How many species are there in the subgenus Bursaphis (Hemiptera: Sternorrhyncha: Aphididae)? CO-I evidence

Rakauskas, Rimantas; Turčinavičienė, Jurga; Bašilova, Jekaterina

doi:10.14411/eje.2011.060

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…In our case, this ratio ranged from 2.00 to 3.86 in the apple aphid, and for the spirea aphid it was 0.67-2.77 and 1.74-2.95 for samples from Europe and China, respectively (Table 5). dIScuSSIon Molecular markers are widely used to reveal cryptic insect species, including aphids (Rakauskas et al, 2011). Partial COI sequences used for DNA barcoding were analyzed for A. pomi (n = 76) and A. spiraecola (n = 56) by Foottit et al (2009).…”

Section: Dna Sequence Data Analysismentioning

confidence: 99%

Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities

Rakauskas¹,

Bašilova²,

Bernotienė³

2015

Eur. J. Entomol.

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Abstract. Aphid species Aphis pomi (de Geer, 1773) is oligophagous on pomoideous host plants, whilst Aphis spiraecola Patch, 1914 is a polyphagous species alternating between Spiraea spp., its primary host, and a wide variety of secondary hosts, also including pomoideous species. Despite the biological distinction, these species are difficult to separate using their morphological characters. Partial sequences of mitochondrial COI and nuclear EF-1α genes were analyzed for samples from Central and Eastern Europe, Germany, Bulgaria, Italy, Turkey, China together with available data from GenBank. Interspecific pairwise sample divergences of the COI fragment ranged from 3.1 to 4.3%. One COI haplotype of A. pomi was predominant (n = 24), with a pan European distribution. The most abundant COI haplotype of A. spiraecola (n = 16) occurred in Lithuania, Latvia, Poland, Italy, Turkey and China. Interspecific pairwise sample divergences of the EF-1α fragment ranged from 0.6 to 1.2%. Analyzed partial sequences of EF-1α were identical in A. pomi. The most abundant EF-1α haplotype of A. spiraecola (n = 14) occurred in Lithuania, Poland, Italy, Turkey and China. The length of ultimate rostral segment appeared to be the most reliable morphological character for discrimination between apple and spirea aphid species. It allowed a 100% correct identification of A. pomi (n = 143) and 91.5% of A. spiraecola (n = 94) specimens in the European samples used for the molecular analysis. The existence of A. spiraecola in the Eastern Baltic region of Europe is documented for the first time.

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Section: Dna Sequence Data Analysismentioning

confidence: 99%

Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities

Rakauskas¹,

Bašilova²,

Bernotienė³

2015

Eur. J. Entomol.

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“…The problems of morphological taxonomy of the gall-forming species identity, is confusing since they very closely related species, which are usually morphologically very similar and thus, making it difficult for identifying these species. (25,27,30). So the present study was done to avoid these problems.…”

Section: Resultsmentioning

confidence: 99%

Molecular Phylogeny of Gall-Forming Aphids Tribe Fordini (Homoptera, Aphididae, Eriosomatinae) on Pistacia Trees From Kurdistan Region North of Iraq

Ali¹

2020

Iraqi J. Agric. Sci.

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Four aphid species belonging to the Tribe Fordini (Homoptera, Aphididae, Eriosomatinae) induce galls on Pistacia trees in North Iraq (Kurdistan) were recorded in this study. Molecular phylogeny of Iraq Fordini species was constructed for These species (Forda riccobonii, Paracletus cimiciformis, Baizongia pistaciae and Forda marginata), based on sequences of mitochondrial Cytochrome Oxidase I (COI) gene of these aphid species to detect the relationship and taxonomy of Iraqi species for tribe Fordini. Our results confirmed that each of Forda and Paracletus are a sister genus, or monophyletic group by designing in same clade, while B. pistaciae is separate from other clades, supporting the taxonomic division of the tribe Fordini into two subtribes (Fordina and Baizongina).

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“…Molecular and genetic identification tools have been developed for H. vitripennis , which overcome the morphology limitation, targeting protein and genetic markers (de León et al , 2006; Fournier et al , 2006), including a polymerase chain reaction (PCR) assay that targets the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. The COI gene is a commonly used gene in molecular entomology as it can possess sufficient genetic resolution to distinguish between species and subspecies of organisms, as well as explore population genetics (Smith, 2005; Boykin et al , 2007; Malausa et al , 2011; Rakauskas et al , 2011). The COI gene has previously been used to evaluate the phylogeny of several Hemiptera suborders (Park et al , 2011; Li et al ., 2012; Foottit et al , 2014), and the common occurrence of this gene in publically accessible data repositories makes it an excellent starting point for assay design.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a real-time PCR assay for the glassy-winged sharpshooter Homalodisca vitripennis (Hemiptera: Cicadellidae)

Waite

Dsouza

et al. 2016

Bull. Entomol. Res.

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The glassy-winged sharpshooter (Homalodisca vitripennis) is an invasive pest organism, which is found throughout Central America and has recently invaded a few countries in the Pacific Islands. As a carrier of the highly virulent plant pathogenic bacterium Xylella fastidiosa, it is of great economic significance to horticulture and is estimated to cost Californian vineyards over US$100 million per year in control and losses. New Zealand is currently free from this pest, but its recent spread through the Pacific has raised concerns of it establishing in New Zealand, potentially as a result of introduction through human travel. We report here a real-time polymerase chain reaction assay for the rapid identification of H. vitripennis. The assay was extensively validated in silico then optimized and tested against a range of Cicadellidae species, both internationally collected and local to New Zealand. This assay was able to correctly identify H. vitripennis samples, and distinguish between H. vitripennis and close relatives, such as the smoke-tree sharpshooter (Homalodisca liturata) and will be of great benefit to New Zealand biosecurity.

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How many species are there in the subgenus Bursaphis (Hemiptera: Sternorrhyncha: Aphididae)? CO-I evidence

Cited by 12 publications

References 33 publications

Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities

Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities

Molecular Phylogeny of Gall-Forming Aphids Tribe Fordini (Homoptera, Aphididae, Eriosomatinae) on Pistacia Trees From Kurdistan Region North of Iraq

Development and validation of a real-time PCR assay for the glassy-winged sharpshooter Homalodisca vitripennis (Hemiptera: Cicadellidae)

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