How Hormones and MADS-Box Transcription Factors Are Involved in Controlling Fruit Set and Parthenocarpy in Tomato

Molesini, Barbara; Dusi, Valentina; Pennisi, Federica; Pandolfini, Tiziana

doi:10.3390/genes11121441

Cited by 30 publications

(29 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, GH3.1 may be an important part in balancing the auxin synthesis and metabolism to ensure a fruit set in "R35-P" under any favorable or unprofitable conditions. Overall, these studies revealed that parthenocarpy can be achieved by manipulating auxin action at different levels, by acting on its biosynthesis, signaling cascade, and transport, corroborating the crucial role played by this hormone in the control of a fruit set (Molesini et al, 2020). Taken together, these results indicated that auxins played a pivotal role in controlling the fruit set of "R35-P."…”

Section: Discussionsupporting

confidence: 57%

“…LAX2 related to IAA transport with a possible role in the regulation of auxin influx ( Pattison and Catalá, 2012 ). As IAA-amido synthetase, GH3 can promote an IAA conjugation to amino acids to maintain auxin homeostasis ( Molesini et al, 2020 ). In this study, GH3.1 may be an important part in balancing the auxin synthesis and metabolism to ensure a fruit set in “R35-P” under any favorable or unprofitable conditions.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Auxin Metabolism Is Involved in Fruit Set and Early Fruit Development in the Parthenocarpic Tomato “R35-P”

Zhang

Shao

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Parthenocarpic tomato can set fruit and develop without pollination and exogenous hormone treatments under unfavorable environmental conditions, which is beneficial to tomato production from late fall to early spring in greenhouses. In this study, the endogenous hormones in the ovaries of the parthenocarpic tomato line “R35-P” (stigma removed or self-pollination) and the non-parthenocarpic tomato line “R35-N” (self-pollination) at four stages between preanthesis and postanthesis investigated, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A nearly twofold IAA (indoleacetic acid) content was found in “R35-P” rather than in “R35-N” at −2 and 0 days after anthesis (DAA). Except at −2 DAA, a lower ABA (abscisic acid) content was observed in Pe (stigma removed in “R35-P”) compared to that in Ps (self-pollination in “R35-P”) or CK (self-pollination in “R35-N”). After pollination, although the content of GA1 (gibberellins acid 1) in CK increased, the levels of GAs (gibberellins acids) were notably low. At all four stages, a lower SA (salicylic acid) content was found in Ps and CK than in Pe, while the content and the change trend were similar in Ps and CK. The variation tendencies of JA (jasmonic acid) varied among Pe, Ps, and CK at the studied periods. Furthermore, KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses of transcriptomic data identified 175 differentially expressed genes (DEGs) related to plant hormone signal transduction, including 63 auxin-related genes, 27 abscisic acid-related genes, 22 ethylene-related genes, 16 cytokinin-related genes, 16 salicylic acid-related genes, 14 brassinosteroid-related genes, 13 jasmonic acid-related genes, and 4 gibberellin-related genes at −2 DAA and 0 DAA. Our results suggest that the fate of a fruit set or degeneration occurred before anthesis in tomato. Auxins, whose levels were independent of pollination and fertilization, play prominent roles in controlling a fruit set in “R35-P,” and other hormones are integrated in a synergistic or antagonistic way.

show abstract

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Auxin Metabolism Is Involved in Fruit Set and Early Fruit Development in the Parthenocarpic Tomato “R35-P”

Zhang

Shao

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Besides, boron deficiency can affect pollen germination, pollen tube growth, fruit set, and seed formation, thereby inhibiting reproductive growth ( Alva et al, 2015 ). Previous studies on seedless phenotype were principally focused on other species, such as Arabidopsis ( Goetz et al, 2007 ), tomato ( Klap et al, 2017 ; Takisawa et al, 2018 ; Molesini et al, 2020 ), grapevine ( Malabarba et al, 2017 ; di Rienzo et al, 2021 ; Li et al, 2021 ), citrus ( Zhang et al, 2018 ; Liang et al, 2020 ), eggplant ( Chen et al, 2017 ), sugar apple ( Lora et al, 2011 ), and pear ( Wang et al, 2021a , b ).…”

Section: Introductionmentioning

confidence: 99%

“…The authors found two independent mutations in MADS-box transcription factor SHELL , a homolog of the MADS-box gene SEEDSTICK (also known as AGAMOUS-Like 11, AGL11 ), which acts on the ovule identity and seed development in Arabidopsis ( Singh et al, 2013 , 2020 ). In addition, MADS-box genes have also been reported to be implicated in pollen fertilization and the development of seedless fruits ( Klap et al, 2017 ; Takisawa et al, 2018 ; Tang et al, 2019 ; Wei et al, 2019 ; Molesini et al, 2020 ). MADS-box gene AGL11 has been proposed as the key gene responsible for triggering the seedless phenotype ( Ocarez and Mejía, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Programmed Cell Death May Be Involved in the Seedless Phenotype Formation of Oil Palm

et al. 2022

View full text Add to dashboard Cite

Oil palm (Elaeis guineensis Jacq.) is a well-known vegetable oil-yielding crop. Seedlessness is one of the most prominent traits in oil palm due to its low processing costs and high oil content. Nevertheless, an extensive study on molecular mechanisms regulating seedless phenotype formation in oil palm is very limited so far. In this study, stigma, style, and ovary from seedless and seeded (Tenera and Pisifera) oil palm trees were used to investigate the possible mechanism. Results showed that non-pollination resulted in no fruits, and self- and cross-pollinations resulted in seedless fruits, while boron treatment had no effect on seedless phenotype formation, implying that seedless trees have incomplete self and outcrossing incompatibility. Furthermore, the transcriptome data analysis highlighted eight programmed cell death (PCD) genes and three groups of PCD-related genes: 4-coumarate-CoA ligase (4CL), S-RNase, and MADS-box. The majority of these genes were significantly up-regulated in the stigma and style of Seedless palm trees compared to Tenera and Pisifera. In addition, the co-expression network analysis confirmed the significant correlation among these genes. Moreover, two simple sequence repeats (SSR) markers (S41 and S44) were developed to identify the seedless phenotype. The up-regulation of 4CL and MADS-box TFs activated the expression of PCD genes; on the other hand, S-RNase resulted in pollen tube RNA degradation and triggered PCD. While the link between PCD and seedless phenotype formation in oil palm has not been extensively studied to date, these findings suggest a role of PCD in pollen tube lethality, leading to double fertilization failure and the seedless phenotype.

show abstract

“…A peculiar and distinctive phenotype of the tomato Sltpd1 mutants is the formation of seedless fruits (parthenocarpic). Parthenocarpy, the formation of fruits in the absence of pollination and fertilization, is often the consequence of the precocious activation of molecular events normally triggered by these processes (Molesini et al, 2020). Also, it could be achieved by external applications of different hormones or growth regulators (Vivian-Smith and Koltunow, 1999).…”

Section: Discussionmentioning

confidence: 99%

SlTDP1 Is Required To Specify Tapetum Identity And For The Regulation Of Redox Homeostasis In Tomato Anthers

Salazar-Sarasua

López-Martín

Roque³

et al. 2021

Preprint

View full text Add to dashboard Cite

The tapetum is a specialized layer of cells within the anther adjacent to the sporogenic tissue. During its short life, it provides nutrients, molecules and materials to the pollen mother cells and microsporocytes being essential during callose degradation and pollen wall formation. However, the acquisition of tapetal cell identity in tomato plants is a process still poorly understood. We report here the identification and characterization of SlTPD1 (Solanum lycopersicum TPD1), a gene specifically required for pollen development in tomato plants. Gene editing was used to generate loss-of-function Sltpd1 mutants that showed absence of tapetal tissue. In these plants, sporogenous cells developed but failed to complete meiosis resulting in complete male sterility. Transcriptomic analysis conducted in wild-type and mutant anthers at an early stage revealed the down regulation of a set of genes related to redox homeostasis. Indeed, Sltpd1 anthers showed a reduction of reactive oxygen species (ROS) accumulation at early stages and altered activity of ROS scavenging enzymes. The obtained results highlight the importance of ROS homeostasis in the interaction between the tapetum and the sporogenous tissue in tomato plants.One sentence summaryThe small protein SlTPD1 is required for tapetum formation in tomato, highlighting the role of this tissue in the regulation of redox homeostasis during male gametogenesis.

show abstract

How Hormones and MADS-Box Transcription Factors Are Involved in Controlling Fruit Set and Parthenocarpy in Tomato

Cited by 30 publications

References 99 publications

Auxin Metabolism Is Involved in Fruit Set and Early Fruit Development in the Parthenocarpic Tomato “R35-P”

Auxin Metabolism Is Involved in Fruit Set and Early Fruit Development in the Parthenocarpic Tomato “R35-P”

Programmed Cell Death May Be Involved in the Seedless Phenotype Formation of Oil Palm

SlTDP1 Is Required To Specify Tapetum Identity And For The Regulation Of Redox Homeostasis In Tomato Anthers

Contact Info

Product

Resources

About