How the Investigator Casework GO! Kit provides sensitive, fast and robust direct amplification of low copy number samples

Wolf, Kathrin; Pakulla-Dickel, Sarah; Río, Antonio Girela del; Prochnow, Anke; Elliott, Keith; Scherer, Mario

doi:10.1016/j.fsigss.2019.10.116

Cited by 3 publications

(8 citation statements)

References 1 publication

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“…Outside of the conventional high‐quality reference buccal and blood samples, the testing of direct PCR methods has been performed on various body fluids [43–50], hair [43,49,51], fingernails [52], tissues [49,53–55], touch DNA [45,46,49,56–59], and other evidentiary items [43,60]. Overall, the literature suggests that direct PCR methods may work better than traditional workflows for low‐yield samples.…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies testing these direct casework kits have demonstrated the successful genotyping of a wide variety of sample types, with comparable or improved results over traditional processing methods [44,45,59,77–79]. One such study performed by Sherier et al focused on the optimization of a modified direct PCR method using the Casework Direct Kit, Custom (Promega) for various dilutions of body fluid stains (blood, semen, saliva; neat‐1:100 dilution) on cloth [44].…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the major manufacturers must be preparing for this eminent switch to more direct methods for forensic evidence processing. A modified direct PCR approach resulting in a lysate not only provides a more efficient workflow for casework samples compared to traditional methods, but also retains the opportunity for quantifying a portion of the remaining DNA lysate that is produced [44,45,59], which complies with current FBI Quality Assurance Standards (QAS) requirements for casework samples [67].…”

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

“…for dilutions up to 1:1000, and reduced recovery for the 1:10,000 dilution samples (~20%-60% completeness) [45]. In general, more research involving direct PCR methods with mixed contributor samples and other challenging samples commonly submitted in SAKs is necessary to better inform the forensic community.…”

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

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Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing

Harrel¹,

Holmes²

2022

Journal of Forensic Sciences

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Crime laboratories have been faced with large casework backlogs due to lengthy processing times, limited resources and scientists, and rising crime rates. Evidence related to sexual assault crimes, specifically sexual assault kits (SAKs), heavily contribute to the reported backlogs. Although more sensitive, faster chemistries and automated techniques have been implemented over the years, the traditional STR workflow remains relatively unchanged. Enhanced workflows such as direct PCR and Rapid DNA

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

See 2 more Smart Citations

Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing

Harrel¹,

Holmes²

2022

Journal of Forensic Sciences

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show abstract

“…Having a fast analysis is of utmost importance at the scene of crime, for instance in the case of the identification of human remains or in sexual assault cases [6,7]. This is one of the motivations for the interest in microfluidic devices, which will be elaborated in the following section.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Approach for Biosensing DNA within Forensics

2020

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Reducing the risk of (cross-)contamination, improving the chain of custody, providing fast analysis times and options of direct analysis at crime scenes: these requirements within forensic DNA analysis can be met upon using microfluidic devices. To become generally applied in forensics, the most important requirements for microfluidic devices are: analysis time, method of DNA detection and biocompatibility of used materials. In this work an overview is provided about biosensing of DNA, by DNA profiling via standard short tandem repeat (STR) analysis or by next generation sequencing. The material of which a forensic microfluidic device is made is crucial: it should for example not inhibit DNA amplification and its thermal conductivity and optical transparency should be suitable for achieving fast analysis. The characteristics of three materials frequently used materials, i.e., glass, silicon and PDMS, are given, in addition to a promising alternative, viz. cyclic olefin copolymer (COC). New experimental findings are presented about the biocompatibility of COC and the use of COC chips for multiple displacement amplification and real-time monitoring of DNA amplification.

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Persistence of blood (DNA/RNA) on shoe soles under varying casework related conditions

Schmidt

Kunz

Wiegand

et al. 2022

Forensic Science International: Genetics

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How the Investigator Casework GO! Kit provides sensitive, fast and robust direct amplification of low copy number samples

Cited by 3 publications

References 1 publication

Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing

Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing

A Microfluidic Approach for Biosensing DNA within Forensics

Persistence of blood (DNA/RNA) on shoe soles under varying casework related conditions

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