnetic Fields

et al. 2017

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Magnetic nanoparticles and their ability to convert electromagnetic energy into heat are of explicit interest for various applications. However, precise quantification of their heating efficiency is not always upfront, and several parameters render comparative studies challenging. This paper describes the theory behind lock-in thermography, a new technique for quantifying the heating properties of magnetic nanoparticles. This technique allows the investigation of some of the potential sources of variability: key factors such as magnetic field inhomogeneity and its effects on the heating power are explored in detail. The presented results, obtained from various nanoparticle batches of different origins, highlight the importance of pursuing a standardized and systematic approach when quantifying the heating efficiency of magnetic nanoparticles.

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Section: Experimental Methodsmentioning

confidence: 99%

Lock-In Thermography as an Analytical Tool for Magnetic Nanoparticles: Measuring Heating Power and Magnetic Fields

et al. 2017

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“…This method is based on an intrinsic property of iron oxide nanoparticles (IONPs) to increase the water proton relaxation rates (R 1 or R 2 ) by a value that is correlated to its concentration in the solution, cell suspension or tissue [25]. To quantify the iron from cellular suspension, 10 6 cells were mineralized in 100 µL 5 N HCl on a water bath at 80 • C for 4 h. In order to determine the longitudinal relaxation rate R 1 of iron from the digested cell samples, T 1 (longitudinal) relaxation time was measured at 37 • C and 1.4 T (60 MHz) on a Bruker Minispec mq60 (Karlsruhe, Germany).…”

Section: Relaxometry Techniquementioning

confidence: 99%

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Balaș

Ciobanu

Burtéa

et al. 2017

Metals

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Abstract:We report the synthesis of dextran-coated iron oxide magnetic nanoparticles (DIO-NPs) with spherical shape and uniform size distribution as well as their accumulation and toxic effects on Jurkat cells up to 72 h. The characterization of dextran-coated maghemite nanoparticles was done by X-ray diffraction and dynamic light scattering analyses, transmission electron microscopy imaging, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, magnetic hysteresis, and relaxometry measurements. The quantification of DIO-NPs intracellular uptake showed a progressive accumulation of iron as a function of time and dose accompanied by additional lysosome formation and an increasing darkening exhibited by a magnetic resonance imaging (MRI) scanner. The cytotoxicity assays revealed a decrease of cell viability and a loss of membrane integrity in a time-and dose-dependent manner. Exposure to DIO-NPs determined an increase in reactive oxygen species level up to 72 h. In the first two days of exposure, the level of reduced glutathione decreased and the amount of malondyaldehyde increased, but at the end of the experiment, their concentrations returned to control values. These nanoparticles could be used as contrast agents for MRI but several parameters concerning their interaction with the cells should be taken into consideration for a safe utilization.

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“…Temporal variation in the concentration of the fraction deposited was determined using SPION phantoms dispersed in the culture medium (Figure 2). Relaxometric measures were used according to the method described by Boutry et al 34 …”

Section: Fraction Of Dose Depositedmentioning

confidence: 99%

Particokinetics: computational analysis of the superparamagnetic iron oxide nanoparticles deposition process

Cárdenas¹,

Mamani²,

Sibov³

et al. 2012

IJN

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Background: Nanoparticles in suspension are often utilized for intracellular labeling and evaluation of toxicity in experiments conducted in vitro. The purpose of this study was to undertake a computational modeling analysis of the deposition kinetics of a magnetite nanoparticle agglomerate in cell culture medium. Methods: Finite difference methods and the Crank-Nicolson algorithm were used to solve the equation of mass transport in order to analyze concentration profiles and dose deposition. Theoretical data were confirmed by experimental magnetic resonance imaging. Results: Different behavior in the dose fraction deposited was found for magnetic nanoparticles up to 50 nm in diameter when compared with magnetic nanoparticles of a larger diameter. Small changes in the dispersion factor cause variations of up to 22% in the dose deposited. The experimental data confirmed the theoretical results. Conclusion: These findings are important in planning for nanomaterial absorption, because they provide valuable information for efficient intracellular labeling and control toxicity. This model enables determination of the in vitro transport behavior of specific magnetic nanoparticles, which is also relevant to other models that use cellular components and particle absorption processes.

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How to quantify iron in an aqueous or biological matrix: a technical note

Cited by 78 publications

References 12 publications

Lock-In Thermography as an Analytical Tool for Magnetic Nanoparticles: Measuring Heating Power and Magnetic Fields

Lock-In Thermography as an Analytical Tool for Magnetic Nanoparticles: Measuring Heating Power and Magnetic Fields

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Particokinetics: computational analysis of the superparamagnetic iron oxide nanoparticles deposition process

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