How much separation for LC–MS/MS quantitative bioanalysis of drugs and metabolites?

Tan, Aimin; Fanaras, John C.

doi:10.1016/j.jchromb.2018.03.019

Cited by 7 publications

(6 citation statements)

References 43 publications

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“…Several authors have identified polyphenols in cladodes through HPLC and UHPLC because they maximize polyphenol identification accuracy (Tan & Fanaras, 2018). Hence, these techniques lead the separation methods for polyphenols analysis (Table 3).…”

Section: Identification Of Polyphenolsmentioning

confidence: 99%

Cladodes: Chemical and structural properties, biological activity, and polyphenols profile

Perucini-Avendaño¹,

Nicolás-García²,

Jiménez‐Martínez³

et al. 2021

Food Science & Nutrition

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The nopal cactus is an essential part of the Mexican diet and culture. The per capita consumption of young cladodes averages annually to 6.4 kg across the nation. In addition to contributing to the country's food culture, the nopal is considered a food with functional characteristics since, in addition to providing fiber, an important group of polyphenolic compounds is present, which has given cladodes to be considered a healthy food, for what they have been incorporated into the diet of Mexican people and many other countries worldwide. Research suggests that polyphenols from cladodes act as antioxidants and antidiabetics. This review studies the main phenolic components in cladodes and summarizes both conventional and novel methods to identify them.

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Section: Identification Of Polyphenolsmentioning

confidence: 99%

Cladodes: Chemical and structural properties, biological activity, and polyphenols profile

Perucini-Avendaño¹,

Nicolás-García²,

Jiménez‐Martínez³

et al. 2021

Food Science & Nutrition

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“…The elution of interfering compounds or matrix effect (ion suppression or enhancement) can be greatly affected by the pH of mobile phases (Chambers, Wagrowski‐Diehl, Lu, & Mazzeo, ; Rainville et al, ; Ruta et al, ; Tan & Fanaras, ). Hence, the interfering compounds and matrix effect pattern by using a basic mobile phase could be completely different from those of acidic mobile phases.…”

Section: Benefits Of Using High‐ph Mobile Phasesmentioning

confidence: 99%

“…Finally, as mentioned earlier, the interference compounds or matrix effect compounds are mobile phase specific (Tan & Fanaras, ). Sometimes it can be very challenging to separate a matrix effect or interfering peak from the compounds of interest with a basic mobile phase.…”

Section: Cautions For Using High‐ph Mobile Phasesmentioning

confidence: 99%

Use of high‐pH (basic/alkaline) mobile phases for LC–MS or LC–MS/MS bioanalysis

Tan

Fanaras

2018

Biomedical Chromatography

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High-pH or basic/alkaline mobile phases are not commonly used in LC-MS or LC-MS/MS bioanalysis because of the deeply rooted concern with column instability and reduced detection sensitivity for basic compounds in high-pH mobile phases owing to charge neutralization. With the advancement of LC column technology and the wide recognition of the "wrong-way-round" phenomena, high-pH mobile phases are more and more used in LC-MS or LC-MS/MS bioanalysis to improve chromatographic peak shape, retention, selectivity, resolution, and detection sensitivity, not only for basic compounds, but also for many other compounds. In this article, the benefits, practical considerations, application examples and cautions for using high-pH mobile phases in LC-MS or LC-MS/MS bioanalysis are reviewed, with a focus on quantification. Furthermore, the future trends in this field are also envisaged.A total of 84 references are cited in this review. KEYWORDS alkaline mobile phase, basic mobile phase, bioanalysis, high pH, LC-MS, LC-MS/MS

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“…Detection in HPLC includes UV–vis absorption, fluorescence, refractive index, chemiluminescence, various types of electrochemical detection, evaporative light‐scattering, etc., offering a wide range of possibilities for measuring the analytes or their derivatives. HPLC coupled with mass spectrometry (MS) brings analytical advantages for selectivity and sensitivity (Borden et al, 2020), although the literature also debates some limitations (Medvedovici, Albu, & David, 2010) or the magnitude of separation required for LC–MS quantitative bioanalysis of drugs and metabolites (Tan & Fanaras, 2018). Currently, the most extensively utilized LC–MS interfaces are based on atmospheric pressure ionization strategies, such as electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo‐ionization (APPI) (Sabourian et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…quantitative bioanalysis of drugs and metabolites (Tan & Fanaras, 2018). Currently, the most extensively utilized LC-MS interfaces are based on atmospheric pressure ionization strategies, such as electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo-ionization (APPI) (Sabourian et al, 2020).…”

mentioning

confidence: 99%

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, chiral, isotope-labeling, hydrophobicity-tailored and post-column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

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How much separation for LC–MS/MS quantitative bioanalysis of drugs and metabolites?

Cited by 7 publications

References 43 publications

Cladodes: Chemical and structural properties, biological activity, and polyphenols profile

Cladodes: Chemical and structural properties, biological activity, and polyphenols profile

Use of high‐pH (basic/alkaline) mobile phases for LC–MS or LC–MS/MS bioanalysis

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

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