How maternal age and environmental cues influence embryonic developmental pathways and diapause dynamics in a North American annual killifish

Abstract

Background Temporary pools are variable environments with seasonal drought/flood phases. Annual killifish have adapted to life in temporary pools by producing embryos that undergo diapause to traverse the dry phase. To fill existing knowledge gaps about embryo diapause regulation and evolution in annual killifishes, we test the effect of maternal age, incubation temperature, and incubation medium on diapause induction and length in Millerichthys robustus, the only North American fish species that has evolved an annual life history. Results All embryos at extreme temperatures follow a defined developmental pathway: skipping diapause at 30°C, and entering diapause at 18°C, both regardless of maternal age, and incubation medium. However, maternal age, and incubation medium influenced whether diapause is entered, and time arrested in diapause for embryos incubated at 25°C. At 25°C, five‐week‐old, and 52‐week‐old females produced more embryos that entered diapause than 26‐week‐old females. Also, embryos incubated in aqueous medium skipped diapause more frequently at this intermediate temperature. Conclusions Millerichthys developmental dynamics associated with maternal age under intermediate range of temperatures are likely adapted to the particular patterns of flood/drought in North American temporary pools. Millerichthys also exhibits developmental patterns largely comparable with other annual fishes, probably due to common seasonal patterns in temporary pools.

Effects of temperature and heat waves on embryonic development of annual fishes from Neotropical wetlands: Implications for climate change scenarios

“…However, the magnitude and direction of the effects of temperature changes on the development of annual fishes are not easy to predict. While low temperatures induced entry into diapause II, high temperatures induced escape from it in annual fish embryos (Domínguez‐Castanedo et al, 2022; Furness et al, 2015; Podrabsky et al, 2010). A previous study showed that the embryonic development of subtropical annual fish species was faster under higher temperatures (Arenzon et al, 2002).…”