How hsa‐miR‐495 performed in the tumorigenesis of pancreatic adenocarcinoma by bioinformatics analysis

Abstract

Introduction Pancreatic adenocarcinoma (PAAD) is one of the most fatal cancers in the world for early metastasis, extensive invasion, and poor prognosis with a 5‐year survival rate less than 5%. However, the underlying mechanisms are poorly understood. Therefore, it is urgent to explore molecular markers for early diagnosis or therapy target to improve the outcome of PAAD. Methods We retrieved transcriptome data as well as clinical information from patients with PAAD in The Cancer Genome Altas (TCGA) database. Survival time associated microRNAs (miRNAs) and messenger RNAs (mRNAs) were initially identified, followed by enrichment analysis (Gene Ontology [GO] and pathway). The relationship between survival time associated miRNAs‐mRNAs was also investigated to discover putative transcriptional control mechanisms of PAAD. Finally, by consulting the literature and retrieving the database, we found that hsa‐miR‐495 might have played an important role in PAAD. Results In total, 146 miRNAs from 378 miRNAs and 580 mRNA from 17 100 mRNA, including 328 risk mRNA and 252 protective mRNA, were found to be associated with the survival time of PAAD. Eight hundred eighty‐eight mRNA‐miRNA pairs were related to the survival time of PAAD, involving in 755 mRNAs and 35 miRNAs. We chose 13 miRNAs predicted by target gene in the miRanda database for further research. Among these 13 miRNAs, hsa‐miR‐495 was identified as a good biomarker. Through GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, the significantly enriched pathways involved in focal adhesion, Staphylococcus aureus infection, and Intestinal immune network for immunoglobulin A production. And four target genes and 87 pathways of the hsa‐miR‐495 were enriched in PAAD. Interestingly, we found hsa‐miR‐495 with a low expression having a poor overall survival and significantly different recurrence rate within 5 years. Conclusion Hsa‐miR‐495 and its target genes may serve as a prognostic and predictive marker in PAAD. Further research on the function of the hsa‐miR‐495 and its target genes in the KEGG pathway may provide references for treatment of PAAD.

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

“…MicroRNA-543 was found to be low expressed in 160 PC patients using TCGA database [20]. And by the starBase database, we also nd that microRNA-543 was downregulated in PC tissues and with poor survival (Fig.…”

“…MicroRNA-543 was found abnormal transcription and associated with poor prognosis of PC by the TCGA database with 160 PC patients [20], and the satrBase 3.0 (http://starbase.sysu.edu.cn) database and Kaplan Meier-plotter (http://kmplot.com/analysis/index.php?p=service) also showed the same results.…”

“…Zhang et al reported that microRNA-543 inhibited cervical cancer growth and metastasis by targeting TRPM7 [7]. Other research have also found that microRNA-543 can control the disease development of cancer, lung cancer, cervical cancer, and colorectal cancer [5,7,8,25] [20]. But they didn't do a thorough study, and we did a deep study of the mechanism of this result.…”

MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis

“…MicroRNA-543 was found to have abnormal transcription and was associated with poor prognosis in 160 PC patients from the The Cancer Genome Atlas (TCGA) database ( 21 ). The StarBase 3.0 ( http://starbase.sysu.edu.cn ) database and Kaplan-Meier plotter ( http://kmplot.com/analysis/index.php?p=service ) also showed the same results.…”

Metastatic pancreatic adenocarcinomas could be classified into M1a and M1b category by the number of metastatic organs

“…Pancreatic cancer (PC), the fourth leading cause of cancerrelated deaths in the United States, is a highly lethal and aggressive malignancy with a 5-year survival rate of less than 5% [1,2]. Approximately half of PC patients have developed distant metastasis at the time of first diagnosis [3].…”