How ionic liquids can help to stabilize native proteins

Weingärtner, Hermann; Cabrele, Chiara; Herrmann, Christian

doi:10.1039/c1cp21947b

Cited by 254 publications

(245 citation statements)

References 98 publications

(253 reference statements)

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“…The activity and stability of enzymes in ILs system can be significantly affected by the hydrophobicity of ILs [48]. Researchers observed that the enzyme could achieve higher activity and stability in more hydrophobic ILs.…”

Section: Effect Of Hydrophobicity Of Ionic Liquidmentioning

confidence: 99%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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Abstract:Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P, functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation.

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Section: Effect Of Hydrophobicity Of Ionic Liquidmentioning

confidence: 99%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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“…Their application as media for maintaining the protein stability has been extensively studied, either employed as co-solvents with water, in biphasic systems, or as "neat" solvents [8][9][10][11]. Some reports also demonstrated that some ILs are able to maintain or even increase the proteins stability [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids

Taha

Quental

Correia

et al. 2015

Process Biochemistry

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Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures. In this work, the extraction ability for bovine serum albumin (BSA) of aqueous biphasic systems (ABS) formed by polypropylene glycol 400 (PPG 400) and several GB-ILs was evaluated. ABS formed by PPG 400 and cholinium chloride ([Ch]Cl), GBs, and sucrose were also investigated for comparison purposes. It is shown that BSA preferentially migrates for the GB-IL-rich phase, with extraction efficiencies of 100%, achieved in a single-step. Dynamic light scattering, and circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies were employed to evaluate the effect of the investigated cholinium-based GB-ILs on the BSA stability, and compared with results obtained for the respective GBs precursors, [Ch]Cl and sucrose, a well-known protein stabilizer. Molecular docking studies were also carried out to investigate on the binding sites of GB-IL ions to BSA. The experimental results confirm that BSA has a higher stability in GB-ILs than in any of the other compounds investigated.

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“…77 It is believed that enzymes can retain a residual hydration shell in non-polar solvents which stabilises the native fold. 79 Due to their similar physicochemical properties, it was thus evident to investigate deep eutectic solvents as reaction media for biotransformations. Gorke et al were the first to use enzymes in DESs and to assess the activity of different hydrolases in choline chloride-urea mixtures.…”

Section: Biocatalytic Reactionsmentioning

confidence: 99%