How to improve IVF–ICSI outcome by sperm selection

Abstract

In previous studies, a new IVF method of intracytoplasmic morphologically selected sperm injection (IMSI) was introduced, based on motile sperm organellar morphology examination (MSOME). It was concluded that microinjection of morphologically selected sperm cells with strictly normal nucleus, defined by MSOME, improves IVF-ICSI outcome. The aim of the present study was to confirm this conclusion in new, enlarged study groups. Comparison between 80 couples, who underwent an IVF-IMSI trial, with matched couples, who underwent a standard IVF-ICSI procedure, confirmed that pregnancy rate following IVF-IMSI was significantly higher, and abortion rate significantly lower than in the routine IVF-ICSI (60.0 versus 25.0%, and 14 versus 40% respectively, P <or= 0.05). Another comparison was performed between matched IMSI groups, where embryos were obtained from microinjection by spermatozoa with a morphologically normal nucleus ('best' group, n = 70) and a 'second best' group was selected, where embryos were obtained from microinjection of spermatozoa with minimal morphological impairment, since no other sperm cells were available. It was confirmed that microinjection by 'second best ' spermatozoa result in significantly lower pregnancy and delivery rates and significantly higher abortion rates than microinjection with 'best' spermatozoa (25.7 versus 58.2%, P <or= 0.01; 17.1 versus 52.8%, P <or= 0.01, and 33.3 versus 9.7%, P <or= 0.02 respectively). The present study has strengthened previous conclusions.

Male age influences oocyte-donor program results

“…This point implies the need for the employment of a higher optical magnification for sperm selection, especially for ICSI. Such a new proposal is important and could be implemented not only in couples with previous ICSI failures, as performed by Bartoov's methodology [26], but in general use for each routine ICSI procedure.…”

“…Young women, of an average age of 24 (range [20][21][22][23][24][25][26], were accepted into the Egg Donation Program (EDP) cycles. All of these women were healthy, had normal menstrual cycles and no determined pathology.…”

Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

“…During ART procedures, semen is traditionally processed with the density gradient centrifugation or swim‐up techniques 5, 6. With ICSI, individual sperm are then selected visually based on motility and morphology by a trained embryologist for injection to an egg 7, 8. However, other important sperm attributes that can affect ART outcomes, including sperm DNA–chromatin or chromosomal integrity, mutational, or methylome composition, and the presence of reactive oxygen species (ROS)9, 10, 11, 12, 13, 14, 15, 16, 17 are typically not considered.…”

The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging—a retrospective observational study

“…It has been reported that the negative impact of large nuclear vacuoles (LNV) is perceptible after the onset of the EGA, leading to reduced blastocyst formation [28][29][30][31]. Moreover, LNV also affect ongoing pregnancy rates [31][32][33][34], miscarriage rates [31,33,[35][36][37], and malformation in offspring [38,39].…”