How to B(e)-1 Important Cell During Leishmania Infection

Firmino-Cruz, Luan; Decotè-Ricardo, Débora; Gomes, Daniel; Morrot, Alexandre; Freire-de-Lima, Célio Geraldo; Guedes, Herbert Leonel de Matos

doi:10.3389/fcimb.2019.00424

Cited by 11 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Impaired humoral immunity could play a critical role in the progression of CanL, as hypergammaglobulinemia, nonspecific polyclonal antibodies and circulating immune complexes correlate with clinical progression of the disease (12,(141)(142)(143). However, participation of B cells in the immunopathogenesis of leishmaniosis is not only related to antibody production, but also to their functions as regulatory and APCs (144).…”

Section: B Cell Dysfunction and Humoral Immunitymentioning

confidence: 99%

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

et al. 2022

View full text Add to dashboard Cite

Leishmania infantum, the etiological agent of canine leishmaniosis (CanL) in Europe, was responsible of the largest outbreak of human leishmaniosis in Spain. The parasite infects and survives within myeloid lineage cells, causing a potentially fatal disease if left untreated. The only treatment option relies on chemotherapy, although immunotherapy strategies are being considered as novel approaches to prevent progression of the disease. To this aim, a deeper characterization of the molecular mechanisms behind the immunopathogenesis of leishmaniosis is necessary. Thus, we evaluated, for the first time, the host immune response during L. infantum infection through transcriptome sequencing of the popliteal lymph nodes aspirates of dogs with CanL. Differential expression and weighted gene co-expression network analyses were performed, resulting in the identification of 5,461 differentially expressed genes (DEGs) and four key modules in sick dogs, compared to controls. As expected, defense response was the highest enriched biological process in the DEGs, with six genes related to immune response against pathogens (CHI3L1, SLPI, ACOD1, CCL5, MPO, BPI) included among the ten most expressed genes; and two of the key co-expression modules were associated with regulation of immune response, which also positively correlated with clinical stage and blood monocyte concentration. In particular, sick dogs displayed significant changes in the expression of Th1, Th2, Th17 and Tr1 cytokines (e. g. TNF-α, IFN-γ, IL-21, IL-17, IL-15), markers of T cell and NK cell exhaustion (e. g. LAG3, CD244, Blimp-1, JUN), and B cell, monocyte and macrophage disrupted functionality (e. g. CD40LG, MAPK4, IL-1R, NLRP3, BCMA). In addition, we found an overexpression of XBP1 and some other genes involved in endoplasmic reticulum stress and the IRE1 branch of the unfolded protein response, as well as one co-expression module associated with these processes, which could be induced by L. infantum to prevent host cell apoptosis and modulate inflammation-induced lymphangiogenesis at lymph nodes. Moreover, 21 lncRNAs were differentially expressed in sick dogs, and one key co-expression module was associated with chromatin organization, suggesting that epigenetic mechanisms could also contribute to dampening host immune response during natural L. infantum infection in the lymph nodes of dogs suffering from clinical leishmaniosis.

show abstract

Section: B Cell Dysfunction and Humoral Immunitymentioning

confidence: 99%

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

et al. 2022

View full text Add to dashboard Cite

show abstract

“…However, it has been shown in Paracoccidioides brasiliensis infection that B-1 cells secretes IL-10 and induces T regulatory cells activation facilitating the progression of the disease 26 , 27 . This contribution of IL-10 production by B-1 cells was also seen in Leishmania and Francisella infections 28 – 30 . Therefore, B-1 cells appear to differently commit their effector function in distinct infections.…”

Section: Introductionmentioning

confidence: 53%

“…Studies using B-1 cells of deficient mice (XID mice), suggested an important role for these cells during the immune responses against infections 29 , 30 , 34 , 35 . This led us to investigate if the B-1 cells would be implyed in the response against C. gattii infection on this animal model.…”

Section: Resultsmentioning

confidence: 99%

“…The role of B-1 lymphocytes in antibody production has been characterized. However, these cells also have other characteristics that can play important mechanisms in protecting against different pathogens, and one of these mechanisms is their ability to differentiate into phagocytes called B-1 cell-derived phagocytes (B-1CDP) 30 , 34 , 72 – 74 . It was also shown that B-1 cells migrate to the inflammatory/infectious focus and differentiate into macrophage-like cells 75 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

X-linked immunodeficient (XID) mice exhibit high susceptibility to Cryptococcus gattii infection

Diniz-Lima

Rosa

Silva-Junior

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Cryptococcosis is an opportunistic disease caused by the fungus Cryptococcus neoformans and Cryptococcus gattii. It starts as a pulmonary infection that can spread to other organs, such as the brain, leading to the most serious occurrence of the disease, meningoencephalitis. The humoral response has already been described in limiting the progression of cryptococcosis where the B-1 cell seems to be responsible for producing natural IgM antibodies, crucial for combating fungal infections. The role of the B-1 cell in C. neoformans infection has been initially described, however the role of the humoral response of B-1 cells has not yet been evaluated during C. gattii infections. In the present study we tried to unravel this issue using XID mice, a murine model deficient in the Btk protein which compromises the development of B-1 lymphocytes. We use the XID mice compared to BALB/c mice that are sufficient for the B-1 population during C. gattii infection. Our model of chronic lung infection revealed that XID mice, unlike the sufficient group of B-1, had early mortality with significant weight loss, in addition to reduced levels of IgM and IgG specific to GXM isolated from the capsule of C. neoformans. In addition to this, we observed an increased fungal load in the blood and in the brain. We described an increase in the capsular size of C. gattii and the predominant presence of cytokines with a Th2 profile was also observed in these animals. Thus, the present study strongly points to a higher susceptibility of the XID mouse to C. gattii, which suggests that the presence of B-1 cells and anti-GXM antibodies is fundamental during the control of infection by C. gattii.

show abstract

“…Several studies confirmed that murine B1-CDP cells could phagocytose solid particles, such as latex beads and microbes, in vivo. Interestingly, B1-CDP cells had a significantly higher phagocytic activity than peritoneal macrophages [94][95][96][124][125][126][127].…”

Section: Phagocytosis-eating For Eliminationmentioning

confidence: 99%

The Forgotten Brother: The Innate-like B1 Cell in Multiple Sclerosis

et al. 2022

View full text Add to dashboard Cite

Multiple sclerosis (MS) is a neurodegenerative disease of the central nervous system (CNS), traditionally considered a chronic autoimmune attack against the insulating myelin sheaths around axons. However, the exact etiology has not been identified and is likely multi-factorial. Recently, evidence has been accumulating that implies that autoimmune processes underlying MS may, in fact, be triggered by pathological processes initiated within the CNS. This review focuses on a relatively unexplored immune cell—the “innate-like” B1 lymphocyte. The B1 cell is a primary-natural-antibody- and anti-inflammatory-cytokine-producing cell present in the healthy brain. It has been recently shown that its frequency and function may differ between MS patients and healthy controls, but its exact involvement in the MS pathogenic process remains obscure. In this review, we propose that this enigmatic cell may play a more prominent role in MS pathology than ever imagined. We aim to shed light on the human B1 cell in health and disease, and how dysregulation in its delicate homeostatic role could impact MS. Furthermore, novel therapeutic avenues to restore B1 cells’ beneficial functions will be proposed.

show abstract

How to B(e)-1 Important Cell During Leishmania Infection

Cited by 11 publications

References 44 publications

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

X-linked immunodeficient (XID) mice exhibit high susceptibility to Cryptococcus gattii infection

The Forgotten Brother: The Innate-like B1 Cell in Multiple Sclerosis

Contact Info

Product

Resources

About