How the bacterial plant pathogen <i>Xanthomonas campestris</i> pv. <i>vesicatoria</i> conquers the host

Bonas, Ulla; Ackerveken, Guido Van den; Büttner, Daniela; Hahn, Karoline; Marois, Eric; Nennstiel, Dirk; Noël, Laurent; Rossier, Ombeline; Szurek, Boris

doi:10.1046/j.1364-3703.2000.00010.x

Cited by 16 publications

(4 citation statements)

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“…Xe enters the plant tissue via natural openings, e.g., stomata, or wounds and multiplies locally in the intercellular space [ 14 ]. Pathogenicity of Xe relies on the type III-secretion system (T3SS), which is encoded by the chromosomal hrp (hypersensitive response and pathogenicity)-gene cluster [ 15 , 16 ] and translocates bacterial effector proteins (T3E) directly into the plant cell [ 17 ]. Expression of the T3SS components is induced during infection and in special minimal media (e.g., XVM2 [ 18 ]).…”

Section: Introductionmentioning

confidence: 99%

Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria

et al. 2017

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BackgroundBacteria of the genus Xanthomonas are economically important plant pathogens. Pathogenicity of Xanthomonas spp. depends on the type III-secretion system and additional virulence determinants. The number of sequenced Xanthomonas genomes increases rapidly, however, accurate annotation of these genomes is difficult, because it relies on gene prediction programs. In this study, we used a mass-spectrometry (MS)-based approach to identify the proteome of Xanthomonas euvesicatoria (Xe) strain 85–10 also known as X. campestris pv. vesicatoria, a well-studied member of plant-pathogenic Xanthomonadaceae.ResultsUsing different culture conditions, MS-datasets were searched against a six-frame-translated genome database of Xe. In total, we identified 2588 proteins covering 55% of the Xe genome, including 764 hitherto hypothetical proteins. Our proteogenomic approach identified 30 new protein-coding genes and allowed correction of the N-termini of 50 protein-coding genes. For five novel and two N-terminally corrected genes the corresponding proteins were confirmed by immunoblot. Furthermore, our data indicate that two putative type VI-secretion systems encoded in Xe play no role in bacterial virulence which was experimentally confirmed.ConclusionsThe discovery and re-annotation of numerous genes in the genome of Xe shows that also a well-annotated genome can be improved. Additionally, our proteogenomic analyses validates “hypothetical” proteins and will improve annotation of Xanthomonadaceae genomes, providing a solid basis for further studies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-4041-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria

et al. 2017

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“…The Xanthomonas genus is a ubiquitous group of plant‐associated bacterial pathogens that infect at least 124 monocotyledonous and 268 dicotyledonous plant species, many of which are economically important crops or plants (Bonas et al , ; Chan and Goodwin, ). A characteristic feature of the genus Xanthomonas is the production of yellow membrane‐bound pigments called xanthomonadins, which are mixtures of water‐insoluble, unusually brominated, aryl‐polyene esters (Starr and Stephens, ).…”

Section: Introductionmentioning

confidence: 99%

A novel 3‐oxoacyl‐ACP reductase (FabG3) is involved in the xanthomonadin biosynthesis of Xanthomonas campestris pv. campestris

Jianrong

Guo

et al. 2019

Molecular Plant Pathology

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Summary Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot in crucifers, produces a membrane‐bound yellow pigment called xanthomonadin to protect against photobiological and peroxidative damage, and uses a quorum‐sensing mechanism mediated by the diffusible signal factor (DSF) family signals to regulate virulence factors production. The Xcc gene XCC4003, annotated as Xcc fabG3, is located in the pig cluster, which may be responsible for xanthomonadin synthesis. We report that fabG3 expression restored the growth of the Escherichia coli fabG temperature‐sensitive mutant CL104 under non‐permissive conditions. In vitro assays demonstrated that FabG3 catalyses the reduction of 3‐oxoacyl‐acyl carrier protein (ACP) intermediates in fatty acid synthetic reactions, although FabG3 had a lower activity than FabG1. Moreover, the fabG3 deletion did not affect growth or fatty acid composition. These results indicate that Xcc fabG3 encodes a 3‐oxoacyl‐ACP reductase, but is not essential for growth or fatty acid synthesis. However, the Xcc fabG3 knock‐out mutant abolished xanthomonadin production, which could be only restored by wild‐type fabG3, but not by other 3‐oxoacyl‐ACP reductase‐encoding genes, indicating that Xcc FabG3 is specifically involved in xanthomonadin biosynthesis. Additionally, our study also shows that the Xcc fabG3‐disrupted mutant affects Xcc virulence in host plants.

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“…Xcv is the causal agent of bacterial spot disease on pepper and tomato, and has a completely different infectious cycle compared to R . solanacearum , entering the plant through stomata or wounds to reach the intercellular spaces 33 . Interestingly, we provide evidence of a functional complementation of the R .…”

Section: Introductionmentioning

confidence: 99%

HpaB-Dependent Secretion of Type III Effectors in the Plant Pathogens Ralstonia solanacearum and Xanthomonas campestris pv. vesicatoria

Lonjon

Lohou

Cazalé

et al. 2017

Sci Rep

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Plant pathogenic bacteria exerts their pathogenicity through the injection of large repertoires of type III effectors (T3Es) into plant cells, a mechanism controlled in part by type III chaperones (T3Cs). In Ralstonia solanacearum, the causal agent of bacterial wilt, little is known about the control of type III secretion at the post-translational level. Here, we provide evidence that the HpaB and HpaD proteins do act as bona fide R. solanacearum class IB chaperones that associate with several T3Es. Both proteins can dimerize but do not interact with each other. After screening 38 T3Es for direct interactions, we highlighted specific and common interacting partners, thus revealing the first picture of the R. solanacearum T3C-T3E network. We demonstrated that the function of HpaB is conserved in two phytopathogenic bacteria, R. solanacearum and Xanthomonas campestris pv. vesicatoria (Xcv). HpaB from Xcv is able to functionally complement a R. solanacearum hpaB mutant for hypersensitive response elicitation on tobacco plants. Likewise, Xcv is able to translocate a heterologous T3E from R. solanacearum in an HpaB-dependent manner. This study underlines the central role of the HpaB class IB chaperone family and its potential contribution to the bacterial plasticity to acquire and deliver new virulence factors.

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How the bacterial plant pathogen Xanthomonas campestris pv. vesicatoria conquers the host

Cited by 16 publications

References 23 publications

Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria

Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria

A novel 3‐oxoacyl‐ACP reductase (FabG3) is involved in the xanthomonadin biosynthesis of Xanthomonas campestris pv. campestris

HpaB-Dependent Secretion of Type III Effectors in the Plant Pathogens Ralstonia solanacearum and Xanthomonas campestris pv. vesicatoria

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