How many podocyte autophagosomes are there in immunoglobulin A nephropathy and idiopathic membranous nephropathy?

Liang, Shikai; Jin, Juan; Gong, Jianguang; Lin, Bo; Li, Yiwen; He, Qiang

doi:10.1007/s11255-016-1398-5

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…We found that MPO is one of the top tightly interacting proteins confirmed by the protein-protein interaction network and a significantly upregulated protein (IgA/NC ratio: 5.675); furthermore, MPO is associated with the phagosome pathway [38], which is one of the three processes enriched in the IgAN patients and found that it was lower in the nephropathy group compared to the control group [41], which is the opposite the results identified in PBMCs. This finding has obscured whether there is a connection or contrast between podocytes and PBMCs.…”

Section: Discussioncontrasting

confidence: 57%

Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy

Huang

Zheng

Hua

et al. 2020

Preprint

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Protein posttranslational modification (PTM) is an indispensable regulatory element that can fine-tune protein functions and regulate diverse cellular processes. Lysine 2-hydroxyisobutyrylation (Khib) is a PTM that was recently identified and is thought to play a role in a wide variety of active cellular functions. In this report, for the first time, we comparatively studied the 2-hydroxyisobutyrylation proteome in peripheral blood mononuclear cells from a new-onset biopsy-proven immunoglobulin A nephropathy (IgAN) group and a normal control group based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). Altogether, 7405 proteins were identified and added to a Khib library. Of these proteins, we identified 111 with upregulated expression and 83 with downregulated expression. Furthermore, we identified 428 Khib modification sites on 290 Khib-modified proteins, including 171 sites with increased modification on 122 Khib-modified proteins and 257 specific sites with reduced modification on 168 Khib-modified proteins. Importantly, the abundance of lipocalin 2 was increased in the differentially expressed proteins, and a KEGG-based functional enrichment analysis showed that Khib proteins clustered in the IL-17 signaling pathway and phagosome category, which may have important associations with IgAN. Our data enlighten our understanding of Khib in IgAN and indicate that Khib may have important regulatory roles in IgAN.

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Section: Discussioncontrasting

confidence: 57%

Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy

Huang

Zheng

Hua

et al. 2020

Preprint

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“…Therefore, it was concluded that type I autophagy was correlated with histopathologically more progressive disease, possibly reflecting a tendency towards poorer prognosis. We have previously reported (25) that podocytes from the renal biopsies of IgAN patients possessed more autophagosomes than healthy subjects did. In this study, we found that autophagy was reduced in podocytes exposed to IgAN-derived aIgA1-conditioned medium compared to the CON group.…”

Section: Discussionmentioning

confidence: 88%

Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy

et al. 2018

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Triptolide is often used to treat patients with immunoglobulin A nephropathy (IgAN), especially in Asia. However, its detailed mechanism remains unclear. In vitro experiments were conducted with podocytes exposed to aggregated IgA (aIgA)-MSC1097-conditioned media. A total of four groups were compared in this study: A control group (CON); a healthy supernatant group (HEAs); an IgAN supernatant group (IgANs); and a triptolide group (TRI). First, aggregated IgA1 (aIgA1) was generated by heating monomeric IgA1 (mIgA1) from IgAN patients or healthy subjects. Next, the conditioned supernatant of MSC-1097 cells cultured with aIgA1 (100 mg/l) from IgAN patients (IgANs) or healthy subjects (HEAs) or without aIgA1 (CON) were harvested and used to incubate MPC5 cells. MPC5 cells in the TRI group were cultured with triptolide (10 ng/ml) and conditioned media from MSC-1097 cells cultured with aIgA1 from IgAN patients. After 24 h of treatment, MPC5 cells were collected to measure autophagy-related protein levels, including microtubule-associated protein light chain 3 (LC3), p62, cluster of differentiation (CD)63, phosphorylated-protein kinase B (Akt), Akt, p-mammalian target of rapamycin (mTOR), and mTOR, via western blotting, immunofluorescence or both, and to determine apoptosis by flow cytometry. All the results showed no difference between the CON and the HEAs. Compared to the CON and the HEAs, MPC5 cells in the IgANs group showed reduced autophagy, which was presented as decreased levels of LC3-II and CD63, as well as accumulation of p62, and an increased podocyte apoptosis rate. This was partly rescued by the addition of triptolide. Moreover, the p-mTOR/mTOR ratio increased in the IgANs group and decreased in the TRI group. Therefore, these results suggest that triptolide protects podocyte autophagy in IgAN patients.

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“…In the physiological state, a basal level of autophagy is required for the removal of unfolded or misfolded proteins and for protein degradation for amino acid production, among others, so as to maintain the stability of the intracellular environment [5]. Although autophagic cell death plays an important role in disease occurrence and development [5], a moderate amount of autophagy is required for kidney development and podocyte differentiation, and an imbalance of autophagy is closely related to the occurrence of glomerular lesions, IgA nephropathy, idiopathic membranous nephropathy, and other diseases [6,7].…”

Section: Introductionmentioning

confidence: 99%

Shensu IV prevents glomerular podocyte injury in nephrotic rats via promoting lncRNA H19/DIRAS3-mediated autophagy

Huang

Zhou

et al. 2021

Bioscience Reports

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Shensu IV is a Chinese prescription well-known for its function in treating chronic kidney diseases. However, the potential mechanisms underlying how Shensu IV exerts its effects remain unclear. In the present study, we investigated the effects of Shensu IV on glomerular podocyte injury in nephrotic rats and puromycin-induced injury in cultured podocytes, and assessed the associated molecular mechanisms. Liquid chromatography–mass spectrometry (LC–MS) results showed that the main components of Shensu IV were l-Carnitine, P-lysoPC (LPC) 16:0, Coumaroyl tyramine, Tetramethylpyrazine, LPC 18:1, Choline, (S,S)-Butane-2,3-diol, and Scopoletin. We further found that nephrotic rats displayed pathological alterations in kidney tissues and ultrastructural changes in glomerular podocytes; however, these effects were reversed with Shensu IV treatment. Compared with the control, the numbers of autophagosomes were markedly reduced in the model group, but not in the Shensu IV treatment group. Furthermore, the expression of p62 was significantly higher in the model group than in the controls, whereas the LC3-II/I ratio was significantly lower; however, these changes were not observed when Shensu IV was administered. The protective effects of Shensu IV were further confirmed in podocytes displaying puromycin-induced injury. Compared with control group, the expression of long non-coding RNA (lncRNA) H19, mTOR, p-mTOR, and p62 was significantly increased in the puromycin group, whereas that of distinct subgroup of the RAS family member 3 (DIRAS3) was significantly decreased, as was the LC3-II/I ratio. The opposite results were obtained for both shH19- and Shensu IV-treated cells. Collectively, our data demonstrated that Shensu IV can prevent glomerular podocyte injury in nephrotic rats and puromycin-treated podocytes, likely via promoting lncRNA H19/DIRAS3-regulated autophagy.

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How many podocyte autophagosomes are there in immunoglobulin A nephropathy and idiopathic membranous nephropathy?

Abstract: Therefore, the results of the present study indicate that autophagy participates in podocyte injury and the progression of IgAN and IMN.

Cited by 15 publications

References 23 publications

Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy

Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy

Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy

Shensu IV prevents glomerular podocyte injury in nephrotic rats via promoting lncRNA H19/DIRAS3-mediated autophagy

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