How Osmolytes Modulate Lipid Interactions

Abstract

To counteract environmental stress, many organisms regulate the amounts of molecularly small solutes termed osmolytes. Recent evidences show that osmolytes alter lipid homeostasis, with potential consequences to cardiovascular disease.[1] Although it is known that many osmolytes exert an apparent attractive or repulsive force between self-assembled lipid membranes, all proposed models fail to fully account for the origin of this force. Toward resolving the mechanism by which osmolytes modulate lipid interactions, we followed the quintessential osmolyte trimethylamine N-oxide (TMAO) and its interaction with dimyristoyl phosphatidylcholine (DMPC) membranes in aqueous solution.[2] We found that TMAO pushes adjacent membranes closer together. By contrast, our newly formulated methodologies for determining membrane elasticity from simulations [3,4] show almost no change in membrane structural and material properties in presence of TMAO. Experiments and simulations further show that the change in the force between membranes is due to the partitioning of TMAO away from the volume between bilayers. This in turn stems from the exclusion of TMAO from the lipid-water interface. Hence, the underlying mechanism resembles protein stabilization by osmolytes. We extend our conclusions to address additional biologically relevant solutes (e.g., sugars, DMSO and urea), explaining how osmolytes can increase membrane-membrane attraction or repulsion depending on their preferential interactions with lipids. Finally, we discuss the potential synergistic role of osmolytes acting together with inorganic salts, including calcium, in the modifications of lipid adhesion and fusion interactions.