How to improve the efficiency of a traditional dissolution dynamic nuclear polarization (dDNP) apparatus: Design and performance of a fluid path compatible dDNP/LOD-ESR probe

Lê, Thanh Phong; Hyacinthe, Jean-Noël; Capozzi, Andrea

doi:10.1016/j.jmr.2022.107197

Cited by 7 publications

(43 citation statements)

References 41 publications

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“…The coil is remotely tuned and matched outside of the cryostat by means of a T/M box with two piston trimmer capacitors (Voltronics V1949) connected to the cryogenic probe through the SMA (Fig. 2 B, 5) 28 , 29 . The T/M box can cover the range 38–42 MHz.…”

Section: Methodsmentioning

confidence: 99%

Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

Capozzi

2022

Sci Rep

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As of today, dissolution Dynamic Nuclear Polarization (dDNP) is the only clinically available hyperpolarization technique for 13C-MRI. Despite the clear path towards personalized medicine that dDNP is paving as an alternative and/or complement to Positron Emission Tomography (PET), the technique struggles to enter everyday clinical practice. Because of the minute-long hyperpolarization lifetime after dissolution, one of the reasons lies in the need and consequent complexities of having the machine that generates the hyperpolarization (i.e. the dDNP polarizer) on site. Since some years, research groups are working to make hyperpolarization transportable. Two different methods have been developed that allow “freezing” of the nuclear spin state prior to samples extraction from the polarizer. Nevertheless, so far, all attempts of transport have been limited to a very small scale and to the level of proof-of-principle experiments. The main reason for that is the lack of adequate hardware, strategy, and control on most of the crucial parameters. To bridge the technical gap with PET and provide MRI facilities with hours long relaxing hyperpolarized compounds at controlled conditions, a new generation of low cost/small footprint liquid He cryostats equipped with a magnetically enforced cryogenic probe is needed. In this paper, we detail the theoretical and practical construction of a hyperpolarized samples transportation device small enough to fit in a car and able to hold a sample at 4.2 K for almost 8 h despite the presence of a cryogenically-demanding purpose-built probe that provides enough magnetic field upon insertion of the sample and NMR quality homogeneity at storage position. Should transportable hyperpolarization via DNP become a reality, we herein provide important details to make it possible.

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Section: Methodsmentioning

confidence: 99%

Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

Capozzi

2022

Sci Rep

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“…In the present work, we detail the design and performance of a highly versatile and customizable dDNP cryogenic probe, herein adapted to a 5 T "wet" preclinical polarizer 41 , that, employing a Custom Fluid Path (CFP) 18,42 , can not only co-polarize up to three samples, but it is also capable of monitoring the solid-state dynamic of each of them separately, thanks to dedicated pseudo-Alderman-Grant (AG) coils and multi-nucleus parallel Nuclear Magnetic Resonance (NMR) acquisition on three distinct channels. We tested the system for the generation of three HP solutions of the same substrate (i.e.…”

Section: Was Invented In 2003 Bymentioning

confidence: 99%

“…In Fig. 1, we report 3D drawings of the multi-sample dDNP cryogenic probe adapted to our 5 T "wet" polarizer, whose design, functioning principle, retro t and performance were described in detail earlier 27,41 . All main components are indicated by capital letters.…”

Section: Multi-sample Dnp Probe Design and Nmr Setupmentioning

confidence: 99%

“…Below the ISO-KF40 ange, brazed gold-plated ba es offer mechanical support to the sample tubes, the coaxial cables and the waveguide, and reduce heat transfer via convection and radiation 16,41 . The top of a gold-plated microwave cavity (Q) is brazed to the lower end of the sample tubes.…”

Section: Multi-sample Dnp Probe Design and Nmr Setupmentioning

confidence: 99%

“…In this way, the microwave beam splits in three portions that invest perpendicularly each sample through one opening of the AG coil. Each AG coil is remotely tuned and matched outside of the VTI with a coaxial cable and a tuning/matching network mounting two 1.5-250 pF piston trimmer capacitors (Knowles-Voltronics V1949, Itasca, USA) 27,41 . The inside of the microwave cavity makes a tight t around the coil formers.…”

Section: Multi-sample Dnp Probe Design and Nmr Setupmentioning

confidence: 99%

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Highly versatile dissolution DNP: implementation of a multi- sample/multi-nucleus cryogenic probe with parallel NMR detection

Lê

Hyacinthe

Capozzi

2023

Preprint

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Dissolution Dynamic Nuclear Polarization (dDNP) is the most versatile hyperpolarization technique to enhance NMR sensitivity in the liquid state. The unprecedented signal enhancement is the key for a large range of applications spanning from fast chemical reaction monitoring to metabolism investigation in real time. Unfortunately, this exceptional time resolution does not come without a price. Low throughput is one of dDNP main shortcomings. Especially for clinical and preclinical applications, where direct 13C nuclei polarization is usually pursued, it takes hours to generate one single hyperpolarized (HP) sample. Therefore, being able to hyperpolarize more samples at once represents a clear advantage and can expand the range and complexity of the applications. Some clinical and preclinical systems are equipped with a multi-sample option. Nevertheless, the solid-state NMR detection is far from optimal, not being sample selective or requiring the displacement of the sample to acquire and monitor its signal. In this work, we present the design and performance of a highly versatile and customizable dDNP cryogenic probe, herein adapted to a 5 T “wet” preclinical polarizer, that can not only accommodate up to three Custom Fluid Paths (CFPs), but it is also capable of monitoring the solid-state dynamics of each sample separately, thanks to dedicated pseudo-Alderman-Grant coils and multi-nuclei parallel Nuclear Magnetic Resonance (NMR) acquisition on three distinct channels. Within 30 min, the system was able to dispense three HP solutions with high repeatability across the channels (30.0 ± 1.2% carbon polarization for [1-13C]pyruvic acid doped with trityl radical). Moreover, we tested multi-nucleus NMR capability by polarizing and monitoring simultaneously 13C, 1H and 129Xe. Finally, we implemented [1-13C]lactate/[1-13C]pyruvate polarization and back-to-back dissolution and injection in a healthy mouse model to perform multiple-substrate HP Magnetic Resonance Spectroscopy (MRS) at 14.1 T.

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Hyperpolarized tracer design, synthesis, and characterization

Suh,

Eskandari,

Huynh

et al. 2024

Advances in Magnetic Resonance Technology and Applications

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How to improve the efficiency of a traditional dissolution dynamic nuclear polarization (dDNP) apparatus: Design and performance of a fluid path compatible dDNP/LOD-ESR probe

Cited by 7 publications

References 41 publications

Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

Highly versatile dissolution DNP: implementation of a multi- sample/multi-nucleus cryogenic probe with parallel NMR detection

Hyperpolarized tracer design, synthesis, and characterization

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