How medium osmolarity influences dielectrophoretically assisted on-chip electrofusion

Hamdi, Feriel; Français, Olivier; Dufour-Gergam, Elisabeth; Pioufle, Bruno Le

doi:10.1016/j.bioelechem.2014.05.004

Cited by 12 publications

(10 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned in the literature , water defects are introduced within the membrane during the electropore formation, followed by a reorganization of the phospholipids head. Such water molecules introduction and bilayer reorganization lead to the increase of the membrane conductivity (σ mem ) , and permittivity . The lipid bilayer conductivity is linked to the increasing of both number and size of electropores .…”

Section: Resultsmentioning

confidence: 99%

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

2015

Self Cite

View full text Add to dashboard Cite

The electric field is commonly used in microdevices to handle, treat, or monitor living cells for various biological or biomedical applications (cells electrofusion, gene electrotransfer, drugs injection, cell sorting, …). Dielectrophoresis (DEP) forces, using stationary waves (conventional DEP) or traveling waves, are widely used for the cell handling or sorting. Electrorotation, which is induced by a rotating electrical field, is used for the determination of cell dielectric parameters. The application of pulsed electric field (PEF) results in the cell membrane permeabilization that might allow the transfer of various molecules in the cytoplasm. In this paper, we propose a method to monitor in situ the level of electropermeabilization induced by PEF application on a single cell, by combining the dielectrophoresis force and the electrorotation torque within a microfluidic device. The method was experimented on two different cell lines (human leukemic T-cell lymphoblast and murine melanoma cell): a single cell is captured by dielectrophoresis while its dielectric properties (both permittivity and conductivity of cytoplasm and membrane) are estimated thanks to a rotating electric field, which is applied simultaneously. The permeabilization effect of PEF, applied to the single cell trapped in such conditions in the biodevice, could be monitored by the estimation of its dielectric properties before and after pulse application.

show abstract

Section: Resultsmentioning

confidence: 99%

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Pucihar et al has reported that cell survival in experiments involving electropermeabilization can be improved by adjusting the medium conductivity [ 36 ]. On the other hand, studies suggest that isotonic medium is beneficial for cells viability when hypotonic buffer significantly improves electrofusion efficiency [ 37 , 38 ]. In this study, iso-osmotic medium with low conductivity (0.012S/m) was chosen to maximize cells viability.…”

Section: Discussionmentioning

confidence: 99%

A Cell Electrofusion Chip for Somatic Cells Reprogramming

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

Cell fusion is a potent approach to explore the mechanisms of somatic cells reprogramming. However, previous fusion methods, such as polyethylene glycol (PEG) mediated cell fusion, are often limited by poor fusion yields. In this study, we developed a simplified cell electrofusion chip, which was based on a micro-cavity/ discrete microelectrode structure to improve the fusion efficiency and to reduce multi-cell electrofusion. Using this chip, we could efficiently fuse NIH3T3 cells and mouse embryonic stem cells (mESCs) to induce somatic cells reprogramming. We also found that fused cells demethylated gradually and 5-hydroxymethylcytosine (5hmC) was involved in the demethylation during the reprogramming. Thus, the cell electrofusion chip would facilitate reprogramming mechanisms research by improving efficiency of cell fusion and reducing workloads.

show abstract

“…Cells shrivel in hypertonic solutions, resulting in biomolecular changes, (Alshareef et al 2013) which can affect the cell-COF (Hamdi et al 2014). To examine the effect of the solution tonicity on the cell-DEP, cells were exposed to a tonicity gradient inside the SNDA-DEP device.…”

Section: Tonicity Gradientmentioning

confidence: 99%

Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients

Ben-Arye

Park

Shemesh

et al. 2015

Biomed Microdevices

View full text Add to dashboard Cite

A novel design of reusable microfluidic platform that generates a stationary nanoliter droplet array (SNDA) for cell incubation and analysis, equipped with a complementary array of individually addressable electrodes for each microwell is studied. Various solute concentration gradients were generated between the wells where dielectrophoresis (DEP) was used to characterize the effect of the gradients on the cell's response. The feasibility of generating concentration gradients and observation of DEP responses was demonstrated using a gradient of salts in combination with microparticles and viable cells. L1210 Lymphoma cells were used as the model cells in these experiments. Lymphoma cells' cross-over frequency (COF) decreased with increasing stress conditions. Specifically, a linear decrease in the cell COF was measured as a function of solution tonicity and blebbistatin dose. Lymphoma cells were incubated under a gradient of the chemotherapeutic agent doxorubicin (DOX), which led to saturation in the cell-COF response at 30 nM DOX, demonstrating the potential of the platform in screening of label-free drugs.

show abstract

How medium osmolarity influences dielectrophoretically assisted on-chip electrofusion

Cited by 12 publications

References 55 publications

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

A Cell Electrofusion Chip for Somatic Cells Reprogramming

Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients

Contact Info

Product

Resources

About