2017
DOI: 10.1016/j.bbamem.2017.08.016
|View full text |Cite
|
Sign up to set email alerts
|

How kanamycin A interacts with bacterial and mammalian mimetic membranes

Abstract: Biological membranes are natural barriers to the transport of molecules and drugs within human bodies. Many antibacterial agents need to cross these membranes to reach their target and elicit specific effects. Kanamycin A belongs to the family of aminoglycoside antibiotics that target cellular RNA to inhibit bacterial and viral replication. Previous studies have shown that aminoglycosides bind to mammalian but disrupt bacterial membranes. In this study, molecular dynamics (MD) simulations and infrared (IR) spe… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
33
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
7
2

Relationship

2
7

Authors

Journals

citations
Cited by 38 publications
(36 citation statements)
references
References 83 publications
0
33
0
Order By: Relevance
“…The cell viability started to decrease after around 144 h ( Figure 3A , closed green stars) and reached zero after 312 h. However, considering the progress of the VCC, it was most likely that the viability reached zero already after about 264 h. In this experiment, kanamycin was supplemented to the medium (see Supplementary Table S1 ). Kanamycin disrupts bacterial membranes and binds to the cellular membrane of mammalian cells ( John et al, 2017 ). As shown, the viability was above 97% for the first 115 h of the cultivation, indicating that the addition of kanamycin did not affect the viability measurement by trypan blue exclusion.…”
Section: Resultsmentioning
confidence: 99%
“…The cell viability started to decrease after around 144 h ( Figure 3A , closed green stars) and reached zero after 312 h. However, considering the progress of the VCC, it was most likely that the viability reached zero already after about 264 h. In this experiment, kanamycin was supplemented to the medium (see Supplementary Table S1 ). Kanamycin disrupts bacterial membranes and binds to the cellular membrane of mammalian cells ( John et al, 2017 ). As shown, the viability was above 97% for the first 115 h of the cultivation, indicating that the addition of kanamycin did not affect the viability measurement by trypan blue exclusion.…”
Section: Resultsmentioning
confidence: 99%
“…This is less than the typical value for human erythrocyte membranes, and therefore is physiologically relevant. The protective effect of cholesterol is commonly attributed to an increased membrane cohesion and mechanical stiffness, but potentially also to the change in membrane surface charge …”
Section: Resultsmentioning
confidence: 99%
“…Moreover, the primary structural difference between geneticin and kanamycin is the presence of 2 additional methyl groups that confer increased hydrophobicity (Kaplan et al, 2016). Furthermore, recent work indicates that the ability of kanamycin to enter cells is different in bacteria vs. eukaryotic cells (John et al, 2017). We posit that the historically evidenced reduced efficacy of kanamycin in S. cerevisiae may be the result of inability of kanamycin to enter the cell, which in our hands, is significantly reduced when kanamycin is combined with IL.…”
Section: Discussionmentioning
confidence: 64%