How reliable is dietary supplement labelling?—Experiences from the analysis of ecdysterone supplements

Ambrosio, Gabriella; Wirth, David D.; Joseph, Jan Felix; Mazzarino, Monica; Torre, Xavier de la; Botrè, Francesco; Parr, Maria Kristina

doi:10.1016/j.jpba.2019.112877

Cited by 18 publications

(14 citation statements)

References 23 publications

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“…The recently reported study of Ambrosio et al 14 showed variability of the 20‐OHE concentration in 12 supplements tested and also mentioned the fact that the concentration of 20‐OHE was often significantly lower than claimed on the label. The current study confirmed that finding, albeit in different supplements.…”

Section: Resultsmentioning

confidence: 93%

“…The detection of 20‐OHE and its metabolites after administration of high doses in human volunteers (20 and 50 mg) has been reported by Tsitsimpikou et al 10 and Parr et al, 11 respectively. However, the doses present in the supplements vary considerably and often do not accurately reflect those on the label 14 . Therefore, there is a need to quantitate the 20‐OHE levels in the supplements themselves, as well as, detect the fate, both parent and metabolite, after in vivo ingestion.…”

Section: Introductionmentioning

confidence: 99%

“…However, the doses present in the supplements vary considerably and often do not accurately reflect those on the label. 14 Therefore, there is a need to quantitate the 20‐OHE levels in the supplements themselves, as well as, detect the fate, both parent and metabolite, after in vivo ingestion.…”

Section: Introductionmentioning

confidence: 99%

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Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)‐SCID mice with humanized liver, human urine samples, and estimation of prevalence of its use in anti‐doping samples

Kraiem

Aljaber

Al-Mohammed

et al. 2021

Drug Testing and Analysis

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Ecdysteroids are of interest as potential sport performance enhancers, due to their anabolic effects. The current study aimed to analyze levels of the most abundant ecdysteroid, ecdysterone (20-hydroxyecdysone, 20-OHE) in easily available dietary supplements, and, outline an analytical strategy for its detection, and that, of its metabolites, (1) following administration of pure 20-OHE to uPA(+/+)-SCID mice with humanized liver, (2) in a human volunteer after ingestion of two supplements, one with a relatively low, and the other a high, concentration of 20-OHE, and, (3) to estimate the prevalence of use of 20-OHE in elite athletes (n = 1000). Of the 16 supplements tested, only five showed detectable levels of 20-OHE, with concentrations ranging from undetectable up to 2.3 mg per capsule. Urine of uPA(+/+)-SCID urine showed the presence of 20-OHE and its metabolite, 14 deoxy ecdysterone, within 24 hours (hr) of ingestion. In humans, both the parent and the metabolite were detectable within 2 to 5 hr of ingestion, with the metabolite being detectable for longer than the parent. After ingestion of a low dose supplement, the parent and metabolite were detectable for 70 and 48 hr, while following the higher dose it was 96 and 48 hr, respectively. Analysis of urines from athletes (n = 1000) confirmed four positives for 20-OHE, suggesting a prevalence of use of 0.4%.

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)‐SCID mice with humanized liver, human urine samples, and estimation of prevalence of its use in anti‐doping samples

Kraiem

Aljaber

Al-Mohammed

et al. 2021

Drug Testing and Analysis

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“…Early experimental studies and clinical trials on humans have indicated that 20E or plant extracts containing significant amounts of ecdysteroids [104]: A major aim of early human studies was to improve physical performances of healthy young people, and they showed a significant improvement in muscle mass and/or endurance after a few days/weeks. Such experiments paved the way, directly or indirectly, for use as a "doping substance" by sportsmen or bodybuilders based on the consumption of uncontrolled dietary supplements [162]. The recent confirmation of these anabolic results [163] led the authors to propose the inscription of 20E on the list of doping prohibited substances [164].…”

Section: Clinical Studiesmentioning

confidence: 99%

20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

et al. 2021

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There is growing interest in the pharmaceutical and medical applications of 20-hydroxyecdysone (20E), a polyhydroxylated steroid which naturally occurs in low but very significant amounts in invertebrates, where it has hormonal roles, and in certain plant species, where it is believed to contribute to the deterrence of invertebrate predators. Studies in vivo and in vitro have revealed beneficial effects in mammals: anabolic, hypolipidemic, anti-diabetic, anti-inflammatory, hepatoprotective, etc. The possible mode of action in mammals has been determined recently, with the main mechanism involving the activation of the Mas1 receptor, a key component of the renin–angiotensin system, which would explain many of the pleiotropic effects observed in the different animal models. Processes have been developed to produce large amounts of pharmaceutical grade 20E, and regulatory preclinical studies have assessed its lack of toxicity. The effects of 20E have been evaluated in early stage clinical trials in healthy volunteers and in patients for the treatment of neuromuscular, cardio-metabolic or respiratory diseases. The prospects and limitations of developing 20E as a drug are discussed, including the requirement for a better evaluation of its safety and pharmacological profile and for developing a production process compliant with pharmaceutical standards.

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“…Uno de los estudios seleccionados mostró que 6 de los 15 SN analizados incluían detalles sobre el contenido de cafeína en el etiquetado, y el porcentaje hallado tenía una variación de entre el 59 % y el 176 % con respecto al declarado en el panel nutricional (24). Este tipo de negligencia no es exclusiva de los SN, ya que se ha evidenciado también en otros tipos de productos como son los complementos dietéticos, los alimentos y los productos alimentarios (11,17,32). Además, en los estudios revisados, la diferencia entre el contenido real y el declarado existía tanto por exceso como por defecto (18,20,21).…”

Section: Discussionunclassified

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SENPE¹

2018

Nutr Hosp

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How reliable is dietary supplement labelling?—Experiences from the analysis of ecdysterone supplements

Cited by 18 publications

References 23 publications

Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)‐SCID mice with humanized liver, human urine samples, and estimation of prevalence of its use in anti‐doping samples

Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)‐SCID mice with humanized liver, human urine samples, and estimation of prevalence of its use in anti‐doping samples

20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases

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