How to Pack the Genome for a Safe Trip

Caron, Cécile; Govin, Jérôme; Rousseaux, Sophie; Khochbin, Saadi

doi:10.1007/3-540-27310-7_3

Cited by 49 publications

(34 citation statements)

References 92 publications

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“…During the spermatogenesis process and sperm maturation in the epididymis and post-ejaculatory capacitation in the female reproductive tract, post-translational modifications (PTM) are critical for the functionality of spermatozoa [208][209][210]. PTMs that are formed by glycosylation, methylation, or phosphorylation are able to change the functional properties of spermatozoa and seminal plasma proteins [211].…”

Section: Power Of Proteomics In Male Infertilitymentioning

confidence: 99%

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Tahmasbpour

Balasubramanian

Agarwal

2014

J Assist Reprod Genet

107

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Background The assisted reproductive techniques aimed to assist infertile couples have their own offspring carry significant risks of passing on molecular defects to next generations. Results Novel breakthroughs in gene and protein interactions have been achieved in the field of male infertility using genome-wide proteomics and transcriptomics technologies. Conclusion Male Infertility is a complex and multifactorial disorder.Significance This review provides a comprehensive, up-todate evaluation of the multifactorial factors involved in male infertility. These factors need to be first assessed and understood before we can successfully treat male infertility.

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Section: Power Of Proteomics In Male Infertilitymentioning

confidence: 99%

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Tahmasbpour

Balasubramanian

Agarwal

2014

J Assist Reprod Genet

107

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“…Indeed, histones are first replaced with transition proteins and then with Sperm Nuclear Basic Proteins (SNBPs) during the condensation phase of spermatid nuclei. SNBPs include testis specific histone variants but also non-histone proteins such as protamine-like proteins and protamines (Caron et al, 2005;Govin et al, 2004;Lewis et al, 2003;Poccia and Collas, 1996). The sperm chromatin structure is highly diverse in animals, even between species of the same animal group (Frehlick et al, 2006).…”

Section: Spermiogenesismentioning

confidence: 99%

Epigenetic and replacement roles of histone variant H3.3 in reproduction and development

Orsi¹,

Couble²,

Loppin³

2009

Int. J. Dev. Biol.

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The nucleosomal organization of eukaryotic chromatin is generally established during DNA replication by the deposition of canonical histones synthesized in S phase. However, cells also use a Replication Independent (RI) nucleosome assembly pathway that allows the incorporation of non-canonical histone variants in the chromatin. H3.3 is a conserved histone variant that is structurally very close to its canonical counterpart but nevertheless possesses specific properties. In this review, we discuss the dual role of H3.3 which functions as a neutral replacement histone, but also participates in the epigenetic transmission of active chromatin states. These properties of H3.3 are also explored in the light of recent studies that implicate this histone and its associated chromatin assembly factors in large scale, replication-independent chromatin remodeling events. In particular, H3.3 appears as a critical player in the transmission of the paternal genome, from sperm to zygote.

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“…During spermiogenesis, histone proteins are replaced sequentially by transition proteins (TPs) and protamines (Brewer et al, 2002). The histone-TP/protamine exchange, incorporation of testis-specific histone variants, and stage-specific histone modifications lead to a highly compact chromatin structure in the sperm (Braun, 1998;Caron et al, 2005). This chromatin structure may repress transcription (SassoneCorsi, 2002) and is therefore postulated to be relevant with regard to transcription silencing.…”

Section: Introductionmentioning

confidence: 99%

Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

Zheng

Xia

Ding

et al. 2008

Developmental Dynamics

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Male germ cells possess a unique epigenetic program and express a male-specific transcription profile. However, when its chromatin is passed onto the zygote, it expresses an transcription/epigenetic program characteristic of the zygote. The mechanism underlying this reprogramming process is not understood at present. In this study, we show that an extensive range of chromatin factors (CFs), including essential transcription factors and regulators, remodeling factors, histone deacetylases, heterochromatin-binding proteins, and topoisomerases, were removed from chromatin during spermiogenesis. This process will erase the paternal epigenetic program to generate a relatively naive chromatin, which is likely to be essential for installation of the zygotic developmental program after fertilization. We have also showed that transcription termination in male germ cells was temporally correlated with CF dissociation. A genome-wide CF dissociation will inevitably disassemble the transcription apparatus and regulatory mechanism and lead to transcription silence. Based on data presented in this and previous studies (Sun et al., Cell Research [2007] 17:117-134), we propose that paternal-zygotic transcription reprogramming begins with a genome-wide CF dissociation to erase the existing transcription program in later stages of spermatogenesis. This will be followed by assembling of the zygotic equivalent after fertilization. The transcription/epigenetic program of the male germ cell is transformed into a zygotic one using an erase-and-rebuild strategy similar to that used in the maternal-zygotic transition. It is also noted that transcription is terminated long after meiosis is completed and before chromatin becomes highly condensed during spermatogenesis. The temporal order of these events suggests that transcription silence does not have to be coupled to meiosis or chromatin condensation.

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How to Pack the Genome for a Safe Trip

Cited by 49 publications

References 92 publications

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Epigenetic and replacement roles of histone variant H3.3 in reproduction and development

Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

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