How nitrification-related N2O is associated with soil ammonia oxidizers in two contrasting soils in China?

Yang, Liuqing; Zhu, Gaodi; Ju, Xiaotang; Li, Rui

doi:10.1016/j.scitotenv.2020.143212

Cited by 26 publications

(10 citation statements)

References 82 publications

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“…Studies have shown that soil N availability and pH are key factors in constructing AOA and AOB communities, AOA prefers less ammonia and acidic conditions, while AOB prefers nitrogen-rich alkaline conditions [ 19 , 42 ]. In addition, temperature [ 44 ] and O 2 [ 45 ] are also effective factors affecting AOA and AOB communities. The oxidation of ammonia is traditionally thought to be carried out by AOB, so N-based fertilization may be a primary driver for AOB [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in Ammonia-Oxidizing Archaea and Bacterial Communities and Soil Nitrogen Dynamics in Response to Long-Term Nitrogen Fertilization

Xie

et al. 2022

IJERPH

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Ammonia oxidizing archaea (AOA) and bacteria (AOB) mediate a crucial step in nitrogen (N) metabolism. The effect of N fertilizer rates on AOA and AOB communities is less studied in the wheat-fallow system from semi-arid areas. Based on a 17-year wheat field experiment, we explored the effect of five N fertilizer rates (0, 52.5, 105, 157.5, and 210 kg ha−1 yr−1) on the AOA and AOB community composition. This study showed that the grain yield of wheat reached the maximum at 105 kg N ha−1 (49% higher than control), and no further significant increase was observed at higher N rates. With the increase of N, AOA abundance decreased in a regular trend from 4.88 × 107 to 1.05 × 107 copies g−1 dry soil, while AOB abundance increased from 3.63 × 107 up to a maximum of 8.24 × 107 copies g−1 dry soil with the N105 treatment (105 kg N ha−1 yr−1). Application rates of N fertilizer had a more significant impact on the AOB diversity than on AOA diversity, and the highest AOB diversity was found under the N105 treatment in this weak alkaline soil. The predominant phyla of AOA and AOB were Thaumarchaeota and Proteobacteria, respectively, and higher N treatment (N210) resulted in a significant decrease in the relative abundance of genus Nitrosospira. In addition, AOA and AOB communities were significantly associated with grain yield of wheat, soil potential nitrification activity (PNA), and some soil physicochemical parameters such as pH, NH4-N, and NO3-N. Among them, soil moisture was the most influential edaphic factor for structuring the AOA community and NH4-N for the AOB community. Overall, 105 kg N ha−1 yr−1 was optimum for the AOB community and wheat yield in the semi-arid area.

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Section: Introductionmentioning

confidence: 99%

Changes in Ammonia-Oxidizing Archaea and Bacterial Communities and Soil Nitrogen Dynamics in Response to Long-Term Nitrogen Fertilization

Xie

et al. 2022

IJERPH

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“…Soil temperature is another key factor controlling NI efficiency, which can subside after 1 week at 35°C ( Barth et al, 2008 ; Chen et al, 2015 ). Furthermore, many studies focused on the impact and contribution of soil microorganisms on N 2 O emissions ( Chen et al, 2019 ; Lazcano et al, 2021 ; Yang et al, 2021 ; You et al, 2022 ). However, there is still a lack of direct evidence on whether soil microbial community, especially AOA and AOB, affects NI efficacy ( Chen et al, 2010 ; Guardia et al, 2018 ; Lam et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

A meta-analysis to examine whether nitrification inhibitors work through selectively inhibiting ammonia-oxidizing bacteria

Lei

Fan²,

Yu³

et al. 2022

Front. Microbiol.

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Nitrification inhibitor (NI) is often claimed to be efficient in mitigating nitrogen (N) losses from agricultural production systems by slowing down nitrification. Increasing evidence suggests that ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) have the genetic potential to produce nitrous oxide (N2O) and perform the first step of nitrification, but their contribution to N2O and nitrification remains unclear. Furthermore, both AOA and AOB are probably targets for NIs, but a quantitative synthesis is lacking to identify the “indicator microbe” as the best predictor of NI efficiency under different environmental conditions. In this present study, a meta-analysis to assess the response characteristics of AOB and AOA to NI application was conducted and the relationship between NI efficiency and the AOA and AOB amoA genes response under different conditions was evaluated. The dataset consisted of 48 papers (214 observations). This study showed that NIs on average reduced 58.1% of N2O emissions and increased 71.4% of soil NH4+ concentrations, respectively. When 3, 4-dimethylpyrazole phosphate (DMPP) was applied with both organic and inorganic fertilizers in alkaline medium soils, it had higher efficacy of decreasing N2O emissions than in acidic soils. The abundance of AOB amoA genes was dramatically reduced by about 50% with NI application in most soil types. Decrease in N2O emissions with NI addition was significantly correlated with AOB changes (R2 = 0.135, n = 110, P < 0.01) rather than changes in AOA, and there was an obvious correlation between the changes in NH4+ concentration and AOB amoA gene abundance after NI application (R2 = 0.037, n = 136, P = 0.014). The results indicated the principal role of AOB in nitrification, furthermore, AOB would be the best predictor of NI efficiency.

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“…We found that AOB played a more important role than AOA in ammonia oxidation in a high-pH soil, supporting previous reports 21 , 22 , 41 , 42 . One possible explanation is that soils with higher pH accelerate the rate transformation of NH 4 + –N to availability NH 3 which affected population and activity of ammonia oxidizers 27 , while bacterial growth would possibly have been impeded in low soil pH 43 .…”

Section: Discussionmentioning

confidence: 99%

“…Wang et al 21 reported that nitrogen fertiliser-induced N 2 O emissions are attributed 70.5 ~ 78.1% by AOB and 18.7 ~ 19.7% by AOA using the method of inhibitors both in acidic (pH = 6) and alkaline (pH = 8) arable soils of China. Similarly, using the method of inhibitors, Yang et al 27 found that AOB was the key microbial player in alkaline soil which contributing about 85% of nitrification-related N 2 O, while 78% of nitrification-related N 2 O was contributed by AOA in acidic soil. In addition, the relative contribution of AOB and AOA to N 2 O emissions was also regulated by type and amount of applied synthetic Hink et al 24 found that high ammonia addition stimulated the production of N 2 O from AOB, but AOA dominance during low ammonium supply.…”

Section: Introductionmentioning

confidence: 99%

The contributions of ammonia oxidizing bacteria and archaea to nitrification-dependent N2O emission in alkaline and neutral purple soils

Dong

Wang

et al. 2022

Sci Rep

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Nitrification is believed to be one of the primary processes of N2O emission in the agroecological system, which is controlled by soil microbes and mainly regulated by soil pH, oxygen content and NH4+ availability. Previous studies have proved that the relative contributions of ammonia oxidizing bacteria (AOB) and archaea (AOA) to N2O production were varied with soil pH, however, there is still no consensus on the regulating mechanism of nitrification-derived N2O production by soil pH. In this study, 1-octyne (a selective inhibitor of AOB) and acetylene (an inhibitor of AOB and AOA) were used in a microcosm incubation experiment to differentiate the relative contribution of AOA and AOB to N2O emissions in a neutral (pH = 6.75) and an alkaline (pH = 8.35) soils. We found that the amendment of ammonium (NH4+) observably stimulated the production of both AOA and AOB-related N2O and increased the ammonia monooxygenase (AMO) gene abundances of AOA and AOB in the two test soils. Among which, AOB dominated the process of ammonia oxidation in the alkaline soil, contributing 70.8% of N2O production derived from nitrification. By contrast, the contribution of AOA and AOB accounted for about one-third of nitrification-related N2O in acidic soil, respectively. The results indicated that pH was a key factor to change abundance and activity of AOA and AOB, which led to the differentiation of derivation of N2O production in purple soils. We speculate that both NH4+ content and soil pH mediated specialization of ammonia-oxidizing microorganisms together; and both specialization results and N2O yield led to the different N2O emission characteristics in purple soils. These results may help inform the development of N2O reduction strategies in the future.

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How nitrification-related N2O is associated with soil ammonia oxidizers in two contrasting soils in China?

Cited by 26 publications

References 82 publications

Changes in Ammonia-Oxidizing Archaea and Bacterial Communities and Soil Nitrogen Dynamics in Response to Long-Term Nitrogen Fertilization

Changes in Ammonia-Oxidizing Archaea and Bacterial Communities and Soil Nitrogen Dynamics in Response to Long-Term Nitrogen Fertilization

A meta-analysis to examine whether nitrification inhibitors work through selectively inhibiting ammonia-oxidizing bacteria

The contributions of ammonia oxidizing bacteria and archaea to nitrification-dependent N2O emission in alkaline and neutral purple soils

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