How the mechanical microenvironment of stem cell growth affects their differentiation: a review

Zhang, Xiaofang; Zhang, Sibo; Wang, Tian-Lu

doi:10.1186/s13287-022-03070-0

Cited by 27 publications

(18 citation statements)

References 174 publications

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“…In addition, MSCs and fibroblasts have been identified as appropriate mechanical stimulatory parameters for regulating proliferation [ 28 ]. In the aspect of differentiation potential, accumulating evidence have shown that the mechanical microenvironments including stiffness and tension influence the osteogenic- and adipogenic-differentiation of ASCs [ 47 , 48 , 49 ]. In addition to the level of mechanical stress, rate of stress relaxation is also identified as the regulator of differentiation of MSCs [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

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Cell-assisted lipotransfer (CAL), defined as co-transplantation of aspirated fat with enrichment of adipose-derived stem cells (ASCs), is a novel technique for cosmetic and reconstructive surgery to overcome the low survival rate of traditional fat grafting. However, clinically approved techniques for increasing the potency of ASCs in CAL have not been developed yet. As a more clinically applicable method, we used mechanical stress to reinforce the potency of ASCs. Mechanical stress was applied to the inguinal fat pad by needling . Morphological and cellular changes in adipose tissues were examined by flow cytometric analysis 1, 3, 5, and 7 days after the procedure. The proliferation and adipogenesis potencies of ASCs were evaluated. CAL with ASCs treated with mechanical stress or sham control were performed, and engraftment was determined at 4 weeks post-operation. Flow cytometry analysis revealed that mechanical stress significantly increased the number as well as the frequency of ASC proliferation in fat. Proliferation assays and adipocyte-specific marker gene analysis revealed that mechanical stress promoted proliferation potential but did not affect the differentiation capacity of ASCs. Moreover, CAL with cells derived from mechanical stress-treated fat increased the engraftment. Our results indicate that mechanical stress may be a simple method for improving the efficacy of CAL by enhancing the proliferation potency of ASCs.

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Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

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“…Considering that the COMPcc coiled-coil’s binding affinity is tunable ( Dooling and Tirrell, 2016 ; Rapp et al, 2017 ), the mechanical properties of both PEP constructs could be altered without changing the chemical properties of the culture medium. Considering recent advances connecting stem cell differentiation to changes in mechanical properties ( Han et al, 2020 ; Hayward et al, 2021 ; Zhang et al, 2022 ), this may be a fruitful area of further research.…”

Section: Discussionmentioning

confidence: 99%

A matrigel-free method for culture of pancreatic endocrine-like cells in defined protein-based hydrogels

Kozlowski

Zook²,

Chigumba³

et al. 2023

Front. Bioeng. Biotechnol.

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The transplantation of pancreatic endocrine islet cells from cadaveric donors is a promising treatment for type 1 diabetes (T1D), which is a chronic autoimmune disease that affects approximately nine million people worldwide. However, the demand for donor islets outstrips supply. This problem could be solved by differentiating stem and progenitor cells to islet cells. However, many current culture methods used to coax stem and progenitor cells to differentiate into pancreatic endocrine islet cells require Matrigel, a matrix composed of many extracellular matrix (ECM) proteins secreted from a mouse sarcoma cell line. The undefined nature of Matrigel makes it difficult to determine which factors drive stem and progenitor cell differentiation and maturation. Additionally, it is difficult to control the mechanical properties of Matrigel without altering its chemical composition. To address these shortcomings of Matrigel, we engineered defined recombinant proteins roughly 41 kDa in size, which contain cell-binding ECM peptides derived from fibronectin (ELYAVTGRGDSPASSAPIA) or laminin alpha 3 (PPFLMLLKGSTR). The engineered proteins form hydrogels through association of terminal leucine zipper domains derived from rat cartilage oligomeric matrix protein. The zipper domains flank elastin-like polypeptides whose lower critical solution temperature (LCST) behavior enables protein purification through thermal cycling. Rheological measurements show that a 2% w/v gel of the engineered proteins display material behavior comparable to a Matrigel/methylcellulose-based culture system previously reported by our group to support the growth of pancreatic ductal progenitor cells. We tested whether our protein hydrogels in 3D culture could derive endocrine and endocrine progenitor cells from dissociated pancreatic cells of young (1-week-old) mice. We found that both protein hydrogels favored growth of endocrine and endocrine progenitor cells, in contrast to Matrigel-based culture. Because the protein hydrogels described here can be further tuned with respect to mechanical and chemical properties, they provide new tools for mechanistic study of endocrine cell differentiation and maturation.

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“…The mechanical microenvironment of stem cells has a huge role in its differentiation, which consists of various factors, including the extracellular matrix and topology, substrate stiffness, shear stress, hydrostatic pressure, tension and microgravity. Nuclear factor-kB, the nicotinic acetylcholine receptor, the piezoelectric mechanosensitive ion channel and HIF-1α are involved in the mechanical modulation process [ 169 ].…”

Section: Application Of O 2 Hypoxia Pathway In Op...mentioning

confidence: 99%

Hypoxia Pathway in Osteoporosis: Laboratory Data for Clinical Prospects

Wang

Zhao

Che

et al. 2023

IJERPH

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The hypoxia pathway not only regulates the organism to adapt to the special environment, such as short-term hypoxia in the plateau under normal physiological conditions, but also plays an important role in the occurrence and development of various diseases such as cancer, cardiovascular diseases, osteoporosis. Bone, as a special organ of the body, is in a relatively low oxygen environment, in which the expression of hypoxia-inducible factor (HIF)-related molecules maintains the necessary conditions for bone development. Osteoporosis disease with iron overload endangers individuals, families and society, and bone homeostasis disorder is linked to some extent with hypoxia pathway abnormality, so it is urgent to clarify the hypoxia pathway in osteoporosis to guide clinical medication efficiently. Based on this background, using the keywords “hypoxia/HIF, osteoporosis, osteoblasts, osteoclasts, osteocytes, iron/iron metabolism”, a matching search was carried out through the Pubmed and Web Of Science databases, then the papers related to this review were screened, summarized and sorted. This review summarizes the relationship and regulation between the hypoxia pathway and osteoporosis (also including osteoblasts, osteoclasts, osteocytes) by arranging the references on the latest research progress, introduces briefly the application of hyperbaric oxygen therapy in osteoporosis symptoms (mechanical stimulation induces skeletal response to hypoxic signal activation), hypoxic-related drugs used in iron accumulation/osteoporosis model study, and also puts forward the prospects of future research.

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How the mechanical microenvironment of stem cell growth affects their differentiation: a review

Cited by 27 publications

References 174 publications

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

A matrigel-free method for culture of pancreatic endocrine-like cells in defined protein-based hydrogels

Hypoxia Pathway in Osteoporosis: Laboratory Data for Clinical Prospects

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