How much of enzyme can be saved in the process with the optimal temperature control?

Grubecki, I.; Wójcik, Marek J.

doi:10.1016/j.jfoodeng.2012.12.019

Cited by 7 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Application of optimal temperature control in a batch bioreactor enables significant reduction of the reaction time [ 8 ], or the amount of the spent enzyme [ 9 ], when compared to an isothermal process. The advantages resulting from implementation of the optimal temperature control are the greater the higher is the value of the quotient of activation energy for enzyme deactivation process and that of reaction activation.…”

Section: Introductionmentioning

confidence: 99%

A new method to determine optimum temperature and activation energies for enzymatic reactions

Wójcik

Miłek

2016

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

A new method for determination of the optimum temperature and activation energies based on an idea of the average rate of enzymatic reaction has been developed. A mathematical model describing the effect of temperature on a dimensionless activity for enzyme deactivation following the first-order kinetics has been derived. The necessary condition for existence of the function extreme of the optimal temperature has been applied in the model. The developed method has been verified using the experimental data for inulinase from Kluyveromyces marxianus.

show abstract

Section: Introductionmentioning

confidence: 99%

A new method to determine optimum temperature and activation energies for enzymatic reactions

Wójcik

Miłek

2016

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…Temperature, besides feed flow rate, is the most important decisive variable in flow (bio)reactors [15,[50][51][52][53][54]. It has a crucial impact on enzyme activity, its stability, and in consequence on bioreactor performance [6].…”

Section: Optimization Problem Formulationmentioning

confidence: 99%

Analytical Determination of the Optimal Feed Temperature for Hydrogen Peroxide Decomposition Process Occurring in Bioreactor with a Fixed-Bed of Commercial Catalase

Grubecki

2020

Catalysts

Self Cite

View full text Add to dashboard Cite

The hydrogen peroxide-immobilized commercial catalase system was chosen to estimate the optimal feed temperature (OFT) for fixed-bed reactor (FXBR). This feed temperature was obtained based on analytical solution by maximizing the time-averaged substrate conversion under a constant feed flow rate and temperature constraints. In calculations a set of partial differential equations describing the conservation equation for fixed-bed reactor, assuming plug flow and kinetic equation for the rate of enzyme parallel deactivation was taken into account. The model is based on kinetic, and mass-transfer parameters estimated previously in a real decomposition process of hydrogen peroxide (HP). The simulation showed that the OFT is strongly dependent on hydrogen peroxide feed concentration, feed flow rate and diffusional resistances expressed by biocatalyst global effectiveness factor. It has been shown that the more significant diffusional resistances and the higher HP conversions are, the higher the optimal feed temperature is. The calculated values of the OFT were verified with the experimental results obtained in the model reactor at selected values of the feed flow rate. Presented analysis poses a significant simplification in a numerical computational procedure and can be very useful for engineers to select the temperature condition at which bioreactor productivity is expected to be maximal.

show abstract

“…Temperature is an important parameter, because each enzyme has its optimum reaction temperature [32], which can increase the productivity of the process. However, in the case of this work, an ambient temperature of 25 • C was considered.…”

Section: Parametermentioning

confidence: 99%

“…It shows the gradual loss of enzymatic activity, as it takes more time to reach 90% conversion. In the case of temperature [32], which can increase the productivity of the process. However, in the case of this work, an ambient temperature of 25 °C was considered.…”

Section: Parametermentioning

confidence: 99%

Simulation of the Reactivation of Partially Inactivated Biocatalysts in Sequential Batch Reactors

2020

View full text Add to dashboard Cite

The enzymatic reactivation process enables the recovery of catalytic activity for inactive biocatalysts. However, its effect on the specific productivity of the processes has not been studied. The main objective of this work was to evaluate the specific productivity of the processes with and without reactivation using the program Spyder Python (3.7). Using fixed values for all of the parameters, the global specific productivity was 8 mM/h·gbiocat for the process without reactivation, and 4 mM/h·gbiocat for the process with reactivation. Random numbers were generated to use as different values for parameters, and the results yielded a global specific productivity of 3.79 mM/h·gbiocat for the process with reactivation and 3.68 mM/h·gbiocat for the process without reactivation. ANOVA tests showed that there were significant differences between the specific global productivities of the two processes. Reactivation has great potential for use when the biocatalyst is of high cost.

show abstract

How much of enzyme can be saved in the process with the optimal temperature control?

Cited by 7 publications

References 24 publications

A new method to determine optimum temperature and activation energies for enzymatic reactions

A new method to determine optimum temperature and activation energies for enzymatic reactions

Analytical Determination of the Optimal Feed Temperature for Hydrogen Peroxide Decomposition Process Occurring in Bioreactor with a Fixed-Bed of Commercial Catalase

Simulation of the Reactivation of Partially Inactivated Biocatalysts in Sequential Batch Reactors

Contact Info

Product

Resources

About