How plants solubilise seed fats: revisiting oleosin structure and function to inform commercial applications

Board, A.J.; Crowther, Jennifer M.; Acevedo-Fani, Alejandra; Meisrimler, Claudia-Nicole; Jameson, Geoffrey B.; Dobson, Renwick C. J.

doi:10.1007/s12551-021-00923-5

Cited by 18 publications

(7 citation statements)

References 84 publications

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“…The expression of DGAT with an engineered plant structural protein (cysteine [Cys]‐oleosin) was shown to result in TAG accumulation in Arabidopsis (Winichayakul et al ., 2013 ). Suppression of lipid degradation can be achieved by overexpression of OLEOSIN (OLE), a structural protein that coats oil bodies in developing seeds and other tissues (Board et al ., 2022 ; Chapman et al ., 2012 ). In combination, overexpression of Arabidopsis WRINKLED1 ( AtWRI1 ), Arabidopsis DGAT1 ( AtDGAT1 ), and sesame OLEOSIN1 ( SiOLE ) have resulted in levels of TAG up to 15% of DW in tobacco leaves (Vanhercke et al ., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Engineering triacylglycerol accumulation in duckweed (Lemna japonica)

Liang

Anaokar

et al. 2022

Plant Biotechnology Journal

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Duckweeds are amongst the fastest growing of higher plants, making them attractive highbiomass targets for biofuel feedstock production. Their fronds have high rates of fatty acid synthesis to meet the demand for new membranes, but triacylglycerols (TAG) only accumulate to very low levels. Here we report the engineering of Lemna japonica for the synthesis and accumulation of TAG in its fronds. This was achieved by expression of an estradiol-inducible cyan fluorescent protein-Arabidopsis WRINKLED1 fusion protein (CFP-AtWRI1), strong constitutive expression of a mouse diacylglycerol:acyl-CoA acyltransferase2 (MmDGAT), and a sesame oleosin variant (SiOLE(*)). Individual expression of each gene increased TAG accumulation by 1-to 7-fold relative to controls, while expression of pairs of these genes increased TAG by 7-to 45-fold. In uninduced transgenics containing all three genes, TAG accumulation increased by 45fold to 3.6% of dry weight (DW) without severely impacting growth, and by 108-fold to 8.7% of DW after incubation on medium containing 100 μM estradiol for four days. TAG accumulation was accompanied by an increase in total fatty acids of up to 3-fold to approximately 15% of DW. Lipid droplets from fronds of all transgenic lines were visible by confocal microscopy of BODIPY-stained fronds. At a conservative 12 tonnes (dry matter) per acre and 10% (DW) TAG, duckweed could produce 350 gallons of oil/acre/year, approximately 7-fold the yield of soybean, and similar to that

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Section: Introductionmentioning

confidence: 99%

Engineering triacylglycerol accumulation in duckweed (Lemna japonica)

Liang

Anaokar

et al. 2022

Plant Biotechnology Journal

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“…A0A1S2XVM2, Mr of 35 kDa, at a relative abundance of 23%) were the most abundant proteins in band N. 15 (with an apparent Mr of 30 kDa). The oil body-associated protein belongs to a highly conserved plant group of proteins (i.e., oleosin) that are important for solubilizing seed fats and playing an important role in regulating the biosynthesis, metabolization, and mobilization of lipids during seed maturation and germination [40,41]. Two group members of the late embryogenesis abundant protein family (i.e., a dehydrin DHN3: Acc.…”

Section: The Most Abundant Proteins Identified In Each Sds-page Bandmentioning

confidence: 99%

Mass Spectrometry Characterization of the SDS-PAGE Protein Profile of Legumins and Vicilins from Chickpea Seed

Di Francesco,

De Santis,

Lanzoni

et al. 2024

Foods

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Chickpea (Cicer arietinum L.) seed proteins show a lot of functional properties leading this legume to be an interesting component for the development of protein-enriched foods. However, both the in-depth proteomic investigation and structural characterization of chickpea seed proteins are still lacking. In this paper a detailed characterization of chickpea seed protein fraction by means of SDS-PAGE, in-gel protein digestion, high-resolution mass spectrometry, and database searching is reported. Through this approach, twenty SDS gel bands were cut and analyzed. While the majority of the bands and the identified peptides were related to vicilin and legumin storage proteins, metabolic functional proteins were also detected. Legumins, as expected, were revealed at 45–65 kDa, as whole subunits with the α- and β-chains linked together by a disulphide bond, but also at lower mass ranges (α- and β-chains migrating alone). Similarly, but not expected, the vicilins were also spread along the mass region between 65 and 23 kDa, with some of them being identified in several bands. An MS structural characterization allowed to determine that, although chickpea vicilins were always described as proteins lacking cysteine residues, they contain this amino acid residue. Moreover, similar to legumins, these storage proteins are firstly synthesized as pre-propolypeptides (Mr 50–80 kDa) that may undergo proteolytic steps that not only cut the signal peptides but also produce different subunits with lower molecular masses. Overall, about 360 different proteins specific of the Cicer arietinum L. species were identified and characterized, a result that, up to the current date, represents the most detailed description of the seed proteome of this legume.

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“…In addition, their content of proline enhances the protein's stability and safeguards it from desiccation stress. Another example of genes that encode storage protein are oleosin genes in oilseeds, such as rapeseed, sunflower seeds, and sesame seeds [85]. These genes are associated with lipid bodies and play a role in storing and safeguarding oil reserves during seed desiccation.…”

Section: Gene Alteration In Seeds During Desiccationmentioning

confidence: 99%

An Insight into the Behaviour of Recalcitrant Seeds by Understanding Their Molecular Changes upon Desiccation and Low Temperature

et al. 2023

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Systems biology is an interdisciplinary study that involves a combination of expertise in biology, chemistry, mathematics, physics, and engineering to unravel the biology of complex living systems by incorporating multiple kinds of quantitative molecular computations by using sophisticated mathematical models. This interdisciplinary study can be applied to identify and understand molecular and metabolic changes in recalcitrant plant species. Many tropical plants with recalcitrant seeds have difficulty with long-term seed storage and preservation due to their intolerance to desiccation and low temperatures. The aim of this review was to explore and discuss how omics analyses can assist in elucidating molecular responses and metabolic changes of recalcitrant seed species. Genomics and transcriptomics analyses identified genes, such as late embryogenesis abundant (LEA), that were highly expressed after exposure to desiccation and low temperatures. Meanwhile, proteomic analysis using 2D gel electrophoresis, MALDI-TOF MS, or MS/MS analysis revealed dehydrins induced from recalcitrant seeds upon exposure to desiccation and low temperatures. Metabolomic analysis using liquid chromatography–mass spectrometry (LC–MS) and gas chromatography–mass spectrometry (GC–MS) profiling of recalcitrant seeds has discovered metabolites such as sugar and organic acid changes in recalcitrant seeds at different developmental stages. This information may contribute to comprehending the behaviour of recalcitrant seeds and provide insight into how crop management can be improved in terms of seed storage for conservation in order to maintain plant biodiversity.

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How plants solubilise seed fats: revisiting oleosin structure and function to inform commercial applications

Cited by 18 publications

References 84 publications

Engineering triacylglycerol accumulation in duckweed (Lemna japonica)

Engineering triacylglycerol accumulation in duckweed (Lemna japonica)

Mass Spectrometry Characterization of the SDS-PAGE Protein Profile of Legumins and Vicilins from Chickpea Seed

An Insight into the Behaviour of Recalcitrant Seeds by Understanding Their Molecular Changes upon Desiccation and Low Temperature

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