How to Deal with workforce stress

Morris, Glyn

doi:10.1108/shr.2010.37209cab.001

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While differences in the onset of O-glycosylation between males and females as observed in our study are striking, our exhaustive literature search did not reveal any studies in connection with hormonal regulation of O-glycosylation effects that would justify the reason behind this observation. Besides the well-known studies on pregnancy-associated changes in glycodelin 47 and uromodulin 48 conducted in the laboratories of Howard Morris and Ann Dell two decades ago, the literature on hormonally-dependent N-glycosylation is not particularly abundant either. The relatively recent reports include post-translationallyinduced functional targeting of the hormone thyrotropin 49 , N-glycome changes in plasma during pregnancy 50 , and the sex-related differences in N-glycosylation of IgG in both humans and mice [51][52][53] .…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients

2019

View full text Add to dashboard Cite

A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients. The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile. This is far more detected components than shown in any previous studies. The analytical results were further subjected to a battery of statistical tests. Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples. While the control vs. cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs. female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation. Our results thus underscore the necessity of applying new analytical technologies to clinically interesting sample sets.

show abstract