How does vitrification affect oocyte viability in oocyte donation cycles? A prospective study to compare outcomes achieved with fresh versus vitrified sibling oocytes

Solé, Miquel; Santaló, Josep; Boada, Montserrat; Clua, Elisabet; Rodríguez, Ignacio; Martínez, Francisca; Coroleu, Buenaventura; Barri, P.N.; Veiga, Anna

doi:10.1093/humrep/det242

Cited by 111 publications

(65 citation statements)

References 25 publications

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“…In this study, oocytes obtained from CD-1 mice (all of the same genetic background), could better explore the influence of vitrification, and no significant difference in the transcription of oocytes after vitrification was found. To some extent, these results are consistent with a previous report that vitrified oocytes from the same cohort of donor oocytes (oocytes from same donor(s)) and showed no statistically significant differences in fertilization, embryo quality or clinical results when compared with fresh oocytes [28][29][30] .…”

Section: Discussionsupporting

confidence: 91%

RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification

Gao

Jia

et al. 2018

Cryobiology

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Section: Discussionsupporting

confidence: 91%

RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification

Gao

Jia

et al. 2018

Cryobiology

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“…Oocyte survival using the closed Rapid-i tool was similar to reports for open systems using the same cryoprotectants [4,[29][30][31][32][33][34]. Limited previous experience with a variety of closed systems (not Rapid-i) has generated survival rates ranging from 60 to 90 % [18,24,35,36].…”

Section: Discussionsupporting

confidence: 58%

Closed vitrification of human oocytes and blastocysts: outcomes from a series of clinical cases

Gook

Choo

Bourne

et al. 2016

J Assist Reprod Genet

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Purpose High survival rates and clinical outcomes similar to those from fresh oocytes and blastocysts have been observed with open oocyte vitrification systems. It has been suggested that the extremely fast cooling rates that are only achieved with open systems are necessary for human oocyte and blastocyst vitrification. However, there is a potential risk of introducing contamination with open systems. The aim of this study was to assess whether similar survival and subsequent implantation rates could be achieved using a closed vitrification system for human oocytes and blastocysts. Methods Initially, donated immature oocytes that were matured in vitro were vitrified using the cryoprotectants ethylene glycol (EG) + dimethyl sulphoxide (DMSO) + sucrose and either a closed system (Rapid-i®) or an open system (Cryolock). The closed system was subsequently introduced clinically for mature oocyte cryopreservation cases and blastocyst vitrification. Results Using in vitro matured oocytes, a similar survival was achieved with the open system of 92.4 % (73/79) and with the closed system of 89.7 % (35/39). For clinical oocyte closed vitrification, high survival rate of 90.5 % (374/413) and an implantation rate of 32.7 % (18/55) from the transfer of day 2 embryos was achieved, which is similar to fresh day 2 embryo transfers. Blastocysts have also been successfully cryopreserved using the Rapid-i closed vitrification system with 94 % of blastocysts having an estimated ≥75 % of cells intact and a similar implantation rate (31.5 %) to fresh single blastocyst transfers. Conclusion Closed vitrification can achieve high survival and similar implantation rates to fresh for both oocytes and blastocysts.

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“…Currently it is estimated that around 20 MII oocytes are required per child [14][15][16], which means that only a minority of these patients may benefit from this option. Therefore, IVM matured oocytes in this way may serve as an add-on method to ovarian tissue freezing, especially in young patients and in those centers choosing to excise one whole ovary for fertility preservation, but IVM in this manner cannot be recommended as a single method of fertility preservation.…”

Section: Discussionmentioning

confidence: 99%

Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

Yin

Jiang

Kristensen

et al. 2016

J Assist Reprod Genet

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Purpose The aim of the study was to investigate the maturation rate of immature oocytes collected from ovarian medulla tissue normally discarded during preparation of ovarian cortical tissue for fertility preservation. Further we evaluated survival of derived MII oocytes following vitrification and warming. Methods 36 patients aged from 8 to 41 years who had one ovary excised for fertility preservation were included. Oocytes were collected from the medulla tissue and matured in vitro 44-48 h followed by vitrification. Number of oocytes collected, the rates of maturation and post-warming survival were assessed. Results On average, 11 immature oocytes were collected per patient. The overall maturation rate was 29 % irrespective of whether the ovary was transported 4-5 h on ice or obtained immediately after oophorectomy. The maturation rate in patients below 20 years of age (55 %) was significantly higher than that of patients aged 20-30 years (29 %) and above 30 years (26 %). The post-warming survival rate was 64 %. No significant relationship was observed between the number of collected oocytes and the age of patients.Conclusions Approximately three MII oocytes were obtained per patient following in vitro maturation (IVM) of immature oocytes collected from medulla tissue, of which two survived vitrification and warming. This approach represents an add-on method to potentially augment the fertility opportunity for cancer patients, especially in young women with cancer where transplantation of cortical tissue may pose a risk of relapse, but the IVM approach is currently too inefficient to be the only method used for fertility preservation.

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How does vitrification affect oocyte viability in oocyte donation cycles? A prospective study to compare outcomes achieved with fresh versus vitrified sibling oocytes

Cited by 111 publications

References 25 publications

RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification

RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification

Closed vitrification of human oocytes and blastocysts: outcomes from a series of clinical cases

Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

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