How peptide physicochemical and structural characteristics affect anion-exchange membranes fouling by a tryptic whey protein hydrolysate

Fouling prevention of peptides from a tryptic whey hydrolysate during electromembrane processes by use of monovalent ion permselective membranes

“…At pH 6, the TPEVDDEALEK, TPEVDDEALEKFDK and VLVDTDYK sequences represented about 78 and 83 % of the total abundance for the AMX-SB and the MAP membranes, respectively. These peptides carried negatively charged D and E residues (pKa=4.0) which could interact electrostatically with the membrane positive charges (Table 7) as previously explained in details for anion-exchange membranes by Persico et al 9 according to the physicochemical properties of the peptides, the amino acid place into the peptide sequence and the 3D structure of the peptides.…”

“…The same protocol was carried-out for MCP membranes. The peptide fractions previously obtained for AMX-SB 9 and CMX-SB 10 membranes were re-analysed for peptide identification in the same conditions and with the same equipment as MAP and MCP to be able to compare the UPLC-QTOF results. Figure 1) as in 11 .…”

“…Recent studies showed that conventional food-grade anion-exchange membranes (AMX-SB) and/or cation-exchange membranes (CMX-SB) are exposed to fouling in presence of a WPH either in static 9,10 or in hydrodynamic conditions during an ED process 11 . Fouling was mainly due to a few peptides which could establish electrostatic interactions with the membranes, and that even with no current applied.…”

“…(3) to compare that fouling in terms of quantity and peptide sequences with the conventional membranes from previous studies 9,10,11 carried-out with the same WPH and in the same static and ED conditions.…”

Recent insights into structure-function relationships of antimicrobial peptides

“…Besides, large‐scale fractionation and purification of AMPs still a challenge for the industry, with fouling of separation membrane being the most frequent problem. Recently, peptide aggregation to anion and cation‐exchange membranes was reported to involve electrostatic interactions and to be influenced by pH of the solution and charges distribution at the surface of the macromolecular structures (Persico et al, ).…”