How myristyltrimethylammonium bromide enhances biomass harvesting and pigments extraction from Synechocystis sp. PCC 6803

Zhou, Yun; Lai, YenJung Sean; Eustance, Everett; Straka, Levi; Zhou, Chen; Xia, Siqing; Rittmann, Bruce E.

doi:10.1016/j.watres.2017.09.036

Cited by 25 publications

(37 citation statements)

References 42 publications

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“…With a higher CAPB dosage (>0.10 g/g), the system pH value decreased in the initial 2 h and a high CAPB dosage led to a lower pH value, which might result from the cell lysis and the release of intracellular NA after addition of a high dose of surfactant. 11,23 Thereafter, system pH value kept increasing and showed a similar trend when a low dose of CAPB was added to the system.…”

Section: ■ Results and Discussionmentioning

confidence: 80%

“…With addition of CAPB from 0 to 0.10 g/g, the system pH value gradually increased and tended to be alkalescent throughout the STAD process, which was consistent with previous studies. , Additionally, a high dose of CAPB caused a higher system pH value but then remained stable at about 7.8 when CAPB dosage increased from 0.08 to 0.10 g/g. With a higher CAPB dosage (>0.10 g/g), the system pH value decreased in the initial 2 h and a high CAPB dosage led to a lower pH value, which might result from the cell lysis and the release of intracellular NA after addition of a high dose of surfactant. , Thereafter, system pH value kept increasing and showed a similar trend when a low dose of CAPB was added to the system.…”

Section: Resultsmentioning

confidence: 93%

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Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Zhou

Zhang

et al. 2018

ACS Sustainable Chem. Eng.

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Cocoamidopropyl betaine (CAPB) has newly been found to improve the reduction of waste-activated sludge (WAS) by a short-time aerobic digestion (STAD) process. This work systematically discloses the influences of CAPB dosage on the reduction of WAS by the STAD process. Results showed that CAPB lower than 0.10 g/g of TSS (total suspended solids) mainly increase the organic substances release and low-molecularweight (MW) fractions proportion via its surfactant action, leading to the increased concentration of soluble biopolymers. The concentration of soluble PO 4 3− -P and NH 4 + -N increased during the biopolymers release, but gradually decreased in the later stage. Moreover, the specific oxygen uptake rate (SOUR) of digested sludge (denoted as aerobic microorganisms activity) was increased, resulting in the increased biodegradation rate of WAS chemical oxygen demand (k COD,WAS ). A higher amount of CAPB (>0.10 g/g of TSS) led to cell lysis and intracellular polymeric substances (IPS) release, which led to an additional increase of PO 4 3− -P, NH 4 + -N, soluble organic matters, and its low MW fractions; all these fractions gradually decreased later. Nevertheless, cell lysis reduces both pH value and SOUR, which caused the decrease of CAPB biodegradation rate and k COD,WAS . This study lays the foundation for improving the reduction efficiency of WAS in the STAD system by optimizing surfactant dose.

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Section: ■ Results and Discussionmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 93%

Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Zhou

Zhang

et al. 2018

ACS Sustainable Chem. Eng.

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“…[16] Catalytic efficacy is determined by optimization of molecular structure (charge and structure of amphiphilic molecule head group and chain length [17][18][19] ), concentration and type of self-assemblies (micelles, rods, vesicles etc.). [20][21][22][23] Cationic surfactants are the most promising due to the charge of head group for biological applications, [3] environmental issues (extractants and flocculants) [24][25][26] and as catalysts in nucleophilic substitution reaction for hydrolysis of ester bonds. [17,27,28] Reactions of nucleophilic substitution in phosphorus esters play an important role in biological processes.…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembled Quaternary Ammonium‐Containing Comb‐Like Polyelectrolytes for the Hydrolysis of Organophosphorous Esters: Effect of Head Groups and Counter‐Ions

et al. 2020

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The aim of this work was to increase the efficiency of catalytic systems for the hydrolytic cleavage of 4‐nitrophenyl esters of phosphonic acids. Quaternary ammonium‐containing comb‐like polyelectrolytes («polymerized micelles») with ester cleavable fragments and a low aggregation threshold were used as catalysts. The synthesis of poly(11‐acryloyloxyundecylammonium) surfactants with different counterions (Br−, NO3−, CH3C6H4SO3−) and head groups was realized by micellar free‐radical polymerization. Molecular weight, critical association concentration, particle sizes and solubilization properties toward Orange OT were determined. Self‐assemblies organized by poly(11‐acryloyloxyundecyltrimethyl ammonium) bromide successfully catalyze the hydrolysis of 4‐nitrophenyl butylchloromethylphosphonate up to two orders of magnitude compared to aqueous alkaline hydrolysis. The development of these catalysts is promising for industrial applications and organophosphorus compound detoxification.

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“…Like most bacteria, Synechocystis produces extracellular polymeric substances (EPS) that it uses for aggregation and protection against environmental toxicants . EPS, which can comprise as much as 8.4% of the dry weight of Synechocystis , contain carboxyl (≡X-COOH) and phosphoryl (≡X-PO 4 H) groups that are negatively charged at slightly acidic to alkaline conditions. − Due to its small cell size and negative charge from EPS, Synechocystis does not readily self-flocculate, and the biomass-harvesting process incurs high cost. Thus, removing EPS to reduce the cells’ negative charge may improve biomass harvesting.…”

Section: Introductionmentioning

confidence: 99%

“…Among a number of approaches for enhancing the aggregation of biomass, , adding cationic surfactants has particular promise in this context. ,− When the surfactant is adsorbed to cells, its quaternary-ammonium cation makes the cell’s charge less negative, which enhances the onset of aggregation . The long alkyl chain is an interparticle bridge that links the cells together, which further enhances aggregation.…”

Section: Introductionmentioning

confidence: 99%

Promoting Synechocystis sp. PCC 6803 Harvesting by Cationic Surfactants: Alkyl-Chain Length and Dose Control for the Release of Extracellular Polymeric Substances and Biomass Aggregation

Zhou

Lai

Eustance

et al. 2018

ACS Sustainable Chem. Eng.

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Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactantshexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB)affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91%. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.

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How myristyltrimethylammonium bromide enhances biomass harvesting and pigments extraction from Synechocystis sp. PCC 6803

Cited by 25 publications

References 42 publications

Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Self‐Assembled Quaternary Ammonium‐Containing Comb‐Like Polyelectrolytes for the Hydrolysis of Organophosphorous Esters: Effect of Head Groups and Counter‐Ions

Promoting Synechocystis sp. PCC 6803 Harvesting by Cationic Surfactants: Alkyl-Chain Length and Dose Control for the Release of Extracellular Polymeric Substances and Biomass Aggregation

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