Background: Several chemical pollutants can accumulate in closed spaces of transportation and storage of non-dangerous goods, leading to high concentrations. Pollutants are mainly residues of pesticides used to prevent the spread of harmful organisms in intercontinental transportation, volatile organic compounds and components of diesel engine exhaust. The objectives of this cross-sectional qualitative study were to identify and evaluate the regulations in connection with the occupational chemical exposures caused by chemical pollutants in closed environments of transportation and storage, and to survey the knowledge, attitudes and practices (KAP) of occupational health and safety professionals and logistics managers related to this global workplace issue. Methods: A comprehensive systematic search of legal instruments was carried out in international, European Union and Hungarian legislation databases. Legal documents relevant for occupational chemical exposure at workplaces were included in the study and the legal relationships between selected documents were mapped. The systematic search of legal instruments identified 4737 records, of which 16 were included in the in-depth content analysis. According to the second objective, semi-structured face-to-face interviews were carried out with occupational health and safety professionals and warehouse managers at logistics companies located in Hungary. Results: The analysis of the documents highlighted the lack of explicit regulation on prevention from exposures caused by chemical pollutants in closed environments of transportation and storage of non-hazardous materials. The 21 completed interviews revealed that the professionals had very limited knowledge about the potential presence of chemical residues in closed spaces of transportation and storage. They deemed such chemical exposure rare and the related health effects negligible. Although legislation requires the risk assessment of workplaces, the assessment is misleading if potential hazards are not identified. Conclusions: The increasing risk of rapid global spread of harmful organisms due to climate change and dense international traffic results in growing need for fumigation, which, together with increasing temperature and transportation time, generates an emerging occupational and public health challenge. The revealed limitations point out that the chemical safety of transportation and storage of non-dangerous goods should be more specifically regulated and responsible professionals should be better informed about such workplace hazards.